{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T21:01:34Z","timestamp":1786222894986,"version":"build-2736575974"},"reference-count":33,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry for Education, University and Research","award":["MIUR, 2018-2022"],"award-info":[{"award-number":["MIUR, 2018-2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, a novel platform for lab-in-fiber-based biosensors is studied. Hollow-core tube lattice fibers (HC-TLFs) are proposed as a label-free biosensor for the detection of DNA molecules. The particular light-guiding mechanism makes them a highly sensitive tool. Their transmission spectrum is featured by alternations of high and low transmittance at wavelength regions whose values depend on the thickness of the microstructured web composing the cladding around the hollow core. In order to achieve DNA detection by using these fibers, an internal chemical functionalization process of the fiber has been performed in five steps in order to link specific peptide nucleic acid (PNA) probes, then the functionalized fiber was used for a three-step assay. When a solution containing a particular DNA sequence is made to flow through the HC of the TLF in an \u2018optofluidic\u2019 format, a bio-layer is formed on the cladding surfaces causing a red-shift of the fiber transmission spectrum. By comparing the fiber transmission spectra before and after the flowing it is possible to identify the eventual formation of the layer and, therefore, the presence or not of a particular DNA sequence in the solution.<\/jats:p>","DOI":"10.3390\/s22145144","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:06:21Z","timestamp":1657497981000},"page":"5144","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Hollow-Core Fiber-Based Biosensor: A Platform for Lab-in-Fiber Optical Biosensors for DNA Detection"],"prefix":"10.3390","volume":"22","author":[{"given":"Foroogh","family":"Khozeymeh","sequence":"first","affiliation":[{"name":"Department of Engineering and Architecture, University of Parma, Parco Area delle Scienze 181\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7909-6038","authenticated-orcid":false,"given":"Federico","family":"Melli","sequence":"additional","affiliation":[{"name":"Department of Engineering \u201cEnzo Ferrari\u201d, University of Modena and Reggio Emilia, Via Vivarelli 10, 41124 Modena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3496-3812","authenticated-orcid":false,"given":"Sabrina","family":"Capodaglio","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability (SCVSA), University of Parma, Parco Area delle Scienze 17\/a, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-0923","authenticated-orcid":false,"given":"Roberto","family":"Corradini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability (SCVSA), University of Parma, Parco Area delle Scienze 17\/a, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fetah","family":"Benabid","sequence":"additional","affiliation":[{"name":"CNRS UMR 7252, XLIM Research Institute, University of Limoges, 87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3724-2498","authenticated-orcid":false,"given":"Luca","family":"Vincetti","sequence":"additional","affiliation":[{"name":"Department of Engineering \u201cEnzo Ferrari\u201d, University of Modena and Reggio Emilia, Via Vivarelli 10, 41124 Modena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1922-0559","authenticated-orcid":false,"given":"Annamaria","family":"Cucinotta","sequence":"additional","affiliation":[{"name":"Department of Engineering and Architecture, University of Parma, Parco Area delle Scienze 181\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.1021\/ja044087q","article-title":"Detection of a biomarker for Alzheimer\u2019s disease from synthetic and clinical samples using a nanoscale optical biosensor","volume":"127","author":"Haes","year":"2005","journal-title":"J. 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