{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T15:51:36Z","timestamp":1770997896236,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21864009"],"award-info":[{"award-number":["21864009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2018YFB1502103, 2018YFB1502105"],"award-info":[{"award-number":["2018YFB1502103, 2018YFB1502105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Bagui Scholar Foundation, Guangxi Collaborative Innovation Centre of Structure and Property for New Energy and Materials","award":["2012GXNSFGA06002"],"award-info":[{"award-number":["2012GXNSFGA06002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A single-molecule detection method was developed for nucleic acids based on mass spectrometry counting single liposome particles. Before the appearance of symptoms, a negligible amount of nucleic acids and biomarkers for the clinical diagnosis of the disease were already present. However, it is difficult to detect extremely low concentrations of nucleic acids using the current methods. Hence, the establishment of an ultra-sensitive nucleic acid detection technique is urgently needed. Herein, magnetic beads were used to capture target nucleic acids, and liposome particles were employed as mass tags for single-particle measurements. Liposomes were released from magnetic beads via photocatalytic cleavage. Hence, one DNA molecule corresponded to one liposome particle, which could be counted using mass spectrometric measurement. The ultrasensitive detection of DNA (10\u201318 M) was achieved using this method.<\/jats:p>","DOI":"10.3390\/s22041346","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T02:40:17Z","timestamp":1644547217000},"page":"1346","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3823-5353","authenticated-orcid":false,"given":"Xiangcheng","family":"Lin","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2329-4342","authenticated-orcid":false,"given":"Mengmeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"given":"Mingyue","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"given":"Juan","family":"Long","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"given":"Fang","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"given":"Fen","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4684-683X","authenticated-orcid":false,"given":"Lixian","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10469","DOI":"10.1021\/acs.chemrev.1c00241","article-title":"Nucleic acid tests for clinical translation","volume":"121","author":"Li","year":"2021","journal-title":"Chem. 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