{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:46:48Z","timestamp":1761864408644,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Laboratory of NBC Protection for Civilian","award":["SKLNBC2020-03"],"award-info":[{"award-number":["SKLNBC2020-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the open reading frames 1ab (ORF1ab) target gene by a electrochemiluminescence (ECL) biosensor based on dual-probe hybridization through the use of a detection model of \u201cmagnetic capture probes\u2014targeted nucleic acids\u2014Ru(bpy)32+ labeled signal probes\u201d. The detection model used magnetic particles coupled with a biotin-labeled complementary nucleic acid sequence of the SARS-CoV-2 ORF1ab target gene as the magnetic capture probes and Ru(bpy)32+ labeled amino modified another complementary nucleic acid sequence as the signal probes, which combined the advantages of the highly specific dual-probe hybridization and highly sensitive ECL biosensor technology. In the range of 0.1 fM~10 \u00b5M, the method made possible rapid and sensitive detection of the ORF1ab gene of the SARS-CoV-2 within 30 min, and the limit of detection (LOD) was 0.1 fM. The method can also meet the analytical requirements for simulated samples such as saliva and urine with the definite advantages of a simple operation without nucleic acid amplification, high sensitivity, reasonable reproducibility, and anti-interference solid abilities, expounding a new way for efficient and sensitive detection of SARS-CoV-2.<\/jats:p>","DOI":"10.3390\/s22062402","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T21:48:42Z","timestamp":1647899322000},"page":"2402","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization"],"prefix":"10.3390","volume":"22","author":[{"given":"Chunying","family":"Jiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Xihui","family":"Mu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Shuai","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Zhiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Bin","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Jiang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]},{"given":"Jianjie","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1126\/science.abc6261","article-title":"Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans","volume":"369","author":"Arunachalam","year":"2020","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3892\/etm.2020.9587","article-title":"Confronting the threat of SARS-CoV-2: Realities, challenges and therapeutic strategies","volume":"21","author":"Wang","year":"2021","journal-title":"Exp. 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