{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T22:29:58Z","timestamp":1776983398774,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,3,4]],"date-time":"2023-03-04T00:00:00Z","timestamp":1677888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Hematocrit (HCT) is a crucial parameter for both adult and pediatric patients, indicating potentially severe pathological conditions. Most common methods for HCT assessment are microhematocrit and automated analyzers; however, developing countries present specific needs often not addressed by these technologies. Paper-based devices can be suitable for those environments being inexpensive, rapid, easy to use, and portable. The aim of this study is to describe and validate against a reference method, a novel HCT estimation method based on penetration velocity in lateral flow test strips complying with the requirements in low- or middle-income country (LMIC) scenarios. To calibrate and test the proposed method, 145 blood samples of 105 healthy neonates with gestational age greater than 37 weeks were collected (29 calibration set, 116 test set) in the range of HCT values (31.6\u201372.5%). The time difference (\u0394t) from the whole blood sample loading into the test strip instant till the nitrocellulose membrane saturation instant was measured by a reflectance meter. A nonlinear relation was observed between HCT and \u0394t and was estimated by a third-degree polynomial equation (R2 = 0.91) valid in 30% to 70% HCT interval. The proposed model was subsequently used to estimate HCT values on the test set showing a good agreement between the estimated HCT and the HCT measured by the reference method (r = 0.87, p &lt; 0.001), with a low mean difference of 0.53 \u00b1 5.04% and a slight trend of overestimation for higher hematocrit values. The mean absolute error was 4.29%, while the maximum absolute error was 10.69%. Although the proposed method did not present a sufficient accuracy to be used for diagnostic purposes, it could be suitable as a fast, low-cost, easy-to-use screening tool especially in LMIC scenarios.<\/jats:p>","DOI":"10.3390\/s23052813","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T02:28:34Z","timestamp":1678069714000},"page":"2813","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of a Novel Approach for Neonatal Hematocrit Screening Based on Penetration Velocity in Lateral Flow Test Strip"],"prefix":"10.3390","volume":"23","author":[{"given":"Lorenzo","family":"Zucchini","sequence":"first","affiliation":[{"name":"Department of Engineering and Architecture, University of Trieste, 34127 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8307-4360","authenticated-orcid":false,"given":"Milo\u0161","family":"Aj\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Department of Engineering and Architecture, University of Trieste, 34127 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Daniel","family":"Coda Zabetta","sequence":"additional","affiliation":[{"name":"Bilimetrix s.r.l., 34149 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Greco","sequence":"additional","affiliation":[{"name":"Bilimetrix s.r.l., 34149 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Fernetti","sequence":"additional","affiliation":[{"name":"Bilimetrix s.r.l., 34149 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Moretto","sequence":"additional","affiliation":[{"name":"Prodigys Technology s.r.l., 34149 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Pennini","sequence":"additional","affiliation":[{"name":"Prodigys Technology s.r.l., 34149 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agostino","family":"Accardo","sequence":"additional","affiliation":[{"name":"Department of Engineering and Architecture, University of Trieste, 34127 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e333","DOI":"10.1542\/peds.2008-2654","article-title":"Reference Ranges for Hematocrit and Blood Hemoglobin Concentration during the Neonatal Period: Data from a Multihospital Health Care System","volume":"123","author":"Jopling","year":"2009","journal-title":"Pediatrics"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Scholkmann, F., Ostojic, D., Isler, H., Bassler, D., Wolf, M., and Karen, T. 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