{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T04:17:12Z","timestamp":1771906632332,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T00:00:00Z","timestamp":1659139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772068"],"award-info":[{"award-number":["31772068"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019ZBXC090"],"award-info":[{"award-number":["2019ZBXC090"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LYXZ10"],"award-info":[{"award-number":["LYXZ10"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zibo-Sdut Integration Development Project","award":["31772068"],"award-info":[{"award-number":["31772068"]}]},{"name":"Zibo-Sdut Integration Development Project","award":["2019ZBXC090"],"award-info":[{"award-number":["2019ZBXC090"]}]},{"name":"Zibo-Sdut Integration Development Project","award":["LYXZ10"],"award-info":[{"award-number":["LYXZ10"]}]},{"name":"Shandong &amp; Chongqing Science and Technology Cooperation Project","award":["31772068"],"award-info":[{"award-number":["31772068"]}]},{"name":"Shandong &amp; Chongqing Science and Technology Cooperation Project","award":["2019ZBXC090"],"award-info":[{"award-number":["2019ZBXC090"]}]},{"name":"Shandong &amp; Chongqing Science and Technology Cooperation Project","award":["LYXZ10"],"award-info":[{"award-number":["LYXZ10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, we reported a rapid and sensitive fluorescence assay in homogenous solution for detecting organophosphorus pesticides by using tetramethylrhodamine (TAMRA)-labeled aptamer and its complementary DNA (cDNA) with extended guanine (G) bases. The hybridization of cDNA and aptamer drew TAMRA close to repeated G bases, then the fluorescence of TAMRA was quenched by G bases due to the photoinduced electron transfer (PET). Upon introducing the pesticide target, the aptamer bound to pesticide instead of cDNA because of the competition between pesticide and cDNA. Thus, the TAMRA departed from G bases, resulting in fluorescence recovery of TAMRA. Under optimal conditions, the limits of detection for phorate, profenofos, isocarbophos, and omethoate were 0.333, 0.167, 0.267, and 0.333 \u00b5g\/L, respectively. The method was also used in the analysis of profenofos in vegetables. Our fluorescence design was simple, rapid, and highly sensitive, which provided a means for monitoring the safety of agricultural products.<\/jats:p>","DOI":"10.3390\/s22155712","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T23:49:27Z","timestamp":1659397767000},"page":"5712","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Fluorescence Assay for Detecting Four Organophosphorus Pesticides Using Fluorescently Labeled Aptamer"],"prefix":"10.3390","volume":"22","author":[{"given":"He","family":"Li","sequence":"first","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingcheng","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyuan","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengjiao","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yemin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1021\/acs.jafc.7b05119","article-title":"Quantum dots applied to methodology on detection of pesticide and veterinary drug residues","volume":"66","author":"Zhou","year":"2018","journal-title":"J. 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