{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:30:46Z","timestamp":1766503846998,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Gingival crevicular fluid (GCF) is a site-specific exudate deriving from the epithelium lining of the gingival sulcus. GCF analysis provides a simple and noninvasive diagnostic procedure to follow-up periodontal and bone remodeling in response to diseases or mechanical stimuli such as orthodontic tooth movement (OTM). In recent years, the use of vibrational spectroscopies such as Fourier Transform Infrared and Raman microspectroscopy and Surface-Enhanced Raman spectroscopy contributed to characterizing changes in GCF during fixed orthodontic treatment. Amide I band plays a relevant role in the analysis of these changes. The aim of this study was to investigate the spectroscopy response of Amide I depending on the OTM process duration. A model based on Gaussian\u2013Lorentzian curves was used to analyze the infrared spectra, while only Lorentzian functions were used for Raman and SERS spectra. Changes induced by the OTM process in subcomponents of the Amide I band were determined and ascribed to secondary structure modification occurring in proteins. The vibrational spectroscopies allow us to efficiently monitor the effects of the orthodontic force application, thus gaining increasing attention as tools for individual patient personalization in clinical practice.<\/jats:p>","DOI":"10.3390\/s22207874","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T05:08:02Z","timestamp":1665983282000},"page":"7874","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["\u03bc-FTIR, \u03bc-Raman, and SERS Analysis of Amide I Spectral Region in Oral Biofluid Samples during Orthodontic Treatment"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6014-0588","authenticated-orcid":false,"given":"Carlo","family":"Camerlingo","sequence":"first","affiliation":[{"name":"CNR-SPIN, Consiglio Nazionale delle Ricerche, Istituto Superconduttori, Materiali Innovativi e Dispositivi, 80078 Pozzuoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4463-0779","authenticated-orcid":false,"given":"Marianna","family":"Portaccio","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della Campania \u201cL. 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Maria di Costantinopoli 16, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0291-9339","authenticated-orcid":false,"given":"Fabrizia","family":"d\u2019Apuzzo","sequence":"additional","affiliation":[{"name":"Dipartimento Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Universit\u00e0 degli Studi della Campania Luigi Vanvitelli, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7174-7596","authenticated-orcid":false,"given":"Ludovica","family":"Nucci","sequence":"additional","affiliation":[{"name":"Dipartimento Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Universit\u00e0 degli Studi della Campania Luigi Vanvitelli, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6175-7363","authenticated-orcid":false,"given":"Letizia","family":"Perillo","sequence":"additional","affiliation":[{"name":"Dipartimento Multidisciplinare di Specialit\u00e0 Medico-Chirurgiche e Odontoiatriche, Universit\u00e0 degli Studi della Campania Luigi Vanvitelli, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-5904","authenticated-orcid":false,"given":"Maria","family":"Lepore","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, Via S. Maria di Costantinopoli 16, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Camerlingo, C., Nucci, L., d\u2019Apuzzo, F., Grassia, V., Perillo, L., Portaccio, M., and Lepore, M. (2021, January 1\u201315). Amide I band analysis applied to vibrational micro-spectroscopies of gingival crevicular fluid samples for orthodontic treatment monitoring. 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