{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:44:27Z","timestamp":1780580667353,"version":"3.54.1"},"reference-count":49,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Cadmium (Cd2+) is one of the most toxic heavy metals causing serious health problems; thus, designing accurate analytical methods for monitoring such pollutants is highly urgent. Herein, we report a label-free electrochemical aptasensor for cadmium detection in water. For this, a nanocomposite combining the advantages of gold nanoparticles (AuNPs), carbon nanotubes (CNTs) and chitosan (Cs) was constructed and used as immobilization support for the cadmium aptamer. First, the surface of a glassy carbon electrode (GCE) was modified with CNTs-CS. Then, AuNPs were deposited on CNTs-CS\/GCE using chrono-amperometry. Finally, the immobilization of the amino-modified Cd-aptamer was achieved via glutaraldehyde cross-linking. The different synthesis steps of the AuNPs\/CNTs\/CS nano assembly were characterized by cyclic voltammetry (CV). Electrochemical impedance spectroscopy (EIS) was employed for cadmium determination. The proposed biosensor exhibited excellent performances for cadmium detection at a low applied potential (\u22120.5 V) with a high sensitivity (1.2 K\u03a9\u00b7M\u22121), a detection limit of 0.02 pM and a wide linear range (10\u221213\u201310\u22124 M). Moreover, the aptasensor showed a good selectivity against the interfering ions: Pb2+; Hg2+ and Zn2+. Our electrochemical biosensor provides a simple and sensitive approach for Cd2+ detection in aqueous solutions, with promising applications in the monitoring of trace amounts of heavy metals in real samples.<\/jats:p>","DOI":"10.3390\/s22010105","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T01:06:54Z","timestamp":1640567214000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Fabrication of AuNPs\/MWCNTS\/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water"],"prefix":"10.3390","volume":"22","author":[{"given":"Selma","family":"Rabai","sequence":"first","affiliation":[{"name":"Laboratory of Sensors, Instrumentations and Process (LCIP), University of Khenchela, Khenchela 40000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1336-3057","authenticated-orcid":false,"given":"Ahlem","family":"Teniou","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7987-7945","authenticated-orcid":false,"given":"Ga\u00eblle","family":"Catanante","sequence":"additional","affiliation":[{"name":"Laboratoire Biocapteurs, Analyse et Environnement (BAE), Universit\u00e9 de Perpignan Via Domitia, 66860 Perpignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Messaoud","family":"Benounis","sequence":"additional","affiliation":[{"name":"Laboratory of Sensors, Instrumentations and Process (LCIP), University of Khenchela, Khenchela 40000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Marty","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Rhouati","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1039\/C3AN01840G","article-title":"Aptasensor based on the synergistic contributions of chitosan\u2013gold nanoparticles, graphene\u2013gold nanoparticles and multi-walled carbon nanotubes-cobalt phthalocyanine nanocomposites for kanamycin detection","volume":"139","author":"Sun","year":"2014","journal-title":"Analyst"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"16281","DOI":"10.3390\/s150716281","article-title":"Nucleic acid aptamers: An emerging tool for biotechnology and biomedical sensing","volume":"15","author":"Ku","year":"2015","journal-title":"Sensors"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kumar Kulabhusan, P., Hussain, B., and Y\u00fcce, M. 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