{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:26:55Z","timestamp":1760232415482,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI Grant","award":["16H01855","19H01172"],"award-info":[{"award-number":["16H01855","19H01172"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Background: Microcirculation is a vital sign that supplies oxygen and nutrients to maintain normal life activities. Sepsis typically influences the operation of microcirculation, which is recovered by the administration of medicine injection. Objective: Sepsis-induced variation and recovery of microcirculation are quantitatively detected using microcirculation images acquired by a non-contact imaging setup, which might assist the clinical diagnosis and therapy of sepsis. Methods: In this study, a non-contact imaging setup was first used to record images of microcirculation on the back of model rats. Specifically, the model rats were divided into three groups: (i) the sham group as a control group; (ii) the cecum ligation and puncture (CLP) group with sepsis; and (iii) the CLP+thrombomodulin (TM) group with sepsis and the application of TM alfa therapy. Furthermore, considering the sparsity of red blood cells (RBCs), the blood velocity is estimated by robust principal component analysis (RPCA) and U-net, and the blood vessel diameter is estimated by the contrast difference between the blood vessel and tissue. Results and Effectiveness: In the experiments, the continuous degradation of the estimated blood velocity and blood vessel diameter in the CLP group and the recovery after degradation of those in the CLP+TM group were quantitatively observed. The variation tendencies of the estimated blood velocity and blood vessel diameter in each group suggested the effects of sepsis and its corresponding therapy.<\/jats:p>","DOI":"10.3390\/s22218471","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T04:00:51Z","timestamp":1667534451000},"page":"8471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acquisition and Analysis of Microcirculation Image in Septic Model Rats"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2555-1998","authenticated-orcid":false,"given":"Chen","family":"Ye","sequence":"first","affiliation":[{"name":"Center for Frontier Medical Engineering, Chiba University, Chiba 263-8522, Japan"}]},{"given":"Mami","family":"Kawasaki","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Chiba University, Chiba 263-8522, Japan"}]},{"given":"Kazuya","family":"Nakano","sequence":"additional","affiliation":[{"name":"Faculty of Science and Technology, Seikei University, Tokyo 180-8633, Japan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6152-4487","authenticated-orcid":false,"given":"Takashi","family":"Ohnishi","sequence":"additional","affiliation":[{"name":"Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6731-909X","authenticated-orcid":false,"given":"Eizo","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan"}]},{"given":"Shigeto","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-556X","authenticated-orcid":false,"given":"Taka-Aki","family":"Nakada","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan"}]},{"given":"Hideaki","family":"Haneishi","sequence":"additional","affiliation":[{"name":"Center for Frontier Medical Engineering, Chiba University, Chiba 263-8522, Japan"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (Sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1001\/jama.2019.5358","article-title":"Effect of a recombinant human soluble thrombomodulin on mortality in patients with sepsis-associated coagulopathy","volume":"321","author":"Vincent","year":"2019","journal-title":"JAMA"},{"key":"ref_3","first-page":"477","article-title":"Effects of thrombomodulin alfa on disseminated intravascular coagulation and organ injury in cecal ligation and puncture-induced sepsis in rats","volume":"42","author":"Arioka","year":"2014","journal-title":"Jpn. 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