{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:19:06Z","timestamp":1760710746296,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1602102-1"],"award-info":[{"award-number":["2018YFC1602102-1"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key R&amp;D Program of Guangxi","award":["AB20999005"],"award-info":[{"award-number":["AB20999005"]}]},{"name":"the Fundamental Research Funds for the Academy of National Food and Strategic Re-serves Administration","award":["ZX1913"],"award-info":[{"award-number":["ZX1913"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803712, 31901806"],"award-info":[{"award-number":["81803712, 31901806"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A rapid and high-throughput fluorescence detection method for zearalenone (ZEN) based on a CuO nanoparticle (NP)-assisted signal amplification immunosensor was developed using an automated sample pretreatment and signal conversion system. CuO NPs with high stability and biocompatibility were used as carriers to immobilize anti-ZEN antibodies. The obtained CuO NP-anti-ZEN can maintain the ability to recognize target toxins and act as both a signal source and carrier to achieve signal conversion using automated equipment. In this process, target toxin detection is indirectly transformed to Cu2+ detection because of the large number of Cu2+ ions released from CuO NPs under acidic conditions. Finally, a simple and high-throughput fluorescence assay based on a fluorescent tripeptide molecule was employed to detect Cu2+, using a multifunctional microporous plate detector. A good linear relationship was observed between the fluorescence signal and the logarithm of ZEN concentration in the range of 16.0\u20131600.0 \u03bcg\/kg. Additionally, excellent accuracy with a high recovery yield of 99.2\u2013104.9% was obtained, which was concordant with the results obtained from LC-MS\/MS of naturally contaminated samples. The CuO NP-based assay is a powerful and efficient screening tool for ZEN detection and can easily be modified to detect other mycotoxins.<\/jats:p>","DOI":"10.3390\/s21196538","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T21:26:20Z","timestamp":1633728380000},"page":"6538","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection"],"prefix":"10.3390","volume":"21","author":[{"given":"Zhihong","family":"Xuan","sequence":"first","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]},{"given":"Yanxiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]},{"given":"Hongmei","family":"Liu","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9619-4943","authenticated-orcid":false,"given":"Jin","family":"Ye","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]},{"given":"Songxue","family":"Wang","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Habschied, K., Kanizai Saric, G., Krstanovic, V., and Mastanjevic, K. 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