{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T18:23:23Z","timestamp":1778351003830,"version":"3.51.4"},"reference-count":74,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T00:00:00Z","timestamp":1668729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AMDI Inc. (Autonomous Medical Devices Incorporated)","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Centrifugal microfluidic platforms (CDs) have opened new possibilities for inexpensive point-of-care (POC) diagnostics. They are now widely used in applications requiring polymerase chain reaction steps, blood plasma separation, serial dilutions, and many other diagnostic processes. CD microfluidic devices allow a variety of complex processes to transfer onto the small disc platform that previously were carried out by individual expensive laboratory equipment requiring trained personnel. The portability, ease of operation, integration, and robustness of the CD fluidic platforms requires simple, reliable, and scalable designs to control the flow of fluids. Valves play a vital role in opening\/closing of microfluidic channels to enable a precise control of the flow of fluids on a centrifugal platform. Valving systems are also critical in isolating chambers from the rest of a fluidic network at required times, in effectively directing the reagents to the target location, in serial dilutions, and in integration of multiple other processes on a single CD. In this paper, we review the various available fluidic valving systems, discuss their working principles, and evaluate their compatibility with CD fluidic platforms. We categorize the presented valving systems into either \u201cactive\u201d, \u201cpassive\u201d, or \u201chybrid\u201d\u2014based on their actuation mechanism that can be mechanical, thermal, hydrophobic\/hydrophilic, solubility-based, phase-change, and others. Important topics such as their actuation mechanism, governing physics, variability of performance, necessary disc spin rate for valve actuation, valve response time, and other parameters are discussed. The applicability of some types of valves for specialized functions such as reagent storage, flow control, and other applications is summarized.<\/jats:p>","DOI":"10.3390\/s22228955","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T04:39:59Z","timestamp":1669005599000},"page":"8955","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Microvalves for Applications in Centrifugal Microfluidics"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8137-9996","authenticated-orcid":false,"given":"Snehan","family":"Peshin","sequence":"first","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4847-3117","authenticated-orcid":false,"given":"Marc","family":"Madou","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA"},{"name":"School of Engineering and Science, Tecnol\u00f3gico de Monterrey, Monterrey 64849, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1176-3578","authenticated-orcid":false,"given":"Lawrence","family":"Kulinsky","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1093\/clinchem\/18.8.753","article-title":"Development of a Miniature Fast Analyzer","volume":"18","author":"Burtis","year":"1972","journal-title":"Clin. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1117\/12.307314","article-title":"LabCD: A centrifuge-based microfluidic platform for diagnostics","volume":"Volume 3259","author":"Madou","year":"1998","journal-title":"Proceedings of the Systems and Technologies for Clinical Diagnostics and Drug Discovery"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1021\/ac990682c","article-title":"Microfabricated Centrifugal Microfluidic Systems:\u2009 Characterization and Multiple Enzymatic Assays","volume":"71","author":"Duffy","year":"1999","journal-title":"Anal. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0003-2670(00)00776-5","article-title":"Disc-based immunoassay microarrays","volume":"411","author":"Kido","year":"2000","journal-title":"Anal. Chim. Acta"},{"key":"ref_5","unstructured":"Gowda, H.N. (2021). Innovations in Centrifugal Microfluidics for Pathogen Detection in Water, UC Irvine."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1039\/D0LC00838A","article-title":"Rapid sample preparation for detection of antibiotic resistance on a microfluidic disc platform","volume":"21","author":"Perebikovsky","year":"2021","journal-title":"Lab. A Chip."},{"key":"ref_7","unstructured":"Madou, M., Perebikovsky, A., Liu, Y., Kido, H., Churchman, S.A., and Halford, C.W. (2021). Portable Microfludic System for Biological and Analytical Testing. (17\/054,306), U.S. Patent."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s10404-007-0196-x","article-title":"Analysis and experiment of capillary valves for microfluidics on a rotating disk","volume":"4","author":"Chen","year":"2008","journal-title":"Microfluid. Nanofluid"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S0924-4247(03)00363-7","article-title":"Passive valves based on hydrophobic microfluidics","volume":"108","author":"Feng","year":"2003","journal-title":"Sens. Actuators A Phys."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10404-009-0523-5","article-title":"Serial siphon valving for centrifugal microfluidic platforms","volume":"9","author":"Siegrist","year":"2010","journal-title":"Microfluid Nanofluid"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1038\/s41467-018-08091-z","article-title":"A micro-dispenser for long-term storage and controlled release of liquids","volume":"10","author":"Kazemzadeh","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1039\/C6LC00568C","article-title":"Xurography actuated valving for centrifugal flow control","volume":"16","author":"Kinahan","year":"2016","journal-title":"Lab. Chip."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1149\/MA2021-01601611mtgabs","article-title":"Reusable Capillary Flow-Based Wax Switch Valve for Centrifugal Microfluidics","volume":"MA2021-01","author":"Peshin","year":"2021","journal-title":"Meet. Abstr."},{"key":"ref_14","first-page":"554","article-title":"A phase change microvalve using a meltable magnetic material: Ferro-wax","volume":"1","author":"Oh","year":"2005","journal-title":"Micro Total Anal. Syst."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.1039\/C6LC00629A","article-title":"A lab-on-a-disc with reversible and thermally stable diaphragm valves","volume":"16","author":"Kim","year":"2016","journal-title":"Lab. Chip."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1039\/b802853b","article-title":"Electrically controlled microvalves to integrate microchip polymerase chain reaction and capillary electrophoresis","volume":"8","author":"Kaigala","year":"2008","journal-title":"Lab. Chip."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1039\/b616112j","article-title":"Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices","volume":"7","author":"Park","year":"2007","journal-title":"Lab. Chip."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"R13","DOI":"10.1088\/0960-1317\/16\/5\/R01","article-title":"A Review of Microvalves","volume":"16","author":"Oh","year":"2006","journal-title":"J. Micromech. Microeng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3390\/mi2020179","article-title":"Microvalves and Micropumps for BioMEMS","volume":"2","author":"Au","year":"2011","journal-title":"Micromachines"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Qian, J.-Y., Hou, C.-W., Li, X.-J., and Jin, Z.-J. (2020). Actuation Mechanism of Microvalves: A Review. Micromachines, 11.","DOI":"10.3390\/mi11020172"},{"key":"ref_21","first-page":"1","article-title":"Systematic Review of Centrifugal Valving Based on Digital Twin Modeling towards Highly Integrated Lab-on-a-Disc Systems","volume":"7","year":"2021","journal-title":"Microsyst. Nanoeng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2888","DOI":"10.1039\/c3lc50404b","article-title":"Miniature stick-packaging\u2013an industrial technology for pre-storage and release of reagents in lab-on-a-chip systems","volume":"13","author":"Barb","year":"2013","journal-title":"Lab. Chip."},{"key":"ref_23","unstructured":"Baumann, D., Hin, S., Stumpf, F., Klein, V., Mitsakakis, K., Kosse, D., von Stetten, F., Zengerle, R., and Mark, D. (2015, January 25\u201329). Fully Automated Stick-Packaging for Precise Liquid Reagent Pre-Storage and Release in Lab-on-a-Chip Disposables. Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Gyeongju, Republic of Korea."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Stumpf, F., Schwemmer, F., Hutzenlaub, T., Baumann, D., Strohmeier, O., von Stetten, F., Zengerle, R., and Mark, D. (2015, January 21\u201325). Automated sample-to-answer nucleic acid testing with frequency controlled reagent release from cartridge integrated stickpacks. Proceedings of the 2015 Transducers\u20142015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), Anchorage, AK, USA.","DOI":"10.1109\/TRANSDUCERS.2015.7181030"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1039\/B406699E","article-title":"Frequency-dependent transversal flow control in centrifugal microfluidics","volume":"5","author":"Brenner","year":"2005","journal-title":"Lab. Chip."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1039\/b820557b","article-title":"Microfluidic lab-on-a-chip platforms: Requirements, characteristics and applications","volume":"39","author":"Mark","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.snb.2007.07.079","article-title":"Passive flow switching valves on a centrifugal microfluidic platform","volume":"128","author":"Kim","year":"2008","journal-title":"Sens. Actuators B Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"024101","DOI":"10.1063\/1.4867241","article-title":"Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips","volume":"8","author":"Deng","year":"2014","journal-title":"Biomicrofluidics"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Al-Faqheri, W., Ibrahim, F., Thio, T.H.G., Moebius, J., Joseph, K., Arof, H., and Madou, M. (2013). Vacuum\/Compression Valving (VCV) Using Parrafin-Wax on a Centrifugal Microfluidic CD Platform. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058523"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4658","DOI":"10.3390\/s150304658","article-title":"Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform","volume":"15","author":"Ibrahim","year":"2015","journal-title":"Sensors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.snb.2015.05.051","article-title":"Water-clock-based autonomous flow sequencing in steadily rotating centrifugal microfluidic device","volume":"220","author":"Ukita","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.jcis.2006.10.077","article-title":"How the capillary burst microvalve works","volume":"306","author":"Cho","year":"2007","journal-title":"J. Colloid Interface Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s11517-012-1020-7","article-title":"Theoretical development and critical analysis of burst frequency equations for passive valves on centrifugal microfluidic platforms","volume":"51","author":"Thio","year":"2013","journal-title":"Med. Biol. Eng. Comput."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"van den Berg, A., Olthuis, W., and Bergveld, P. Design Analyses of Capillary Burst Valves in Centrifugal Microfluidics. Proceedings of the Micro Total Analysis Systems 2000.","DOI":"10.1007\/978-94-017-2264-3"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s10404-011-0878-2","article-title":"Suction-enhanced siphon valves for centrifugal microfluidic platforms","volume":"12","author":"Gorkin","year":"2012","journal-title":"Microfluid. Nanofluid."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"12694","DOI":"10.1021\/ja982975d","article-title":"Totally Synthetic Polymer Gels Responding to External Glucose Concentration:\u2009 Their Preparation and Application to On\u2013Off Regulation of Insulin Release","volume":"120","author":"Kataoka","year":"1998","journal-title":"J. Am. Chem. Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2894","DOI":"10.1039\/c2lc20973j","article-title":"Centrifugo-pneumatic valving utilizing dissolvable films","volume":"12","author":"Iii","year":"2012","journal-title":"Lab. Chip."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1117\/12.359339","article-title":"Hydrophobic microfluidics","volume":"Volume 3877","author":"McNeely","year":"1999","journal-title":"Proceedings of the Microfluidic Devices and Systems II"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1021\/ac061692y","article-title":"Parallel Nanoliter Microfluidic Analysis System","volume":"79","author":"Andersson","year":"2007","journal-title":"Anal. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1007\/s10404-010-0759-0","article-title":"Aliquoting on the centrifugal microfluidic platform based on centrifugo-pneumatic valves","volume":"10","author":"Mark","year":"2011","journal-title":"Microfluid. Nanofluid."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.colsurfb.2007.03.015","article-title":"A novel, compact disk-like centrifugal microfluidics system for cell lysis and sample homogenization","volume":"58","author":"Kido","year":"2007","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"760816","DOI":"10.1155\/S1463924695000174","article-title":"Centrifugation and Capillarity Integrated into a Multiple Analyte Whole Blood Analyser","volume":"17","author":"Schembri","year":"1995","journal-title":"J. Anal. Methods Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s10544-007-9076-0","article-title":"Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk","volume":"9","author":"Steigert","year":"2007","journal-title":"Biomed. Microdevices"},{"key":"ref_44","unstructured":"Shin, A., Shiri, R., Liu, Y., Peshin, S., George, D., and Madou, M. (2022). Burstable Liquid Storage Package for Biological Materials and Valve Substitution. (17\/721,826), U.S. Patent."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1039\/c004980h","article-title":"Optically addressable single-use microfluidic valves by laser printer lithography","volume":"10","author":"Kurzbuch","year":"2010","journal-title":"Lab. Chip."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1007\/s12010-015-1546-x","article-title":"Automated Centrifugal-Microfluidic Platform for DNA Purification Using Laser Burst Valve and Coriolis Effect","volume":"175","author":"Choi","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Clark, C.P., Woolf, M.S., Karstens, S.L., Lewis, H.M., Nauman, A.Q., and Landers, J.P. (2020). Closable Valves and Channels for Polymeric Microfluidic Devices. Micromachines, 11.","DOI":"10.3390\/mi11070627"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Peshin, S., George, D., Shiri, R., Kulinsky, L., and Madou, M. (2022). Capillary Flow-Driven and Magnetically Actuated Multi-Use Wax Valves for Controlled Sealing and Releasing of Fluids on Centrifugal Microfluidic Platforms. Micromachines, 13.","DOI":"10.3390\/mi13020303"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1126\/science.288.5463.113","article-title":"Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography","volume":"288","author":"Unger","year":"2000","journal-title":"Science"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0925-4005(02)00468-9","article-title":"Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices","volume":"89","author":"Grover","year":"2003","journal-title":"Sens. Actuators B Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1109\/JMEMS.2015.2395379","article-title":"A Novel Magnetic Active Valve for Lab-on-CD Technology","volume":"24","author":"Carpentras","year":"2015","journal-title":"J. Microelectromech. Syst."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1039\/c2lc00015f","article-title":"Capillary soft valves for microfluidics","volume":"12","author":"Hitzbleck","year":"2012","journal-title":"Lab. Chip."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1088\/0960-1317\/14\/11\/010","article-title":"Flow control for capillary-pumped microfluidic systems","volume":"14","author":"Vestad","year":"2004","journal-title":"J. Micromech. Microeng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1039\/C4LC00947A","article-title":"From cellular lysis to microarray detection, an integrated thermoplastic elastomer (TPE) point of care Lab on a Disc","volume":"15","author":"Roy","year":"2014","journal-title":"Lab. Chip."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/35007047","article-title":"Functional hydrogel structures for autonomous flow control inside microfluidic channels","volume":"404","author":"Beebe","year":"2000","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1021\/ac026455j","article-title":"Flow Control Valves for Analytical Microfluidic Chips without Mechanical Parts Based on Thermally Responsive Monolithic Polymers","volume":"75","author":"Yu","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/346345a0","article-title":"Phase transition in polymer gels induced by visible light","volume":"346","author":"Suzuki","year":"1990","journal-title":"Nature"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1126\/science.269.5223.525","article-title":"Synthesis and Application of Modulated Polymer Gels","volume":"269","author":"Hu","year":"1995","journal-title":"Science"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/s41378-021-00260-3","article-title":"Thermally-actuated microfluidic membrane valve for point-of-care applications","volume":"7","author":"Sesen","year":"2021","journal-title":"Microsyst. Nanoeng."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1088\/0960-1317\/14\/2\/011","article-title":"Ice valve for a mini\/micro flow channel","volume":"14","author":"Gui","year":"2003","journal-title":"J. Micromech. Microeng."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/j.snb.2011.11.080","article-title":"Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving","volume":"161","author":"Amasia","year":"2012","journal-title":"Sens. Actuators B Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1021\/ac0353029","article-title":"Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection","volume":"76","author":"Liu","year":"2004","journal-title":"Anal. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.1021\/ac0352934","article-title":"Phase Change Microvalve for Integrated Devices","volume":"76","author":"Pal","year":"2004","journal-title":"Anal. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.sna.2014.12.018","article-title":"Development of novel passive check valves for the microfluidic CD platform","volume":"222","author":"Ibrahim","year":"2015","journal-title":"Sens. Actuators A Phys."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1039\/c3lc41275j","article-title":"Rapid patterning of \u2018tunable\u2019 hydrophobic valves on disposable microchips by laser printer lithography","volume":"13","author":"Ouyang","year":"2013","journal-title":"Lab. Chip."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1039\/b820321k","article-title":"A fully automated immunoassay from whole blood on a disc","volume":"9","author":"Lee","year":"2009","journal-title":"Lab. Chip."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1023\/A:1015887213431","article-title":"Hydrogels: Swelling, Drug Loading, and Release","volume":"9","author":"Kim","year":"1992","journal-title":"Pharm. Res."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Jayaramudu, T., Ko, H.-U., Kim, H.C., Kim, J.W., and Kim, J. (2019). Swelling Behavior of Polyacrylamide\u2013Cellulose Nanocrystal Hydrogels: Swelling Kinetics, Temperature, and pH Effects. Materials, 12.","DOI":"10.3390\/ma12132080"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0168-3659(91)90057-K","article-title":"Controlling the swelling characteristics of temperature-sensitive cellulose ether hydrogels","volume":"17","author":"Harsh","year":"1991","journal-title":"J. Control. Release"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"5528","DOI":"10.1021\/ma00046a063","article-title":"Mutual influence of pH and temperature on the swelling of ionizable and thermosensitive hydrogels","volume":"25","author":"Feil","year":"1992","journal-title":"Macromolecules"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1039\/B913219H","article-title":"Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples","volume":"10","author":"Siegrist","year":"2010","journal-title":"Lab. Chip."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.1063\/1.1367010","article-title":"Responsive biomimetic hydrogel valve for microfluidics","volume":"78","author":"Yu","year":"2001","journal-title":"Appl. Phys. Lett."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1108\/13552540010309868","article-title":"Characterization of the laminated object manufacturing (LOM) process","volume":"6","author":"Park","year":"2000","journal-title":"Rapid Prototyp. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1007\/s10404-014-1492-x","article-title":"Multifunctional wax valves for liquid handling and incubation on a microfluidic CD","volume":"18","author":"Kong","year":"2015","journal-title":"Microfluid. Nanofluid."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/22\/8955\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:36Z","timestamp":1760145696000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/22\/8955"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,18]]},"references-count":74,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["s22228955"],"URL":"https:\/\/doi.org\/10.3390\/s22228955","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,18]]}}}