{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T13:35:25Z","timestamp":1778852125323,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:00:00Z","timestamp":1659916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Medical Device Development Fund","award":["KMDF_PR_20200901_0023"],"award-info":[{"award-number":["KMDF_PR_20200901_0023"]}]},{"name":"Korea Medical Device Development Fund","award":["1711137928"],"award-info":[{"award-number":["1711137928"]}]},{"name":"Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health &amp; Welfare, the Ministry of Food and Drug Safety)","award":["KMDF_PR_20200901_0023"],"award-info":[{"award-number":["KMDF_PR_20200901_0023"]}]},{"name":"Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health &amp; Welfare, the Ministry of Food and Drug Safety)","award":["1711137928"],"award-info":[{"award-number":["1711137928"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Hydrogen sulfide (H2S) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify H2S produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial H2S utilizing silver\/Nafion\/polyvinylpyrrolidone membrane and a 96-well microplate. This H2S-sensing paper showed a good sensitivity (8.27 blue channel intensity\/\u03bcM H2S, R2 = 0.9996), which was higher than that of lead acetate paper (6.05 blue channel intensity\/\u03bcM H2S, R2 = 0.9959). We analyzed the difference in H2S concentration released from four kinds of oral bacteria (Eikenella corrodens, Streptococcus sobrinus, Streptococcus mutans, and Lactobacillus casei). Finally, the H2S level in Eikenella corrodens while varying the concentration of cysteine and treatment time was quantified. This paper-based colorimetric assay can be utilized as a simple and effective tool for in vitro screening of H2S-producing ability of many bacteria as well as salivary H2S analysis.<\/jats:p>","DOI":"10.3390\/s22155928","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T04:16:55Z","timestamp":1660018615000},"page":"5928","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Simple and Sensitive Detection of Bacterial Hydrogen Sulfide Production Using a Paper-Based Colorimetric Assay"],"prefix":"10.3390","volume":"22","author":[{"given":"Byung-Ki","family":"Ahn","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Jin","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-0411","authenticated-orcid":false,"given":"Yeon-Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orofacial Pain and Oral Medicine, Kyung Hee University Dental Hospital, Kyung Hee University School of Dentistry, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-7796","authenticated-orcid":false,"given":"Gi-Ja","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea"},{"name":"Department of Medical Engineering, Kyung Hee University Graduate School, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2017225118","DOI":"10.1073\/pnas.2017225118","article-title":"Hydrogen sulfide is neuroprotective in Alzheimer\u2019s disease by sulfhydrating GSK3\u03b2 and inhibiting Tau hyperphosphorylation","volume":"118","author":"Giovinazzo","year":"2021","journal-title":"Proc. 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