{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T04:56:49Z","timestamp":1779080209277,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004794","name":"CNRS","doi-asserted-by":"publisher","award":["MITI 2022"],"award-info":[{"award-number":["MITI 2022"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"CNRS","doi-asserted-by":"publisher","award":["BIOEPC 2022"],"award-info":[{"award-number":["BIOEPC 2022"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"CNRS","doi-asserted-by":"publisher","award":["PHC POLONIUM 2021"],"award-info":[{"award-number":["PHC POLONIUM 2021"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institut Carnot LSI","award":["MITI 2022"],"award-info":[{"award-number":["MITI 2022"]}]},{"name":"Institut Carnot LSI","award":["BIOEPC 2022"],"award-info":[{"award-number":["BIOEPC 2022"]}]},{"name":"Institut Carnot LSI","award":["PHC POLONIUM 2021"],"award-info":[{"award-number":["PHC POLONIUM 2021"]}]},{"name":"Campus France","award":["MITI 2022"],"award-info":[{"award-number":["MITI 2022"]}]},{"name":"Campus France","award":["BIOEPC 2022"],"award-info":[{"award-number":["BIOEPC 2022"]}]},{"name":"Campus France","award":["PHC POLONIUM 2021"],"award-info":[{"award-number":["PHC POLONIUM 2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx), and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time, according to different storage conditions (in PBS solution at temperatures of 4 \u00b0C and 37 \u00b0C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable of maintaining its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the lifetime of the biosensor.<\/jats:p>","DOI":"10.3390\/s23010465","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T03:08:59Z","timestamp":1672628939000},"page":"465","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2831-2626","authenticated-orcid":false,"given":"Ayman","family":"Chmayssem","sequence":"first","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS, UMR 5525, VetAgro Sup, Grenoble INP, INSERM, TIMC, 38000 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9368-3565","authenticated-orcid":false,"given":"Ibrahim","family":"Shalayel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS, UMR 5525, VetAgro Sup, Grenoble INP, INSERM, TIMC, 38000 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Marinesco","sequence":"additional","affiliation":[{"name":"Plate-Forme Technologique BELIV, Lyon Neuroscience Research Center, CNRS UMR5292, INSERM U1028, Universit\u00e9 Claude Bernard Lyon 1, 69373 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelkader","family":"Zebda","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS, UMR 5525, VetAgro Sup, Grenoble INP, INSERM, TIMC, 38000 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1039\/c2ra22351a","article-title":"Recent Advances in Electrochemical Glucose Biosensors: A Review","volume":"3","author":"Chen","year":"2013","journal-title":"RSC Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1021\/cr068123a","article-title":"Electrochemical Glucose Biosensors","volume":"108","author":"Wang","year":"2008","journal-title":"Chem. 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