{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:37:30Z","timestamp":1769751450300,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS Core-to-Core program","award":["20K15151"],"award-info":[{"award-number":["20K15151"]}]},{"name":"JSPS Grant-in-Aid for Scientific Research","award":["20K15151"],"award-info":[{"award-number":["20K15151"]}]},{"name":"Amada Foundation","award":["20K15151"],"award-info":[{"award-number":["20K15151"]}]},{"name":"NSG Foundation","award":["20K15151"],"award-info":[{"award-number":["20K15151"]}]},{"name":"White Rock Foundation","award":["20K15151"],"award-info":[{"award-number":["20K15151"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 \u00b5m and 5 \u00b5m, our work shows that narrowing the electrode span could greatly improve detection sensitivity. The mechanism underlying the sensitivity improvement was analyzed via numerical simulation. The small electrode gap (1 \u00b5m) allows the electric field to concentrate near the detection area, resulting in a high sensitivity for tiny particles. This finding is also verified with the mixture suspension of 1 \u00b5m and 3 \u00b5m polystyrene beads. As a result, the electrodes with 1 \u00b5m gap can detect more 1 \u00b5m beads in the suspension than electrodes with 5 \u00b5m gap. Additionally, for single yeast cells analysis, it is found that impedance cytometry with 1 \u00b5m electrodes gap can easily distinguish budding yeast cells, which cannot be realized by the impedance cytometry with 5 \u00b5m electrodes gap. All experimental results support that narrowing the electrode gap is necessary for tiny particle detection, which is an important step in the development of submicron and nanoscale impedance cytometry.<\/jats:p>","DOI":"10.3390\/s22207743","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T22:45:29Z","timestamp":1665614729000},"page":"7743","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span"],"prefix":"10.3390","volume":"22","author":[{"given":"Xun","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma 630-0192, Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0477-8322","authenticated-orcid":false,"given":"Tao","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma 630-0192, Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Wei","family":"Yi","sequence":"additional","affiliation":[{"name":"Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma 630-0192, Nara, Japan"},{"name":"Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yapeng","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka, Suita 565-0871, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Lei","sequence":"additional","affiliation":[{"name":"The Institute of Technological Sciences, Wuhan University, Wuhan 430072, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"School of Engineering, Macquarie University, Sydney 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yo","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka, Suita 565-0871, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichiroh","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma 630-0192, Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0569-6740","authenticated-orcid":false,"given":"Yaxiaer","family":"Yalikun","sequence":"additional","affiliation":[{"name":"Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma 630-0192, Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Huang, X., Liu, K., Lan, T., Wang, Z., and Zhu, Z. 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