{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:36:27Z","timestamp":1779914187892,"version":"3.53.1"},"reference-count":47,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"Sanford Health-North Dakota State University Collaborative Seed Grant Program and National Science Foundation","doi-asserted-by":"publisher","award":["1941748"],"award-info":[{"award-number":["1941748"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"Sanford Health-North Dakota State University Collaborative Seed Grant Program and National Science Foundation","doi-asserted-by":"publisher","award":["1P20GM109024"],"award-info":[{"award-number":["1P20GM109024"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of General Medical Sciences of the National Institutes of Health","doi-asserted-by":"publisher","award":["1941748"],"award-info":[{"award-number":["1941748"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of General Medical Sciences of the National Institutes of Health","doi-asserted-by":"publisher","award":["1P20GM109024"],"award-info":[{"award-number":["1P20GM109024"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Weight loss through dietary and exercise intervention is commonly prescribed but is not effective for all individuals. Recent studies have demonstrated that circulating microRNA (miR) biomarkers could potentially be used to identify individuals who will likely lose weight through diet and exercise and attain a healthy body weight. However, accurate detection of miRs in clinical samples is difficult, error-prone, and expensive. To address this issue, we recently developed iLluminate\u2014a low-cost and highly sensitive miR sensor suitable for point-of-care testing. To investigate if miR testing and iLluminate can be used in real-world obesity applications, we developed a pilot diet and exercise intervention and utilized iLluminate to evaluate miR biomarkers. We evaluated the expression of miRs-140, -935, -let-7b, and -99a, which are biomarkers for fat loss, energy metabolism, and adipogenic differentiation. Responders lost more total mass, tissue mass, and fat mass than non-responders. miRs-140, -935, -let-7b, and -99a, collectively accounted for 6.9% and 8.8% of the explained variability in fat and lean mass, respectively. At the level of the individual coefficients, miRs-140 and -935 were significantly associated with fat loss. Collectively, miRs-140 and -935 provide an additional degree of predictive capability in body mass and fat mass alternations.<\/jats:p>","DOI":"10.3390\/s22186758","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T04:18:32Z","timestamp":1662610712000},"page":"6758","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Miniaturized MicroRNA Sensor Identifies Targets Associated with Weight Loss in a Diet and Exercise Intervention among Healthy Overweight Individuals"],"prefix":"10.3390","volume":"22","author":[{"given":"Vidura","family":"Jayasooriya","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1577-3167","authenticated-orcid":false,"given":"Nathaniel","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Health, Nutrition, and Exercise Sciences, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Health, Nutrition, and Exercise Sciences, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8925-997X","authenticated-orcid":false,"given":"Christopher","family":"Kotarsky","sequence":"additional","affiliation":[{"name":"Department of Health, Nutrition, and Exercise Sciences, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizzy","family":"Jepng\u2019etich","sequence":"additional","affiliation":[{"name":"Department of Statistics, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1095-345X","authenticated-orcid":false,"given":"Daniel","family":"Friesner","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2555-390X","authenticated-orcid":false,"given":"Sherri","family":"Stastny","sequence":"additional","affiliation":[{"name":"Department of Health, Nutrition, and Exercise Sciences, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1586-5392","authenticated-orcid":false,"given":"Kyle J.","family":"Hackney","sequence":"additional","affiliation":[{"name":"Department of Health, Nutrition, and Exercise Sciences, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharmakeerthi","family":"Nawarathna","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND 58108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1001\/jamainternmed.2015.2405","article-title":"Prevalence of Overweight and Obesity in the United States, 2007\u20132012 Computed Tomography Radiation Dose in Patients with Suspected Urolithiasis Computed tomography (CT) for the evaluation of suspected","volume":"175","author":"Yang","year":"2015","journal-title":"JAMA Intern. 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