{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:09:48Z","timestamp":1760231388879,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["IRG105878","120321","121891","145848"],"award-info":[{"award-number":["IRG105878","120321","121891","145848"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Pretoria","award":["IRG105878","120321","121891","145848"],"award-info":[{"award-number":["IRG105878","120321","121891","145848"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The adverse health effects of the presence of lead in wastewater streams are well documented, with conventional methods of lead recovery and removal suffering from disadvantages such as high energy costs, the production of toxic sludge, and low lead selectivity. Klebsiella pneumoniae and Paraclostridium bifermentans have been identified as potential lead-precipitating species for use in a lead recovery bioreactor. Electrical impedance spectroscopy (EIS) on a low-cost device is used to determine the potential for the probe-free and label-free monitoring of cell growth in a bioreactor containing these bacteria. A complex polynomial is fit for several reactive equivalent circuit components. A direct correlation is found between the extracted supercapacitance and the plated colony-forming unit count during the exponential growth phase, and a qualitative correlation is found between all elements of the measured reactance outside the exponential growth phase. Strong evidence is found that Pb(II) ions act as an anaerobic respiration co-substrate for both cells observed, with changes in plated count qualitatively mirrored in the Pb(II) concentration. Guidance is given on the implementation of EIS devices for continuous impedance monitoring.<\/jats:p>","DOI":"10.3390\/s22187045","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T04:49:22Z","timestamp":1663562962000},"page":"7045","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non-Destructive Impedance Monitoring of Bacterial Metabolic Activity towards Continuous Lead Biorecovery"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7943-8827","authenticated-orcid":false,"given":"George","family":"Andrews","sequence":"first","affiliation":[{"name":"Carl and Emily Fuchs Institute for Microelectronics (CEFIM), University of Pretoria, Pretoria 0002, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5729-6656","authenticated-orcid":false,"given":"Olga","family":"Neveling","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology, University of Pretoria, Pretoria 0002, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7344-3361","authenticated-orcid":false,"given":"Dirk Johannes","family":"De Beer","sequence":"additional","affiliation":[{"name":"Carl and Emily Fuchs Institute for Microelectronics (CEFIM), University of Pretoria, Pretoria 0002, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6255-862X","authenticated-orcid":false,"given":"Evans M. 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