{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T04:11:12Z","timestamp":1773461472185,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008003","name":"Korea Institute of Radiological and Medical Sciences","doi-asserted-by":"publisher","award":["50538-2022"],"award-info":[{"award-number":["50538-2022"]}],"id":[{"id":"10.13039\/501100008003","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korean government Ministry of Science and ICT","award":["50538-2022"],"award-info":[{"award-number":["50538-2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, we proposed an integrated microfluidic device that could demonstrate the non-contact, label-free separation of particles and cells through the combination of inertial microfluidics and acoustophoresis. The proposed device integrated two microfluidic chips which were a PDMS channel chip on top of the silicon-based acoustofluidic chip. The PDMS chip worked by prefocusing the particles\/cells through inducing the inertial force of the channel structure. The connected acoustofluidic chips separated particles based on their size through an acoustic radiation force. In the serpentine-shaped PDMS chip, particles formed two lines focusing in the channel, and a trifugal-shaped acoustofluidic chip displaced and separated particles, in which larger particles focused on the central channel and smaller ones moved to the side channels. The simultaneous fluidic works allowed high-efficiency particle separation. Using this novel acoustofluidic device with an inertial microchannel, the separation of particles and cells based on their size was presented and analyzed, and the efficiency of the device was shown. The device demonstrated excellent separation performance with a high recovery ratio (up to 96.3%), separation efficiency (up to 99%), and high volume rate (&gt;100 \u00b5L\/min). Our results showed that integrated devices could be a viable alternative to current cell separation based on their low cost, reduced sample consumption and high throughput capability.<\/jats:p>","DOI":"10.3390\/s22134709","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T23:11:19Z","timestamp":1655939479000},"page":"4709","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Inertia\u2013Acoustophoresis Hybrid Microfluidic Device for Rapid and Efficient Cell Separation"],"prefix":"10.3390","volume":"22","author":[{"given":"Uihwan","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Mechanical Design and Robot Engineering, Seoul National University of Science & Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byeolnim","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Electronic Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Korea"},{"name":"Institute of Precision Machinery Technology, Seoul National University of Science & Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4098-7634","authenticated-orcid":false,"given":"Jiyeon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Division of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul 01812, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Bio-Health Product Research Center, Inje University, 197 Inje-ro, Gimhae-si 50834, Gyeongsangnam-do, Korea"},{"name":"PCL, Inc., Rm 701, Star Valley, 99 Digital-ro 9-gil, Geumcheon-gu, Seoul 08510, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7603-2063","authenticated-orcid":false,"given":"Younghak","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Mechanical Design and Robot Engineering, Seoul National University of Science & Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea"},{"name":"Institute of Precision Machinery Technology, Seoul National University of Science & Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea"},{"name":"Department of Mechanical System Design Engineering, Seoul National University of Science & Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1007\/s11517-010-0611-4","article-title":"Microfluidics for Cell Separation","volume":"48","author":"Bhagat","year":"2010","journal-title":"Med. 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