{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:23:42Z","timestamp":1782977022575,"version":"3.54.5"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Currently, much research is devoted to the study of biological objects using atomic force microscopy (AFM). This method\u2019s resolution is superior to the other non-scanning techniques. Our study aims to further emphasize some of the advantages of using AFM as a clinical screening tool. The study focused on red blood cells exposed to various physical and chemical factors, namely hemin, zinc ions, and long-term storage. AFM was used to investigate the morphological, nanostructural, cytoskeletal, and mechanical properties of red blood cells (RBCs). Based on experimental data, a set of important biomarkers determining the status of blood cells have been identified.<\/jats:p>","DOI":"10.3390\/s22052055","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T01:50:53Z","timestamp":1646790653000},"page":"2055","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Investigation of Red Blood Cells by Atomic Force Microscopy"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8425-0845","authenticated-orcid":false,"given":"Viktoria","family":"Sergunova","sequence":"first","affiliation":[{"name":"Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislav","family":"Leesment","sequence":"additional","affiliation":[{"name":"NT-MDT Spectrum Instruments, Proezd 4922, 4\/3 Zelenograd, 124460 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Department of Medical and Biological Physics, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4693-5624","authenticated-orcid":false,"given":"Vladimir","family":"Inozemtsev","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. 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Burdenko Main Military Clinical Hospital\u201d of the Ministry of Defense of the Russian Federation, Hospital Sq., Build. 3, 105094 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyacheslav","family":"Polyakov","sequence":"additional","affiliation":[{"name":"NT-MDT Spectrum Instruments, Proezd 4922, 4\/3 Zelenograd, 124460 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9962-6315","authenticated-orcid":false,"given":"Ekaterina","family":"Sherstyukova","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. 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