{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T11:13:24Z","timestamp":1783250004049,"version":"3.54.6"},"reference-count":39,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation of the State Key Laboratory of NBC Protection for Civilians","award":["SKLNBC2019-05"],"award-info":[{"award-number":["SKLNBC2019-05"]}]},{"name":"Foundation of the State Key Laboratory of NBC Protection for Civilians","award":["y2021012"],"award-info":[{"award-number":["y2021012"]}]},{"name":"Detection technology and application development of nitric oxide in human exhaled air","award":["SKLNBC2019-05"],"award-info":[{"award-number":["SKLNBC2019-05"]}]},{"name":"Detection technology and application development of nitric oxide in human exhaled air","award":["y2021012"],"award-info":[{"award-number":["y2021012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, a core-shell based on the Fe3O4@SiO2@Au nanoparticle amplification technique for a surface plasmon resonance (SPR) sensor is proposed. Fe3O4@SiO2@AuNPs were used not only to amplify SPR signals, but also to rapidly separate and enrich T-2 toxin via an external magnetic field. We detected T-2 toxin using the direct competition method in order to evaluate the amplification effect of Fe3O4@SiO2@AuNPs. A T-2 toxin\u2013protein conjugate (T2-OVA) immobilized on the surface of 3-mercaptopropionic acid-modified sensing film competed with T-2 toxin to combine with the T-2 toxin antibody\u2013Fe3O4@SiO2@AuNPs conjugates (mAb-Fe3O4@SiO2@AuNPs) as signal amplification elements. With the decrease in T-2 toxin concentration, the SPR signal gradually increased. In other words, the SPR response was inversely proportional to T-2 toxin. The results showed that there was a good linear relationship in the range of 1 ng\/mL~100 ng\/mL, and the limit of detection was 0.57 ng\/mL. This work also provides a new possibility to improve the sensitivity of SPR biosensors in the detection of small molecules and in disease diagnosis.<\/jats:p>","DOI":"10.3390\/s23063078","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T03:04:46Z","timestamp":1678763086000},"page":"3078","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Surface Plasmon Resonance Sensor Based on Core-Shell Fe3O4@SiO2@Au Nanoparticles Amplification Effect for Detection of T-2 Toxin"],"prefix":"10.3390","volume":"23","author":[{"given":"Lirui","family":"Fan","sequence":"first","affiliation":[{"name":"School of Chemical Engineering, Sichuan University of Science and Engineering, Zigong 643000, China"},{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fubin","family":"Pei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijiao","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Tong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xihui","family":"Mu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyuan","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, Sichuan University of Science and Engineering, Zigong 643000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Thariani, R., and Yager, P. (2010). Imaging of surfaces by concurrent surface plasmon resonance and surface plasmon resonance-enhanced fluorescence. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009833"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"120202","DOI":"10.1016\/j.saa.2021.120202","article-title":"Femtomolar detection of dopamine using surface plasmon resonance sensor based on chitosan\/graphene quantum dots thin film","volume":"263","author":"Fen","year":"2021","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s11051-020-04872-0","article-title":"Improved the quality factor and sensitivity of a surface plasmon resonance sensor with transition metal dichalcogenide 2D nanomaterials","volume":"22","author":"Alagdar","year":"2020","journal-title":"J. Nanoparticle Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0250-6874(83)85036-7","article-title":"Surface Plasmon resonance for gas detection and biosensing","volume":"4","author":"Lundstrom","year":"1983","journal-title":"Sens. Actuators"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.talanta.2015.06.006","article-title":"SPR detection of cardiac troponin T for acute myocardial infarction","volume":"146","author":"Pawula","year":"2016","journal-title":"Talanta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.snb.2006.09.014","article-title":"Recent advancements in surface plasmon resonance immunosensors for detection of small molecules of biomedical, food and environmental interest","volume":"121","author":"Shankaran","year":"2007","journal-title":"Sens. Actuators B Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.snb.2014.08.055","article-title":"Fe3O4@Au nanoparticles enhanced surface plasmon resonance for ultrasensitive immunoassay","volume":"205","author":"Guo","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"214424","DOI":"10.1016\/j.ccr.2022.214424","article-title":"The performance enhancement of surface plasmon resonance optical sensors using nanomaterials: A review","volume":"458","author":"Philip","year":"2022","journal-title":"Coord. Chem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"120236","DOI":"10.1016\/j.saa.2021.120236","article-title":"Aspects of \u201cantigen-antibody\u201d interaction of chicken infectious bronchitis virus determined by surface plasmon resonance","volume":"264","author":"Klestova","year":"2022","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.snb.2014.03.041","article-title":"Application of an OLED integrated with BEF and giant birefringent optical (GBO) film in a SPR biosensor","volume":"198","author":"Prabowo","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.colsurfb.2014.10.037","article-title":"Sensitive and selective analysis of a wide concentration range of IGFBP7 using a surface plasmon resonance biosensor","volume":"123","author":"Jang","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1021\/acs.chemmater.6b05164","article-title":"Gold Nanoparticle\/Graphene Oxide Hybrid Sheets Attached on Mesenchymal Stem Cells for Effective Photothermal Cancer Therapy","volume":"29","author":"Kang","year":"2017","journal-title":"Chem. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s00604-019-3718-9","article-title":"A highly sensitive tetracycline sensor based on a combination of magnetic molecularly imprinted polymer nanoparticles and surface plasmon resonance detection","volume":"186","author":"Gao","year":"2019","journal-title":"Mikrochim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.snb.2017.07.061","article-title":"Magnetic nanoparticle enhanced surface plasmon resonance sensor for estradiol analysis","volume":"254","author":"Jia","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11944","DOI":"10.1021\/ac402848x","article-title":"Surface plasmon resonance sensor based on magnetic molecularly imprinted polymers amplification for pesticide recognition","volume":"85","author":"Yao","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"9251","DOI":"10.1021\/ac502271y","article-title":"First report of a direct surface plasmon resonance immunosensor for a small molecule seafood toxin","volume":"86","author":"Yakes","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.saa.2012.04.086","article-title":"A sensitive localized surface plasmon resonance sensor for determining mercury(II) ion using noble metal nanoparticles as probe","volume":"95","author":"Bi","year":"2012","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.snb.2015.02.062","article-title":"Fe3O4@Au nanoparticles as a means of signal enhancement in surface plasmon resonance spectroscopy for thrombin detection","volume":"212","author":"Chen","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.snb.2017.05.045","article-title":"U-bent fiber optic SPR sensor based on graphene\/AgNPs","volume":"251","author":"Zhang","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"18599","DOI":"10.1364\/OE.16.018599","article-title":"Surface plasmon resonance hydrogen sensor based on metallic grating with high sensitivity","volume":"16","author":"Lin","year":"2008","journal-title":"Opt. Express"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1002\/cbic.200400402","article-title":"SPR studies of carbohydrate-protein interactions: Signal enhancement of low-molecular-mass analytes by organoplatinum(II)-labeling","volume":"6","author":"Beccati","year":"2005","journal-title":"Chembiochem"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.talanta.2011.02.020","article-title":"Fe3O4 nanoparticles-enhanced SPR sensing for ultrasensitive sandwich bio-assay","volume":"84","author":"Wang","year":"2011","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.bios.2012.01.032","article-title":"Preparation and application of novel nanocomposites of magnetic-Au nanorod in SPR biosensor","volume":"34","author":"Zhang","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Grze\u015bkowiak, B.F., Tu\u015bnio, K., Wo\u017aniak, A., Szalata, M., Lipi\u0144ski, D., Jurga, S., and S\u0142omski, R. (2019). Transgenic Plant Detection Using an AuNPs Based SPR Biosensor. Biosensors, 9.","DOI":"10.3390\/bios9040116"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1007\/s12633-017-9608-z","article-title":"Synthesis of SPR Nanosensor using Gold Nanoparticles and its Application to Copper (II) Determination","volume":"10","author":"Deymehkar","year":"2017","journal-title":"Silicon"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.aca.2012.05.043","article-title":"Magnetic Fe3O4@Au composite-enhanced surface plasmon resonance for ultrasensitive detection of magnetic nanoparticle-enriched alpha-fetoprotein","volume":"737","author":"Liang","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3441","DOI":"10.1021\/jf200767q","article-title":"T-2 toxin, a trichothecene mycotoxin: Review of toxicity, metabolism, and analytical methods","volume":"59","author":"Li","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2018.10.307","article-title":"Monoclonal antibody production and the development of an indirect competitive enzyme-linked immunosorbent assay for screening T-2 toxin in milk","volume":"156","author":"Xu","year":"2018","journal-title":"Toxicon"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1039\/D1AY00642H","article-title":"A facile aptasensor based on polydopamine nanospheres for high-sensitivity sensing of T-2 toxin","volume":"13","author":"Guo","year":"2021","journal-title":"Anal. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wu, C., Wen, K., Jiang, H., Shen, J., Zhang, S., and Wang, Z. (2015). Comparison of Fluorescent Microspheres and Colloidal Gold as Labels in Lateral Flow Immunochromatographic Assays for the Detection of T-2 Toxin. Molecules, 21.","DOI":"10.3390\/molecules21010027"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"131523","DOI":"10.1016\/j.chemosphere.2021.131523","article-title":"Dong, Fast preparation of Fe3O4@polydopamine\/Au for highly efficient degradation of tetracycline","volume":"285","author":"Zhai","year":"2021","journal-title":"Chemosphere"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"14360","DOI":"10.1039\/C4NR04111A","article-title":"Multifunctional superparamagnetic nanoshells: Combining two-photon luminescence imaging, surface-enhanced Raman scattering and magnetic separation","volume":"6","author":"Jin","year":"2014","journal-title":"Nanoscale"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ab.2017.09.006","article-title":"Fluorescence and magnetic nanocomposite Fe3O4@SiO2@Au MNPs as peroxidase mimetics for glucose detection","volume":"538","author":"Luo","year":"2017","journal-title":"Anal. Biochem."},{"key":"ref_34","first-page":"1","article-title":"Tiwari, Gold Nanoparticles: Synthesis Methods, Functionalization and Biological Applications","volume":"4","author":"Patil","year":"2022","journal-title":"J. Clust. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bhandari, D., Chen, F.-C., and Bridgman, R.C. (2022). Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Romaine Lettuce. Sensors, 22.","DOI":"10.3390\/s22020475"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1021\/acs.langmuir.9b03869","article-title":"Femtomolar detection of spermidine using Au decorated SiO2 nanohybrid on plasmon-coupled extended cavity nanointerface: A smartphone-based fluorescence dequenching approach","volume":"36","author":"Bhaskar","year":"2020","journal-title":"Langmuir"},{"key":"ref_37","first-page":"13857","article-title":"Anisotropic Metal Nanoparticles: Synthesis, Assembly, and Optical Applications","volume":"36","author":"Murphy","year":"2010","journal-title":"J. Phys. Chem. B"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1002\/elan.201400237","article-title":"A High Sensitivity Electrochemical Sensor Based on Fe3+-Ion Molecularly Imprinted Film for the Detection of T-2 Toxin","volume":"26","author":"Gao","year":"2014","journal-title":"Electroanalysis"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.talanta.2009.12.055","article-title":"A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food","volume":"81","author":"Meneely","year":"2010","journal-title":"Talanta"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/6\/3078\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:53:52Z","timestamp":1760122432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/6\/3078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,13]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["s23063078"],"URL":"https:\/\/doi.org\/10.3390\/s23063078","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,13]]}}}