{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T09:52:12Z","timestamp":1773481932060,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EEC-1648035"],"award-info":[{"award-number":["EEC-1648035"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Magnetoelastic sensors, which undergo mechanical resonance when interrogated with magnetic fields, can be functionalized to measure various physical quantities and chemical\/biological analytes by tracking their resonance behaviors. The unique wireless and functionalizable nature of these sensors makes them good candidates for biological sensing applications, from the detection of specific bacteria to tracking force loading inside the human body. In this study, we evaluate the viability of magnetoelastic sensors based on a commercially available magnetoelastic material (Metglas 2826 MB) for wirelessly monitoring the attachment and growth of human mesenchymal stromal cells (hMSCs) in 2D in vitro cell culture. The results indicate that the changes in sensor resonance are linearly correlated with cell quantity. Experiments using a custom-built monitoring system also demonstrated the ability of this technology to collect temporal profiles of cell growth, which could elucidate key stages of cell proliferation based on acute features in the profile. Additionally, there was no observed change in the morphology of cells after they were subjected to magnetic and mechanical stimuli from the monitoring system, indicating that this method for tracking cell growth may have minimal impact on cell quality and potency.<\/jats:p>","DOI":"10.3390\/s23041832","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T01:56:48Z","timestamp":1675735008000},"page":"1832","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Magnetoelastic Monitoring System for Tracking Growth of Human Mesenchymal Stromal Cells"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0096-243X","authenticated-orcid":false,"given":"William S.","family":"Skinner","sequence":"first","affiliation":[{"name":"Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0211-850X","authenticated-orcid":false,"given":"Sunny","family":"Zhang","sequence":"additional","affiliation":[{"name":"Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmine R.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Guldberg","sequence":"additional","affiliation":[{"name":"Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2058-1028","authenticated-orcid":false,"given":"Keat Ghee","family":"Ong","sequence":"additional","affiliation":[{"name":"Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.bej.2018.04.017","article-title":"Impact of allogeneic stem cell manufacturing decisions on cost of goods, process robustness and reimbursement","volume":"137","author":"Moncaubeig","year":"2018","journal-title":"Biochem. Eng. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.procbio.2016.04.017","article-title":"Revisiting MSC expansion from critical quality attributes to critical culture process parameters","volume":"59","author":"Martin","year":"2017","journal-title":"Process. Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.5966\/sctm.2012-0099","article-title":"Human Mesenchymal Stromal Cells: Identifying Assays to Predict Potency for Therapeutic Selection","volume":"2","author":"Deskins","year":"2013","journal-title":"Stem. Cells Transl. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1634\/stemcells.2005-0094","article-title":"In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability","volume":"23","author":"Shahdadfar","year":"2005","journal-title":"Stem Cells"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.jcyt.2013.02.010","article-title":"Immunological characterization of multipotent mesenchymal stromal cells\u2014The International Society for Cellular Therapy (ISCT) working proposal","volume":"15","author":"Krampera","year":"2013","journal-title":"Cytotherapy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s10616-011-9359-4","article-title":"Online- and offline- monitoring of stem cell expansion on microcarrier","volume":"63","author":"Justice","year":"2011","journal-title":"Cytotechnology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1016\/j.bios.2010.02.029","article-title":"Real-time electrical impedance detection of cellular activities of oral cancer cells","volume":"25","author":"Arias","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3390\/bios5020199","article-title":"Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time","volume":"5","author":"Kho","year":"2015","journal-title":"Biosensors"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1002\/bit.26328","article-title":"Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers","volume":"114","author":"Odeleye","year":"2017","journal-title":"Biotechnol. Bioeng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"025018","DOI":"10.1088\/0964-1726\/24\/2\/025018","article-title":"Geometrical modification of magnetoelastic sensors to enhance sensitivity","volume":"24","author":"Pacella","year":"2015","journal-title":"Smart Mater. Struct."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3390\/s20700294","article-title":"Wireless Magnetoelastic Resonance Sensors: A Critical Review","volume":"2","author":"Grimes","year":"2002","journal-title":"Sensors"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6494","DOI":"10.1021\/ac034562n","article-title":"Magnetoelastic Immunosensors: Amplified Mass Immunosorbent Assay for Detection of EscherichiacoliO157:H7","volume":"75","author":"Ruan","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.msec.2015.08.029","article-title":"Effect of surface roughness on performance of magnetoelastic biosensors for the detection of Escherichia coli","volume":"58","author":"Possan","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1109\/JSEN.2006.874450","article-title":"Quantification of multiple bioagents with wireless, remote-query magnetoelastic microsensors","volume":"6","author":"Ong","year":"2006","journal-title":"IEEE Sens. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1016\/j.bios.2005.11.007","article-title":"A rapid highly-sensitive endotoxin detection system","volume":"21","author":"Ong","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6149","DOI":"10.1007\/s00253-016-7624-3","article-title":"Antibody-based magneto-elastic biosensors: Potential devices for detection of pathogens and associated toxins","volume":"100","author":"Menti","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1016\/j.bios.2008.09.006","article-title":"Sequential detection of Salmonella typhimurium and Bacillus anthracis spores using magnetoelastic biosensors","volume":"24","author":"Huang","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1016\/j.bios.2006.06.037","article-title":"A magnetoelastic resonance biosensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium","volume":"22","author":"Guntupalli","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.mimet.2007.04.001","article-title":"Rapid and sensitive magnetoelastic biosensors for the detection of Salmonella typhimurium in a mixed microbial population","volume":"70","author":"Guntupalli","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.bios.2007.07.004","article-title":"Detection of Pseudomonas aeruginosa using a wireless magnetoelastic sensing device","volume":"23","author":"Pang","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.bios.2008.03.041","article-title":"In-situ monitoring of breast cancer cell (MCF-7) growth and quantification of the cytotoxicity of anticancer drugs fluorouracil and cisplatin","volume":"24","author":"Xiao","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3390\/bios2010057","article-title":"Fabrication of Biocompatible, Vibrational Magnetoelastic Materials for Controlling Cellular Adhesion","volume":"2","author":"Holmes","year":"2012","journal-title":"Biosensors"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"478","DOI":"10.4236\/jbise.2013.64060","article-title":"Remotely activated, vibrational magnetoelastic array system for controlling cell adhesion","volume":"06","author":"Trierweiler","year":"2013","journal-title":"J. Biomed. Sci. Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"095036","DOI":"10.1088\/0964-1726\/23\/9\/095036","article-title":"Biodegradation and biocompatibility of mechanically active magnetoelastic materials","volume":"23","author":"Holmes","year":"2014","journal-title":"Smart Mater. Struct."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Meyers, K.M., and Ong, K.G. (2021). Magnetoelastic Materials for Monitoring and Controlling Cells and Tissues. Sustainability, 13.","DOI":"10.3390\/su132413655"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1002\/bit.27680","article-title":"Magnetoelastic Sensors for Real-Time Tracking of Cell Growth","volume":"118","author":"Shekhar","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_27","unstructured":"PRNewswire (2022, May 27). Mesenchymal Stem Cells Market Size Worth $6.1 Billion By 2028: Grand View Research, Inc. Available online: https:\/\/www.prnewswire.co.uk\/news-releases\/mesenchymal-stem-cells-market-size-worth-6-1-billion-by-2028-grand-view-research-inc--873119294.html."},{"key":"ref_28","unstructured":"(2021, November 11). Metglas\u00ae 2826MB. Available online: www.metglas.com."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Skinner, W.S., Zhang, S., Guldberg, R.E., and Ong, K.G. (2022). Magnetoelastic Sensor Optimization for Improving Mass Monitoring. Sensors, 22.","DOI":"10.3390\/s22030827"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.exphem.2008.03.019","article-title":"Effects of plating density and culture time on bone marrow stromal cell characteristics","volume":"36","author":"Neuhuber","year":"2008","journal-title":"Exp. Hematol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-45352-3","article-title":"Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem\/stromal cells (hMSCs) through mechanotransduction","volume":"9","author":"Gerardo","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1083\/jcb.201210152","article-title":"Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force","volume":"201","author":"Wolf","year":"2013","journal-title":"J. Cell Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"012408","DOI":"10.1103\/PhysRevE.97.012408","article-title":"Capillary network formation from dispersed endothelial cells: Influence of cell traction, cell adhesion, and extracellular matrix rigidity","volume":"97","author":"Ramos","year":"2018","journal-title":"Phys. Rev. E"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.bpj.2016.12.012","article-title":"Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch","volume":"112","author":"Rens","year":"2017","journal-title":"Biophys. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1152\/ajpcell.67.2008","article-title":"Directing osteogenic and myogenic differentiation of MSCs: Interplay of stiffness and adhesive ligand presentation","volume":"295","author":"Rowlands","year":"2008","journal-title":"Am. Physiol. Soc."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/4\/1832\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:26:15Z","timestamp":1760120775000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/4\/1832"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,7]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["s23041832"],"URL":"https:\/\/doi.org\/10.3390\/s23041832","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,7]]}}}