{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:47:28Z","timestamp":1777729648924,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJCR14G2"],"award-info":[{"award-number":["JPMJCR14G2"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJOP1834"],"award-info":[{"award-number":["JPMJOP1834"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18H03778"],"award-info":[{"award-number":["JP18H03778"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Towards clarifying the spatio-temporal neurotransmitter distribution, potentiometric redox sensor arrays with 23.5-\u00b5m resolution were fabricated. The sensor array based on a charge-transfer-type potentiometric sensor comprises 128\u00d7128 pixels with gold electrodes deposited on the surface of pixels. The sensor output corresponding to the interfacial potential of the electrode changed logarithmically with the mixture ratio of K3Fe(CN)6 and K4Fe(CN)6, where the redox sensitivity reached 49.9 mV\/dec. By employing hydrogen peroxidase as an enzyme and ferrocene as an electron mediator, the sensing characteristics for hydrogen peroxide (H2O2) were investigated. The analyses of the sensing characteristics revealed that the sensitivity was about 44.7 mV\/dec., comparable to the redox sensitivity, while the limit of detection (LOD) was achieved to be 1 \u00b5M. Furthermore, the oxidation state of the electron mediator can be the key to further lowering the LOD. Then, by immobilizing oxidizing enzyme for H2O2 and glutamate oxidase, glutamate (Glu) measurements were conducted. As a result, similar sensitivity and LOD to those of H2O2 were obtained. Finally, the real-time distribution of 1 \u00b5M Glu was visualized, demonstrating the feasibility of our device as a high-resolution bioimaging technique.<\/jats:p>","DOI":"10.3390\/s21227682","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T02:43:31Z","timestamp":1637289811000},"page":"7682","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Redox Sensor Array with 23.5-\u03bcm Resolution for Real-Time Imaging of Hydrogen Peroxide and Glutamate Based on Charge-Transfer-Type Potentiometric Sensor"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9846-297X","authenticated-orcid":false,"given":"Tatsuya","family":"Iwata","sequence":"first","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"},{"name":"Department of Electrical and Electronic Engineering, Toyama Prefectural University, Imizu 9390398, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Okumura","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Okumura","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Horio","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Doi","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuaki","family":"Sawada","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 4418580, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.trac.2016.11.001","article-title":"Current advancement in electrochemical analysis of neurotransmitters in biological fluids","volume":"86","author":"Hasanzadeh","year":"2017","journal-title":"TrAC-Trends Anal. 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