{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:37:14Z","timestamp":1760233034984,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Institute of Marine Science and Technology","award":["20170263","S3073225","S2977279","2020R1F1A1051994"],"award-info":[{"award-number":["20170263","S3073225","S2977279","2020R1F1A1051994"]}]},{"DOI":"10.13039\/501100013129","name":"Technology Development Program of the Korean Ministry of SMEs and Startups (MSS)","doi-asserted-by":"publisher","award":["20170263","S3073225","S2977279","2020R1F1A1051994"],"award-info":[{"award-number":["20170263","S3073225","S2977279","2020R1F1A1051994"]}],"id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Research Foundation of Korea (NRF) funded by the Korean Ministry of Science and ICT (MSIT)","award":["20170263","S3073225","S2977279","2020R1F1A1051994"],"award-info":[{"award-number":["20170263","S3073225","S2977279","2020R1F1A1051994"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we propose a single camera-based dual-channel near-infrared (NIR) fluorescence imaging system that produces color and dual-channel NIR fluorescence images in real time. To simultaneously acquire color and dual-channel NIR fluorescence images of two fluorescent agents, three cameras and additional optical parts are generally used. As a result, the volume of the image acquisition unit increases, interfering with movements during surgical procedures and increasing production costs. In the system herein proposed, instead of using three cameras, we set a single camera equipped with two image sensors that can simultaneously acquire color and single-channel NIR fluorescence images, thus reducing the volume of the image acquisition unit. The single-channel NIR fluorescence images were time-divided into two channels by synchronizing the camera and two excitation lasers, and the noise caused by the crosstalk effect between the two fluorescent agents was removed through image processing. To evaluate the performance of the system, experiments were conducted for the two fluorescent agents to measure the sensitivity, crosstalk effect, and signal-to-background ratio. The compactness of the resulting image acquisition unit alleviates the inconvenient movement obstruction of previous devices during clinical and animal surgery and reduces the complexity and costs of the manufacturing process, which may facilitate the dissemination of this type of system.<\/jats:p>","DOI":"10.3390\/s22249758","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T03:32:32Z","timestamp":1670902352000},"page":"9758","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system"],"prefix":"10.3390","volume":"22","author":[{"given":"Janghoon","family":"Choi","sequence":"first","affiliation":[{"name":"Intelligent Photonic IoT Research Center, Korea Photonics Technology Institute, Gwangju 61007, Republic of Korea"},{"name":"Department of Biomedical Science & Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Geun","family":"Shin","sequence":"additional","affiliation":[{"name":"Optical Precision Measurement Research Center, Korea Photonics Technology Institute, Gwangju 61007, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon-Oh","family":"Tak","sequence":"additional","affiliation":[{"name":"Intelligent Photonic IoT Research Center, Korea Photonics Technology Institute, Gwangju 61007, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7939-5428","authenticated-orcid":false,"given":"Youngseok","family":"Seo","sequence":"additional","affiliation":[{"name":"WONTECH Co., Ltd., Daejeon 34028, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4499-0803","authenticated-orcid":false,"given":"Jonghyun","family":"Eom","sequence":"additional","affiliation":[{"name":"Intelligent Photonic IoT Research Center, Korea Photonics Technology Institute, Gwangju 61007, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21708","article-title":"Cancer statistics, 2022","volume":"72","author":"Siegel","year":"2022","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1164\/ajrccm.165.4.2107006","article-title":"Screening for Lung Cancer with Low-Dose Spiral Computed Tomography","volume":"165","author":"Swensen","year":"2002","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1200\/JCO.2007.14.3065","article-title":"New Technologies for Human Cancer Imaging","volume":"26","author":"Frangioni","year":"2008","journal-title":"J. Clin. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.3390\/cancers6041821","article-title":"Positron Emission Tomography (PET) in Oncology","volume":"6","author":"Gallamini","year":"2014","journal-title":"Cancers"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.cbpa.2003.08.007","article-title":"In vivo near-infrared fluorescence imaging","volume":"7","author":"Frangioni","year":"2003","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.2325\/jbcs.12.211","article-title":"Fluorescence navigation with indocyanine green for detecting sentinel lymph nodes in breast cancer","volume":"12","author":"Kitai","year":"2005","journal-title":"Breast Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.amjsurg.2007.02.032","article-title":"Intraoperative identification of sentinel lymph nodes by near-infrared fluorescence imaging in patients with breast cancer","volume":"195","author":"Tagaya","year":"2008","journal-title":"Am. J. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s10549-013-2802-9","article-title":"Near-infrared fluorescence sentinel lymph node mapping in breast cancer: A multicenter experience","volume":"143","author":"Verbeek","year":"2013","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"89.e1","DOI":"10.1016\/j.ajog.2011.07.039","article-title":"Optimization of near-infrared fluorescent sentinel lymph node mapping for vulvar cancer","volume":"206","author":"Hutteman","year":"2012","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1002\/jso.23740","article-title":"Optimization of sentinel lymph node mapping in bladder cancer using near-infrared fluorescence imaging","volume":"110","author":"Schaafsma","year":"2014","journal-title":"J. Surg. Oncol."},{"key":"ref_11","first-page":"93","article-title":"Dose optimization for near-infrared fluorescence sentinel lymph node mapping in patients with melanoma","volume":"168","author":"Schaafsma","year":"2012","journal-title":"Br. J. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Demarchi, M., Seeliger, B., Lifante, J.-C., Alesina, P., and Triponez, F. (2021). Fluorescence Image-Guided Surgery for Thyroid Cancer: Utility for Preventing Hypoparathyroidism. Cancers, 13.","DOI":"10.3390\/cancers13153792"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1177\/1553350615613450","article-title":"Utility of Indocyanine Green Fluorescence Imaging for Intraoperative Localization in Reoperative Parathyroid Surgery","volume":"26","author":"Sound","year":"2015","journal-title":"Surg. Innov."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1016\/j.juro.2013.04.072","article-title":"Optimization of Near Infrared Fluorescence Tumor Localization during Robotic Partial Nephrectomy","volume":"190","author":"Angell","year":"2013","journal-title":"J. Urol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s11060-018-2854-0","article-title":"First-in-human intraoperative near-infrared fluorescence imaging of glioblastoma using cetuximab-IRDye800","volume":"139","author":"Miller","year":"2018","journal-title":"J. Neuro-Oncol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1007\/s00464-013-3305-9","article-title":"Optimization of near-infrared fluorescence cholangiography for open and laparoscopic surgery","volume":"28","author":"Verbeek","year":"2013","journal-title":"Surg. Endosc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1055\/s-0029-1244805","article-title":"Intraoperative Near-infrared Fluorescence Imaging in Perforator Flap Reconstruction: Current Research and Early Clinical Experience","volume":"26","author":"Lee","year":"2009","journal-title":"J. Reconstr. Microsurg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.ijscr.2018.11.033","article-title":"Indocyanine green fluorescence image-guided total laparoscopic living donor right hepatectomy: The first case report from Mainland China","volume":"53","author":"Meng","year":"2018","journal-title":"Int. J. Surg. Case Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3892\/ol.2020.12375","article-title":"Comparisons of ICG-fluorescence with conventional tracers in sentinel lymph node biopsy for patients with early-stage breast cancer: A meta-analysis","volume":"21","author":"Yin","year":"2020","journal-title":"Oncol. Lett."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.ejso.2014.02.225","article-title":"Real-time intraoperative detection of breast cancer using near-infrared fluorescence imaging and Methylene Blue","volume":"40","author":"Tummers","year":"2014","journal-title":"Eur. J. Surg. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Budner, O., Cwalinski, T., Skokowski, J., Marano, L., Resca, L., Cwalina, N., Kalinowski, L., Hoveling, R., Roviello, F., and Polom, K. (2022). Methylene Blue Near-Infrared Fluorescence Imaging in Breast Cancer Sentinel Node Biopsy. Cancers, 14.","DOI":"10.3390\/cancers14071817"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.surg.2015.03.027","article-title":"Intraoperative guidance in parathyroid surgery using near-infrared fluorescence imaging and low-dose Methylene Blue","volume":"158","author":"Tummers","year":"2015","journal-title":"Surgery"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.ijscr.2014.12.002","article-title":"Intraoperative near-infrared fluorescence imaging of a paraganglioma using methylene blue: A case report","volume":"6","author":"Tummers","year":"2015","journal-title":"Int. J. Surg. Case Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.surg.2009.12.003","article-title":"Real-time, near-infrared, fluorescence-guided identification of the ureters using methylene blue","volume":"148","author":"Matsui","year":"2010","journal-title":"Surgery"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.juro.2013.02.3187","article-title":"Intraoperative Near Infrared Fluorescence Guided Identification of the Ureters Using Low Dose Methylene Blue: A First in Human Experience","volume":"190","author":"Verbeek","year":"2013","journal-title":"J. Urol."},{"key":"ref_26","first-page":"279","article-title":"Colorectal cancer and adjacent normal mucosa differ in apoptotic and inflammatory protein expression","volume":"2","author":"Sun","year":"2021","journal-title":"Eng. Regen."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"17469","DOI":"10.1021\/ja4085308","article-title":"Fluorescence Discrimination of Cancer from Inflammation by Molecular Response to COX-2 Enzymes","volume":"135","author":"Zhang","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jtcvs.2008.09.082","article-title":"Real-time assessment of cardiac perfusion, coronary angiography, and acute intravascular thrombi using dual-channel near-infrared fluorescence imaging","volume":"138","author":"Tanaka","year":"2009","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1007\/s00464-010-1405-3","article-title":"Laparoscopic detection of sentinel node in gastric cancer surgery by indocyanine green fluorescence imaging","volume":"25","author":"Miyashiro","year":"2010","journal-title":"Surg. Endosc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1245\/s10434-009-0594-2","article-title":"The FLARE\u2122 Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping","volume":"16","author":"Troyan","year":"2009","journal-title":"Ann. Surg. Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Choi, J., Shin, J.G., Kwon, H.-S., Tak, Y.-O., Park, H.J., Ahn, J.-C., Eom, J.B., Seo, Y., Park, J.W., and Choi, Y. (2022). Development of Intraoperative Near-Infrared Fluorescence Imaging System Using a Dual-CMOS Single Camera. Sensors, 22.","DOI":"10.3390\/s22155597"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hovi, A., Forsstr\u00f6m, P., M\u00f5ttus, M., and Rautiainen, M. (2017). Evaluation of Accuracy and Practical Applicability of Methods for Measuring Leaf Reflectance and Transmittance Spectra. Remote Sens., 10.","DOI":"10.3390\/rs10010025"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Marshall, J.L., Williams, P., Rheault, J.-P., Prochaska, T., Allen, R.D., and DePoy, D.L. (2014, January 8). Characterization of the Reflectivity of Various Black Materials. Proceedings of the Ground-Based and Airborne Instrumentation for Astronomy V, Montr\u00e9al, QC, Canada.","DOI":"10.1117\/12.2056729"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.2147\/IJN.S295234","article-title":"Interventional NIR Fluorescence Imaging of Cancer: Review on Next Generation of Dye-Loaded Protein-Based Nanoparticles for Real-Time Feedback During Cancer Surgery","volume":"16","author":"Borlan","year":"2021","journal-title":"Int. J. Nanomed."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/24\/9758\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:40:12Z","timestamp":1760146812000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/22\/24\/9758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,13]]},"references-count":34,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["s22249758"],"URL":"https:\/\/doi.org\/10.3390\/s22249758","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2022,12,13]]}}}