{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:46:36Z","timestamp":1760147196623,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of National Economy of Hungary","award":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"],"award-info":[{"award-number":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"]}]},{"name":"Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund (NKFIA)","award":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"],"award-info":[{"award-number":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"]}]},{"name":"Cooperative Doctoral Programme","award":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"],"award-info":[{"award-number":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"]}]},{"name":"Hungarian Academy of Sciences via Lend\u00fclet (Momentum) Programme","award":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"],"award-info":[{"award-number":["VEKOP-2.2.1-16-2017-00001","INBIOM TKP2021-EGA-04","KKP 129936","KDP-2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (\u00d8 = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 \u00b5m) at different flow rates (0.5, 1, 2 \u00b5L\/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli (E. coli), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae, and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.<\/jats:p>","DOI":"10.3390\/s23020800","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T04:59:58Z","timestamp":1673413198000},"page":"800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels"],"prefix":"10.3390","volume":"23","author":[{"given":"Anita","family":"B\u00e1nyai","sequence":"first","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"},{"name":"77 Elektronika Ltd., Feh\u00e9rv\u00e1ri Str. 98, H-1111 Budapest, Hungary"},{"name":"Doctoral School on Materials Sciences and Technologies, \u00d3buda University, B\u00e9csi Str. 96\/B, H-1034 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enik\u0151","family":"Farkas","sequence":"additional","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajnalka","family":"Jankovics","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem Str. 10, H-8200 Veszpr\u00e9m, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1900-9422","authenticated-orcid":false,"given":"Inna","family":"Sz\u00e9k\u00e1cs","sequence":"additional","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4013-499X","authenticated-orcid":false,"given":"Eszter Leel\u0151ssyn\u00e9","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6498-8172","authenticated-orcid":false,"given":"Ferenc","family":"Vonderviszt","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem Str. 10, H-8200 Veszpr\u00e9m, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8617-2302","authenticated-orcid":false,"given":"R\u00f3bert","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1t\u00e9","family":"Varga","sequence":"additional","affiliation":[{"name":"77 Elektronika Ltd., Feh\u00e9rv\u00e1ri Str. 98, H-1111 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8022-4367","authenticated-orcid":false,"given":"P\u00e9ter","family":"F\u00fcrjes","sequence":"additional","affiliation":[{"name":"Centre for Energy Research, Institute of Technical Physics and Materials Science, E\u00f6tv\u00f6s Lor\u00e1nd Research Network, Konkoly Thege Mikl\u00f3s Str. 29-33, H-1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/189209a0","article-title":"Radial Particle Displacements in Poiseuille Flow of Suspensions","volume":"189","author":"Silberberg","year":"1961","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"44072","DOI":"10.1038\/srep44072","article-title":"Dean Flow Dynamics in Low-Aspect Ratio Spiral Microchannels","volume":"7","author":"Nivedita","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"034117","DOI":"10.1063\/1.5109004","article-title":"New insights into the physics of inertial microfluidics in curved microchannels. I. Relaxing the fixed inflection point assumption","volume":"13","author":"Rafeie","year":"2019","journal-title":"Biomicrofluidics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1039\/C8LC00973B","article-title":"Single stream inertial focusing in low aspect-ratio triangular microchannels","volume":"19","author":"Mukherjee","year":"2018","journal-title":"Lab A Chip"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1039\/C5LC01100K","article-title":"Inertial focusing in non-rectangular cross-section microchannels and manipulation of accessible focusing positions","volume":"16","author":"Kim","year":"2016","journal-title":"Lab A Chip"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"18892","DOI":"10.1073\/pnas.0704958104","article-title":"Continuous inertial focusing, ordering, and separation of particles in microchannels","volume":"104","author":"Irimia","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41378-018-0005-6","article-title":"Sheathless inertial cell focusing and sorting with serial reverse wavy channel structures","volume":"4","author":"Zhou","year":"2018","journal-title":"Microsyst. Nanoeng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s10404-018-2082-0","article-title":"Inertial focusing of microparticles in curvilinear microchannels with different curvature angles","volume":"22","author":"Karimzadehkhouei","year":"2018","journal-title":"Microfluid. Nanofluid."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.1038\/srep03340","article-title":"Particle Focusing in Curved Microfluidic Channels","volume":"3","author":"Martel","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"084001","DOI":"10.1088\/1361-6439\/aa6b14","article-title":"High pressure inertial focusing for separating and concentrating bacteria at high throughput","volume":"27","author":"Cruz","year":"2017","journal-title":"J. Micromech. Microeng."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"24105","DOI":"10.1063\/1.4870399","article-title":"High-throughput particle separation and concentration using spiral inertial filtration","volume":"8","author":"Burke","year":"2014","journal-title":"Biomicrofluidics"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1039\/b908271a","article-title":"Inertial microfluidics for continuous particle separation in spiral microchannels","volume":"9","author":"Kuntaegowdanahalli","year":"2009","journal-title":"Lab Chip"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Enders, A., Preuss, J.-A., and Bahnemann, J. (2021). 3D Printed Microfluidic Spiral Separation Device for Continuous, Pulsation-Free and Controllable CHO Cell Retention. Micromachines, 12.","DOI":"10.3390\/mi12091060"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3862","DOI":"10.1021\/ac100387b","article-title":"Particle Focusing in Staged Inertial Microfluidic Devices for Flow Cytometry","volume":"82","author":"Oakey","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1089\/jamp.2020.1616","article-title":"Breathing Is Enough: For the Spread of Influenza Virus and SARS-CoV-2 by Breathing Only","volume":"33","author":"Scheuch","year":"2020","journal-title":"J. Aerosol Med. Pulm. Drug Deliv."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.24171\/j.phrp.2020.11.1.02","article-title":"Identification of Coronavirus Isolated from a Patient in Korea with COVID-19","volume":"11","author":"Kim","year":"2020","journal-title":"Osong Public Health Res. Perspect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1021\/acsnano.9b06123","article-title":"Oscillatory Viscoelastic Microfluidics for Efficient Focusing and Separation of Nanoscale Species","volume":"14","author":"Asghari","year":"2019","journal-title":"ACS Nano"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1700153","DOI":"10.1002\/advs.201700153","article-title":"High-Throughput Inertial Focusing of Micrometer- and Sub-Micrometer-Sized Particles Separation","volume":"4","author":"Wang","year":"2017","journal-title":"Adv. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.1039\/c1lc20514e","article-title":"Rapid prototyping polymers for microfluidic devices and high pressure injections","volume":"11","author":"Sollier","year":"2011","journal-title":"Lab A Chip"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"B\u00e1nyai, A., T\u00f3th, E.L., Varga, M., and F\u00fcrjes, P. (2022). Geometry-Dependent Efficiency of Dean-Flow Affected Lateral Particle Focusing and Separation in Periodically Inhomogeneous Microfluidic Channels. Sensors, 22.","DOI":"10.3390\/s22093474"},{"key":"ref_21","unstructured":"COMSOL (2022, December 23). COMSOL: Multiphysics Software for Optimizing Designs. Available online: https:\/\/www.comsol.com\/."},{"key":"ref_22","unstructured":"Nguyen, N.-T. (2011). Micromixers: Fundamentals, Design and Fabrication, Elsevier."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s10404-018-2042-8","article-title":"A numerical model-assisted experimental design study of inertia-based particle focusing in stepped microchannels","volume":"22","author":"Winzen","year":"2018","journal-title":"Microfluid. Nanofluid."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"032007","DOI":"10.1063\/1.5018714","article-title":"Inertial migrations of cylindrical particles in rectangular microchannels: Variations of equilibrium positions and equivalent diameters","volume":"30","author":"Su","year":"2018","journal-title":"Phys. Fluids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1126\/science.1182105","article-title":"Conformational Spread as a Mechanism for Cooperativity in the Bacterial Flagellar Switch","volume":"327","author":"Bai","year":"2010","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2013925118","DOI":"10.1073\/pnas.2013925118","article-title":"Patterns of bacterial motility in microfluidics-confining environments","volume":"118","author":"Perumal","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.cis.2014.03.002","article-title":"Hydrodynamic lift of vesicles and red blood cells in flow\u2014From F\u00e5hr\u00e6us & Lindqvist to microfluidic cell sorting","volume":"208","author":"Geislinger","year":"2014","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"042710","DOI":"10.1103\/PhysRevE.92.042710","article-title":"Dynamics of a single red blood cell in simple shear flow","volume":"92","author":"Sinha","year":"2015","journal-title":"Phys. Rev. E"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"13739","DOI":"10.1038\/s41598-018-31726-6","article-title":"High-Reynolds Microfluidic Sorting of Large Yeast Populations","volume":"8","author":"Keinan","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1093\/protein\/gzs003","article-title":"A polymerizable GFP variant","volume":"25","author":"Klein","year":"2012","journal-title":"Protein Eng. Des. Sel."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1177\/09544089211051630","article-title":"Numerical analysis of non-aligned inputs M-type micromixers with different shaped obstacles for biomedical applications","volume":"236","author":"Irfan","year":"2021","journal-title":"Proc. Inst. Mech. Eng. Part E: J. Process. Mech. Eng."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1574-6941.2001.tb00783.x","article-title":"Feasibility of using GFP-expressing Escherichia coli, coupled with fluorimetry, to determine protozoan ingestion rates","volume":"35","author":"Parry","year":"2001","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"872","DOI":"10.3934\/microbiol.2017.4.872","article-title":"Rapid loss of a green fluorescent plasmid in Escherichia coli O157:H7","volume":"3","author":"Persad","year":"2017","journal-title":"AIMS Microbiol."},{"key":"ref_34","unstructured":"Britannica (2022, November 16). Bacteria\u2014Capsules and Slime Layers. Available online: https:\/\/www.britannica.com\/science\/bacteria\/Diversity-of-structure-of-bacteria."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1364\/BOE.2.001803","article-title":"Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level","volume":"2","author":"Kinnunen","year":"2011","journal-title":"Biomed. Opt. Express"},{"key":"ref_36","unstructured":"Laboratory Continuing Education (2022, November 16). Red Blood Cell (RBC) Size Variation. Available online: https:\/\/www.labce.com\/spg579126_red_blood_cell_rbc_size_variation.aspx."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Feldmann, H. (2011). Yeast: Molecular and Cell Biology, John Wiley & Sons.","DOI":"10.1002\/9783527659180"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.mne.2019.01.002","article-title":"Inertial focusing of cancer cell lines in curvilinear microchannels","volume":"2","author":"Ozbey","year":"2019","journal-title":"Micro Nano Eng."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/2\/800\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:05:48Z","timestamp":1760119548000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/2\/800"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,10]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["s23020800"],"URL":"https:\/\/doi.org\/10.3390\/s23020800","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2023,1,10]]}}}