{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:08:47Z","timestamp":1773810527667,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council (NSTC) of Taiwan","award":["MOST 111-2221-E-239-008-MY2"],"award-info":[{"award-number":["MOST 111-2221-E-239-008-MY2"]}]},{"name":"National Science and Technology Council (NSTC) of Taiwan","award":["MOST 111-2221-E-239-028"],"award-info":[{"award-number":["MOST 111-2221-E-239-028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we developed a glucose fiber sensor incorporating heterodyne interferometry to measure the phase difference produced by the chemical reaction between glucose and glucose oxidase (GOx). Both theoretical and experimental results showed that the amount of phase variation is inversely proportional to glucose concentration. The proposed method provided a linear measurement range of the glucose concentration from 10 mg\/dL to 550 mg\/dL. The experimental results indicated that the sensitivity is proportional to the length of the enzymatic glucose sensor, and the optimum resolution can be obtained at a sensor length of 3 cm. The optimum resolution of the proposed method is better than 0.6 mg\/dL. Moreover, the proposed sensor demonstrates good repeatability and reliability. The average relative standard deviation (RSD) is better than 10% and satisfied the minimum requirement for point-of-care devices.<\/jats:p>","DOI":"10.3390\/s23062990","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T02:05:54Z","timestamp":1678413954000},"page":"2990","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Enzymatic Glucose Fiber Sensor for Glucose Concentration Measurement with a Heterodyne Interferometry"],"prefix":"10.3390","volume":"23","author":[{"given":"Cheng-Chih","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Electro-Optical Engineering, National United University, No. 2 Lienda, Miaoli 36063, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Yu","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Photonics Engineering, Yuan Ze University, 135, Yuan-Tung Road, Taoyuan City 32003, Taiwan"},{"name":"ASE Technology Holding Co., Ltd., Nantze Export Processing Zone, Kaohsiung 81146, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Yi","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Electro-Optical Engineering, National United University, No. 2 Lienda, Miaoli 36063, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chyan-Chyi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Electromechanical Engineering, Tamkang University, New Taipei 25137, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6494-6685","authenticated-orcid":false,"given":"Cheng-Ling","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Electro-Optical Engineering, National United University, No. 2 Lienda, Miaoli 36063, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"ref_1","unstructured":"(2022, November 03). National Diabetes Statistics Report, 2020 Edition, Available online: http:chrome-extension:\/\/efaidnbmnnnibpcajpcglclefindmkaj\/https:\/\/www.cdc.gov\/diabetes\/pdfs\/data\/statistics\/national-diabetes-statistics-report.pdf."},{"key":"ref_2","unstructured":"(2022, November 03). IDF Diabetes Atlas, 2015 Edition. Available online: http:chrome-extension:\/\/efaidnbmnnnibpcajpcglclefindmkaj\/https:\/\/www.diabetesatlas.org\/upload\/resources\/previous\/files\/7\/IDF%20Diabetes%20Atlas%207th.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.diabres.2013.11.002","article-title":"Global estimates of diabetes prevalence for 2013 and projections for 2035","volume":"103","author":"Guariguata","year":"2014","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tang, L., Chang, S.J., Chen, C.J., and Liu, J.T. (2020). Non-invasive blood glucose monitoring technology: A review. Sensors, 20.","DOI":"10.3390\/s20236925"},{"key":"ref_5","first-page":"100277","article-title":"Glucose sensing in the anterior chamber of the human eye model using supercontinuum source based dual wavelength low coherence interferometry","volume":"23","author":"John","year":"2019","journal-title":"Sens. Biosens. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1109\/JSSC.2011.2170633","article-title":"A 3-\u03bcW CMOS glucose sensor for wireless contact-lens tear glucose monitoring","volume":"47","author":"Liao","year":"2012","journal-title":"IEEE J. Solid-State Circuits"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7671","DOI":"10.1039\/D0CS00304B","article-title":"Electrochemical glucose sensors in diabetes management: An updated review (2010\u20132020)","volume":"49","author":"Teymourian","year":"2020","journal-title":"Chem. Soc. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1701150","DOI":"10.1002\/adhm.201701150","article-title":"Enzyme-based glucose sensor: From invasive to wearable device","volume":"7","author":"Lee","year":"2018","journal-title":"Adv. Healthcare Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1364\/AO.47.003828","article-title":"Determination of the refractive index and the chiral parameter of chiral solution based on chiral reflection equations and heterodyne interferometry","volume":"47","author":"Lin","year":"2008","journal-title":"Appl. Opt."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.sna.2016.04.008","article-title":"Fabrication and characterization of cascaded tapered Mach-Zehnder interferometer for refractive index sensing","volume":"244","author":"Bhardwaj","year":"2016","journal-title":"Sens. Actuator A Phys."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1364\/OSAC.415766","article-title":"Phase sensitive optical rotation measurement using the common-path heterodyne interferometry and a half-eave plate at a specific azimuth angle","volume":"4","author":"Chiu","year":"2021","journal-title":"OSA Contin."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s11082-022-04500-2","article-title":"Fiber Bragg grating refractive index sensor based on double D-shaped fiber","volume":"55","author":"Upadhyay","year":"2023","journal-title":"Opt. Quant. Electron."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhong, J., Liu, S., Zou, T., Yan, W., Chen, P., Liu, B., Sun, Z., and Wang, Y. (2022). High-sensitivity optical fiber-based glucose sensor using helical intermediate-period fiber grating. Sensors, 22.","DOI":"10.3390\/s22186824"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"163960","DOI":"10.1016\/j.ijleo.2019.163960","article-title":"S-shaped long period fiber grating glucose concentration biosensor based on immobilized glucose oxidase","volume":"203","author":"Wu","year":"2020","journal-title":"Optik"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Azkune, M., Ruiz-Rubio, L., Aldabaldetreku, G., Arrospide, E., P\u00e9rez-\u00c1lvarez, L., Bikandi, I., Zubia, J., and Vilas-Vilela, J.L. (2018). U-shaped and surface functionalized polymer optical fiber probe for glucose detection. Sensors, 18.","DOI":"10.3390\/s18010034"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5004","DOI":"10.1364\/OL.439715","article-title":"In vitro glucose concentration measurement by a reusable enzymatic glucose sensor and a highly stable circular heterodyne polarimeter","volume":"46","author":"Hsu","year":"2021","journal-title":"Opt. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"027003","DOI":"10.1117\/1.JBO.22.2.027003","article-title":"Enzyme-functionalized thin-cladding long-period fiber grating in transition mode at dispersion turning point for sugar-level and glucose detection","volume":"22","author":"Badmos","year":"2017","journal-title":"J. Biomed. Opt."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"102557","DOI":"10.1016\/j.yofte.2021.102557","article-title":"Phenylboronic acid functionalized helical long period grating for glucose sensing","volume":"64","author":"Zhou","year":"2021","journal-title":"Opt. Fiber Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"087102","DOI":"10.1117\/1.OE.60.8.087102","article-title":"Optical glucose detection using birefringent long-period fiber grating functionalized with graphene oxide","volume":"60","author":"Lee","year":"2021","journal-title":"Opt. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"131984","DOI":"10.1016\/j.snb.2022.131984","article-title":"Optical fiber SPR biosensor with a solid-phase enzymatic reaction device for glucose detection","volume":"366","author":"Zhang","year":"2022","journal-title":"Sens. Actuator B Chem."},{"key":"ref_21","first-page":"100608","article-title":"Reusable glucose fiber sensor for measuring glucose concentration in serum","volume":"10","author":"Hsu","year":"2011","journal-title":"Chin. Opt. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27560","DOI":"10.1364\/OE.18.027560","article-title":"Fabrication of glucose fiber sensor based on immobilized GOD technique for rapid measurement","volume":"18","author":"Lin","year":"2010","journal-title":"Opt. Express"},{"key":"ref_23","unstructured":"Yeh, P. (1991). Optical Waves in Layered Media, John Wiley & Sons. [1st ed.]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1177\/000456326900600108","article-title":"Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor","volume":"6","author":"Trinder","year":"1969","journal-title":"Ann. Clin. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1039\/an9729700142","article-title":"An improved colour reagent for the determination of blood glucose by the oxidase system","volume":"97","author":"Barham","year":"1972","journal-title":"Analyst"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"622","DOI":"10.2337\/diacare.10.5.622","article-title":"Evaluating clinical accuracy of systems for self-monitoring of blood glucose","volume":"10","author":"Clarke","year":"1987","journal-title":"Diabetes Care"},{"key":"ref_27","unstructured":"(2018). Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use: Guidance for Industry and Food and Drug Administration Staff."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1364\/AO.38.004089","article-title":"Heterodyne interferometer with subatomic period nonlinearity","volume":"38","author":"Wu","year":"1999","journal-title":"Appl. Opt."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3080","DOI":"10.1364\/OL.32.003080","article-title":"Slot-waveguide biochemical sensor","volume":"32","author":"Barrios","year":"2007","journal-title":"Opt. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"077101","DOI":"10.1117\/1.OE.52.7.077101","article-title":"Modeling and fabrication of evanescent waveguide-based optical sensor for sensitivity enhancement using silicon oxynitride technology","volume":"52","author":"Dutta","year":"2013","journal-title":"Opt. Eng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.aca.2004.03.052","article-title":"An optical glucose biosensor based on entrapped-glucose oxidase in silicate xerogel hybridized with hydroxyethyl carboxymethyl cellulose","volume":"514","author":"Wu","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_32","unstructured":"(1996). Certificate of Analysis of Standard Reference Material 965."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/6\/2990\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:51:29Z","timestamp":1760122289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/6\/2990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,9]]},"references-count":32,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["s23062990"],"URL":"https:\/\/doi.org\/10.3390\/s23062990","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,9]]}}}