{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T19:42:00Z","timestamp":1788550920079,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, a novel non-enzymatic modified glassy carbon (GC) sensor, of the (GC-Agpaste)-catalytic proline-assisted LDH type, for H2O2 determination was fabricated, studied, characterized and employed to determine the hydrogen peroxide content in healthy and diabetic human urine. LDH (whose composition can be schematized as [ZnIIAlIII (OH)2]+ NO3\u2212\u00b7nH2O) is glued to glassy carbon by means of silver paste, while proline, which increases the catalytic properties of LDH, is used free in solution in the phosphate buffer. A voltametric survey was first conducted to ascertain the positive effect induced by the presence of proline, i.e., the increase of sensor sensitivity. Then a deep study of the new three-electrode amperometric proline-assisted LDH sensor, whose working electrode was of the same type as the one used to perform the cyclic voltammetry, was carried out, working at first in static air, then in a nitrogen atmosphere. Possible interferences from various substances, both oxidants and antioxidants, were also investigated. Lastly, the new amperometric sensor was successfully used to determine the H2O2 level in human urine from both healthy and diabetic subjects. The effect of proline in enhancing the properties of the sensor system was also investigated. The limit of detection (LOD) of the new catalytic sensor was of the order of 0.15 mmol L\u22121, working in air, and of 0.05 \u00b5mol L\u22121, working in nitrogen atmosphere.<\/jats:p>","DOI":"10.3390\/s22197159","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T23:07:55Z","timestamp":1663888075000},"page":"7159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Novel Electrochemical Sensors Based on L-Proline Assisted LDH for H2O2 Determination in Healthy and Diabetic Urine"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3292-1053","authenticated-orcid":false,"given":"Mauro","family":"Tomassetti","sequence":"first","affiliation":[{"name":"Department of Electronic Engineering, University of Rome \u2018\u2018Tor Vergata\u2019\u2019, Viale del Politecnico 1, 00133 Rome, Italy"},{"name":"Department of Chemistry, University of Rome \u201cLa Sapienza\u201d, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Pezzilli","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Rome \u2018\u2018Tor Vergata\u2019\u2019, Viale del Politecnico 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2916-5883","authenticated-orcid":false,"given":"Giuseppe","family":"Prestopino","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Rome \u2018\u2018Tor Vergata\u2019\u2019, Viale del Politecnico 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0543-4348","authenticated-orcid":false,"given":"Corrado","family":"Di Natale","sequence":"additional","affiliation":[{"name":"Department of Electronic Engineering, University of Rome \u2018\u2018Tor Vergata\u2019\u2019, Viale del Politecnico 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier Gianni","family":"Medaglia","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Rome \u2018\u2018Tor Vergata\u2019\u2019, Viale del Politecnico 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","unstructured":"United States Pharmacopeia (2022). Hydrogen Peroxide Concentrate, United States Pharmacopeia (USP-NF). USP Monographs."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s11244-005-2529-x","article-title":"Wet Hydrogen Peroxide Catalytic Oxidation (WHPCO) of Organic Waste in Agro-Food and Industrial Streams","volume":"33","author":"Perathoner","year":"2005","journal-title":"Top. Catal."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.foodchem.2009.10.024","article-title":"Fluorometric Determination of Hydrogen Peroxide in Milk by Using a Fenton Reaction System","volume":"120","author":"Abbas","year":"2010","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.talanta.2018.10.068","article-title":"Use of Zein Microspheres to Anchor Carbon Black and Hemoglobin in Electrochemical Biosensors to Detect Hydrogen Peroxide in Cosmetic Products, Food and Biological Fluids","volume":"194","author":"Pereira","year":"2019","journal-title":"Talanta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8453","DOI":"10.1021\/ac901300b","article-title":"Real-Time Monitoring of Oxidative Burst from Single Plant Protoplasts Using Microelectrochemical Sensors Modified by Platinum Nanoparticles","volume":"81","author":"Ai","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13648","DOI":"10.1021\/am503110s","article-title":"Immobilizing Gold Nanoparticles in Mesoporous Silica Covered Reduced Graphene Oxide: A Hybrid Material for Cancer Cell Detection through Hydrogen Peroxide Sensing","volume":"6","author":"Maji","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.snb.2015.01.064","article-title":"Detection of Cellular H2O2 in Living Cells Based on Horseradish Peroxidase at the Interface of Au Nanoparticles Decorated Graphene Oxide","volume":"211","author":"Yu","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"22563","DOI":"10.1021\/acsami.6b05561","article-title":"Pd Nanoparticles Decorated N-Doped Graphene Quantum Dots@N-Doped Carbon Hollow Nanospheres with High Electrochemical Sensing Performance in Cancer Detection","volume":"8","author":"Xi","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15392","DOI":"10.1021\/ja0441716","article-title":"A Selective, Cell-Permeable Optical Probe for Hydrogen Peroxide in Living Cells","volume":"126","author":"Chang","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.snb.2017.03.012","article-title":"Determination of Hydrogen Peroxide and Triacetone Triperoxide (TATP) with a Silver Nanoparticles\u2014Based Turn-on Colorimetric Sensor","volume":"247","author":"Durmazel","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9816","DOI":"10.1039\/C4AY02339K","article-title":"Colorimetric Determination of Hydrogen Peroxide by Morphological Decomposition of Silver Nanoprisms Coupled with Chromaticity Analysis","volume":"6","author":"Nitinaivinij","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"25716","DOI":"10.3390\/s151025716","article-title":"A Spectrometric Method for Hydrogen Peroxide Concentration Measurement with a Reusable and Cost-Efficient Sensor","volume":"15","author":"Hsu","year":"2015","journal-title":"Sensors"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0039-9140(99)00277-5","article-title":"Stopped-Flow Spectrophotometric Determination of Hydrogen Peroxide with Hemoglobin as Catalyst","volume":"51","author":"Zhang","year":"2000","journal-title":"Talanta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/j.saa.2013.08.048","article-title":"Spectrophotometric Determination of Hydrogen Peroxide with Osmium(VIII) and m-Carboxyphenylfluorone","volume":"117","author":"Hoshino","year":"2014","journal-title":"Spectrochim. Acta. A Mol. Biomol. Spectrosc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0003-2670(00)85587-7","article-title":"Spectrophotometric Determination of Hydrogen Peroxide and Organic Hydropheroxides at Low Concentrations in Aqueous Solution","volume":"155","author":"Frew","year":"1983","journal-title":"Anal. Chim. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/S0039-9140(97)00012-X","article-title":"Spectrophotometric Determination of Hydrogen Peroxide by Using the Cleavage of Eriochrome Black T in the Presence of Peroxidase","volume":"44","author":"Zhu","year":"1997","journal-title":"Talanta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.talanta.2017.01.066","article-title":"Silver Nanoclusters-Catalyzed Luminol Chemiluminescence for Hydrogen Peroxide and Uric Acid Detection","volume":"166","author":"Sheng","year":"2017","journal-title":"Talanta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1039\/C1AN15738H","article-title":"Recent Advances in Electrochemical Sensing for Hydrogen Peroxide: A Review","volume":"137","author":"Chen","year":"2012","journal-title":"Analyst"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"G82","DOI":"10.1149\/2.1061908jes","article-title":"Review\u2014Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy","volume":"166","author":"Gulaboski","year":"2019","journal-title":"J. Electrochem. Soc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.electacta.2017.12.084","article-title":"3D Ordered Macroporous SmCoO3 Perovskite for Highly Active and Selective Hydrogen Peroxide Detection","volume":"260","author":"He","year":"2018","journal-title":"Electrochim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1039\/C0AN00825G","article-title":"Electrochemical Detection of Extracellular Hydrogen Peroxide Released from RAW 264.7 Murine Macrophage Cells Based on Horseradish Peroxidase\u2013Hydroxyapatite Nanohybrids","volume":"136","author":"Li","year":"2011","journal-title":"Analyst"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1016\/j.bios.2011.03.018","article-title":"Electrochemical Measurement of the Flux of Hydrogen Peroxide Releasing from RAW 264.7 Macrophage Cells Based on Enzyme-Attapulgite Clay Nanohybrids","volume":"26","author":"Wu","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13603","DOI":"10.1021\/acs.langmuir.8b02005","article-title":"In Situ Synthesis of Silver Nanospheres, Nanocubes, and Nanowires over Boron-Doped Graphene Sheets for Surface-Enhanced Raman Scattering Application and Enzyme-Free Detection of Hydrogen Peroxide","volume":"34","author":"Nair","year":"2018","journal-title":"Langmuir"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.carbpol.2017.05.021","article-title":"Photochemical Synthesis of Silver Nanoparticles on Chitosans\/Montmorillonite Nanocomposite Films and Antibacterial Activity","volume":"171","author":"Gabriel","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.snb.2017.10.035","article-title":"An Inkjet Printed Ag Electrode Fabricated on Plastic Substrate with a Chemical Sintering Approach for the Electrochemical Sensing of Hydrogen Peroxide","volume":"256","author":"Shi","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.apsusc.2017.12.235","article-title":"Investigate the Electrical and Thermal Properties of the Low Temperature Resistant Silver Nanowire Fabricated by Two-Beam Laser Technique","volume":"439","author":"He","year":"2018","journal-title":"Appl. Surf. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.snb.2016.07.016","article-title":"Size Controllable Preparation of Gold Nanoparticles Loading on Graphene Sheets@cerium Oxide Nanocomposites Modified Gold Electrode for Nonenzymatic Hydrogen Peroxide Detection","volume":"238","author":"Yang","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.bios.2015.01.017","article-title":"Real-Time Electrochemical Detection of Hydrogen Peroxide Secretion in Live Cells by Pt Nanoparticles Decorated Graphene\u2013Carbon Nanotube Hybrid Paper Electrode","volume":"68","author":"Sun","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6557","DOI":"10.1002\/anie.200501272","article-title":"Tungsten Carbide Microspheres as a Noble-Metal-Economic Electrocatalyst for Methanol Oxidation","volume":"44","author":"Ganesan","year":"2005","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7040","DOI":"10.1039\/C4CS00160E","article-title":"Catalytic Applications of Layered Double Hydroxides: Recent Advances and Perspectives","volume":"43","author":"Fan","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5291","DOI":"10.1039\/C5CS00268K","article-title":"Supported Catalysts Based on Layered Double Hydroxides for Catalytic Oxidation and Hydrogenation: General Functionality and Promising Application Prospects","volume":"44","author":"Feng","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.aca.2015.09.053","article-title":"Real-Time Tracking of Hydrogen Peroxide Secreted by Live Cells Using MnO2 Nanoparticles Intercalated Layered Doubled Hydroxide Nanohybrids","volume":"898","author":"Asif","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Arrabito, G., Pezzilli, R., Prestopino, G., and Medaglia, P.G. (2020). Layered Double Hydroxides in Bioinspired Nanotechnology. Crystals, 10.","DOI":"10.3390\/cryst10070602"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"106700","DOI":"10.1016\/j.microc.2021.106700","article-title":"Fabrication and Characterization of a Layered Double Hydroxide Based Catalase Biosensor and a Catalytic Sensor for Hydrogen Peroxide Determination","volume":"170","author":"Tomassetti","year":"2021","journal-title":"Microchem. J."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tomassetti, M., Pezzilli, R., Prestopino, G., Di Biagio, F., Di Natale, C., and Medaglia, P.G. (2021). A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H2O2 Determination in Highly Diluted Real Matrices: Milk and Cosmetics. Processes, 9.","DOI":"10.3390\/pr9111878"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jelechem.2016.01.042","article-title":"Synthesis of Hexagonal CoAl-Layered Double Hydroxide Nanoshales\/Carbon Nanotubes Composite for the Non-Enzymatic Detection of Hydrogen Peroxide","volume":"768","author":"Heli","year":"2016","journal-title":"J. Electroanal. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.bios.2017.05.057","article-title":"Core-Shell Iron Oxide-Layered Double Hydroxide: High Electrochemical Sensing Performance of H 2 O 2 Biomarker in Live Cancer Cells with Plasma Therapeutics","volume":"97","author":"Asif","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jelechem.2018.11.050","article-title":"One-Step Voltammetric Deposition of l-Proline Assisted Silver Nanoparticles Modified Glassy Carbon Electrode for Electrochemical Detection of Hydrogen Peroxide","volume":"833","author":"Zhao","year":"2019","journal-title":"J. Electroanal. Chem."},{"key":"ref_39","first-page":"365","article-title":"Poly (l-Proline) Modified Carbon Paste Electrode as the Voltammetric Sensor for the Detection of Estriol and Its Simultaneous Determination with Folic and Ascorbic Acid","volume":"2","author":"Charithra","year":"2019","journal-title":"Mater. Sci. Energy Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1007\/s00604-017-2428-4","article-title":"Amperometric Sensing of Hydrogen Peroxide via an ITO Electrode Modified with Gold Nanoparticles Electrodeposited on a CoMn-Layered Double Hydroxide","volume":"184","author":"Xu","year":"2017","journal-title":"Microchim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.snb.2012.09.056","article-title":"Electrochemical Sensing Platform of Natural Estrogens Based on the Poly(l-Proline)-Ordered Mesoporous Carbon Composite Modified Glassy Carbon Electrode","volume":"187","author":"Luo","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5197","DOI":"10.1039\/c0cc00313a","article-title":"Layered Double Hydroxide Films: Synthesis, Properties and Applications","volume":"46","author":"Guo","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1021\/cm980523u","article-title":"Layered Double Hydroxide Stability. 1. Relative Stabilities of Layered Double Hydroxides and Their Simple Counterparts","volume":"11","author":"Boclair","year":"1999","journal-title":"Chem. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1021\/la940703s","article-title":"Synthesis and Characterization of Sol\u2212Gel Hydrotalcites. Structure and Texture","volume":"12","author":"Lopez","year":"1996","journal-title":"Langmuir"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"100631","DOI":"10.1016\/j.pmatsci.2019.100631","article-title":"Layered Intercalation Compounds: Mechanisms, New Methodologies, and Advanced Applications","volume":"109","author":"Laipan","year":"2020","journal-title":"Prog. Mater. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/S1572-4352(05)01039-1","article-title":"Layered Double Hydroxides","volume":"Volume 1","author":"Bergaya","year":"2006","journal-title":"Handbook of Clay Science"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"11498","DOI":"10.1038\/s41598-019-48012-8","article-title":"Emerging Switchable Ultraviolet Photoluminescence in Dehydrated Zn\/Al Layered Double Hydroxide Nanoplatelets","volume":"9","author":"Prestopino","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.jallcom.2016.09.125","article-title":"A Phenomenological Investigation on Chlorine Intercalated Layered Double Hydroxides Used as Room Temperature Gas Sensors","volume":"692","author":"Polese","year":"2017","journal-title":"J. Alloys Compd."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1039\/as9730300059","article-title":"Selective Ion-Sensitive Electrodes","volume":"3","author":"Moody","year":"1973","journal-title":"Sel. Annu. Rev. Anal. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/B978-0-08-028434-7.50006-7","article-title":"Uses of Known Addition, Gran\u2019s Plots and the Related Methods with Ion-Selective Electrodes","volume":"Volume 2","author":"Mascini","year":"1981","journal-title":"Ion-Selective Electrode Reviews"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jpowsour.2012.09.101","article-title":"The Application of Zn\u2013Al-Hydrotalcite as a Novel Anodic Material for Ni\u2013Zn Secondary Cells","volume":"224","author":"Fan","year":"2013","journal-title":"J. Power Sources"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.bioelechem.2018.04.009","article-title":"Hydrogen Peroxide Biosensor Based on Chitosan\/2D Layered Double Hydroxide Composite for the Determination of H2O2","volume":"123","author":"Yuan","year":"2018","journal-title":"Bioelectrochemistry"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ijpharm.2003.09.043","article-title":"Intercalation of Amino Acids and Peptides into Mg\u2013Al Layered Double Hydroxide by Reconstruction Method","volume":"269","author":"Nakayama","year":"2004","journal-title":"Int. J. Pharm."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Amine, A., and Mohammadi, H. (2018). Amperometry. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, Elsevier.","DOI":"10.1016\/B978-0-12-409547-2.14204-0"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00604-007-0840-x","article-title":"Direct Electrochemistry and Electrocatalysis of Hemoglobin in a Multilayer {nanogold\/PDDA}n Inorganic\u2013Organic Hybrid Film","volume":"160","author":"Tang","year":"2008","journal-title":"Microchim. Acta"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"8237","DOI":"10.3390\/s8128237","article-title":"Eliminating the Interference of Oxygen for Sensing Hydrogen Peroxide with the Polyaniline Modified Electrode","volume":"8","author":"Gu","year":"2008","journal-title":"Sensors"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.electacta.2012.12.038","article-title":"Electrodepositing Ag Nanodendrites on Layered Double Hydroxides Modified Glassy Carbon Electrode: Novel Hierarchical Structure for Hydrogen Peroxide Detection","volume":"90","author":"Yang","year":"2013","journal-title":"Electrochim. Acta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s00604-010-0356-7","article-title":"Electrodeposition of Silver Nanoparticles on a Zinc Oxide Film: Improvement of Amperometric Sensing Sensitivity and Stability for Hydrogen Peroxide Determination","volume":"169","author":"Wang","year":"2010","journal-title":"Microchim. Acta"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jelechem.2015.04.004","article-title":"Novel Nonenzymatic Hydrogen Peroxide Sensor Based on Fe3O4\/PPy\/Ag Nanocomposites","volume":"747","author":"Qi","year":"2015","journal-title":"J. Electroanal. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.bios.2016.02.064","article-title":"A Novel and Facile Synthesis of Carbon Quantum Dots via Salep Hydrothermal Treatment as the Silver Nanoparticles Support: Application to Electroanalytical Determination of H2O2 in Fetal Bovine Serum","volume":"81","author":"Jahanbakhshi","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Preedy, V.R., and Patel, V.B. (2014). Urinary Hydrogen Peroxide as Biomarker. General Methods in Biomarker Research and their Applications, Springer Netherlands.","DOI":"10.1007\/978-94-007-7740-8"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.cccn.2004.07.026","article-title":"Measurement of Urinary Hydrogen Peroxide by FOX-1 Method in Conjunction with Catalase in Diabetes Mellitus\u2014A Sensitive and Specific Approach","volume":"350","author":"Banerjee","year":"2004","journal-title":"Clin. Chim. Acta"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/19\/7159\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:36:48Z","timestamp":1760143008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/19\/7159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,21]]},"references-count":62,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["s22197159"],"URL":"https:\/\/doi.org\/10.3390\/s22197159","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,21]]}}}