{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T13:36:00Z","timestamp":1774445760091,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T00:00:00Z","timestamp":1645660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shenzhen Science and Technology Program","award":["KQTD20190929173954826"],"award-info":[{"award-number":["KQTD20190929173954826"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We used surface-enhanced Raman spectroscopy (SERS) for the rapid and sensitive detection and quantification of caffeine in solution. Such a technique incorporated into a portable device is finding wide applications in trace chemical analysis in various fields, including law enforcement, medicine, environmental monitoring, and food quality control. To realize such applications, we are currently developing portable and handheld trace chemical analyzers based on SERS, which are integrated with a sensor embedded with activated gold nanoparticles in a porous glass matrix. In this study, we used this gold SERS-active substrate to measure aqueous solutions of the drug caffeine as a test chemical to benchmark sensor performance by defining sensitivity (lowest measured concentration (LMC) and estimated limit of detection (LOD)), determining concentration dependence and quantification capabilities by constructing calibration curves; by evaluating the effects of pH values of 3, 7, and 11; and by examining the reproducibility of the SERS measurements. The results demonstrate that the SERS sensor is sensitive, with caffeine detected at an LMC of 50 parts per billion (ppb) with an LOD of 0.63 ppb. The results further show that the sensor is very stable and can be used to make reproducible measurements, even under extremely acidic to basic pH conditions. Vibrational assignments of all observed SERS peaks are made and reported for the first time for caffeine on a gold substrate.<\/jats:p>","DOI":"10.3390\/s22051778","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T21:11:07Z","timestamp":1645737067000},"page":"1778","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Part-Per-Billion Level Chemical Sensing with a Gold-Based SERS-Active Substrate"],"prefix":"10.3390","volume":"22","author":[{"given":"Tingting","family":"Zhang","sequence":"first","affiliation":[{"name":"Micro Optical Instruments, Shenzhen 518118, China"}]},{"given":"Liyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Micro Optical Instruments, Shenzhen 518118, China"}]},{"given":"Junchang","family":"Pei","sequence":"additional","affiliation":[{"name":"Institute of Criminal Science and Technology, Taizhou Public Security Bureau, Taizhou 225300, China"}]},{"given":"Xuefeng","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Criminal Science and Technology, Taizhou Public Security Bureau, Taizhou 225300, China"}]},{"given":"Haowen","family":"Li","sequence":"additional","affiliation":[{"name":"Micro Optical Instruments, Shenzhen 518118, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5143-291X","authenticated-orcid":false,"given":"Frank","family":"Inscore","sequence":"additional","affiliation":[{"name":"Micro Optical Instruments, Shenzhen 518118, China"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Enderlein, J., Keller, R.A., and Zander, C. 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