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However, the reflectance factors can affect the accuracy of predictions for chlorophyll and carotenoid concentrations. In this study, we tested the hypothesis that technology using two hyperspectral sensors for both reflectance and absorbance data would result in more accurate predictions of absorbance spectra. Our findings indicated that the green\/yellow regions (500\u2013600 nm) had a greater impact on photosynthetic pigment predictions, while the blue (440\u2013485 nm) and red (626\u2013700 nm) regions had a minor impact. Strong correlations were found between absorbance (R2 = 0.87 and 0.91) and reflectance (R2 = 0.80 and 0.78) for chlorophyll and carotenoids, respectively. Carotenoids showed particularly high and significant correlation coefficients using the partial least squares regression (PLSR) method (R2C = 0.91, R2cv = 0.85, and R2P = 0.90) when associated with hyperspectral absorbance data. Our hypothesis was supported, and these results demonstrate the effectiveness of using two hyperspectral sensors for optical leaf profile analysis and predicting the concentration of photosynthetic pigments using multivariate statistical methods. This method for two sensors is more efficient and shows better results compared to traditional single sensor techniques for measuring chloroplast changes and pigment phenotyping in plants.<\/jats:p>","DOI":"10.3390\/s23083843","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:24:18Z","timestamp":1681097058000},"page":"3843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["A Novel Method for Estimating Chlorophyll and Carotenoid Concentrations in Leaves: A Two Hyperspectral Sensor Approach"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2343-5045","authenticated-orcid":false,"given":"Renan","family":"Falcioni","sequence":"first","affiliation":[{"name":"Department of Agronomy, State University of Maringa, Av. Colombo, 5790, Maringa 87020-900, Parana, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8430-2791","authenticated-orcid":false,"given":"Werner Camargos","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Agronomy, State University of Maringa, Av. Colombo, 5790, Maringa 87020-900, Parana, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5328-0323","authenticated-orcid":false,"given":"Jos\u00e9 Alexandre Melo","family":"Dematt\u00ea","sequence":"additional","affiliation":[{"name":"Department of Soil Science, Luiz de Queiroz College of Agriculture, University of Sao Paulo, Av. Padua Dias, 11, Piracicaba 13418-260, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4854-2661","authenticated-orcid":false,"given":"Marcos Rafael","family":"Nanni","sequence":"additional","affiliation":[{"name":"Department of Agronomy, State University of Maringa, Av. Colombo, 5790, Maringa 87020-900, Parana, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1562\/0031-8655(2002)075<0272:ACCIPL>2.0.CO;2","article-title":"Assessing Carotenoid Content in Plant Leaves with Reflectance Spectroscopy","volume":"75","author":"Gitelson","year":"2002","journal-title":"Photochem. 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