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However, the differences between the vascular and blood-flow perfusion conditions under different levels of endurance-running performance remains unclear. The present study aimed to assess the vascular and blood-flow perfusion conditions among 3 groups (44 male volunteers) according to the time taken to run 3 km: Level 1, Level 2, and Level 3. Methods: The radial blood pressure waveform (BPW), finger photoplethygraphy (PPG), and skin-surface laser-Doppler flowmetry (LDF) signals of the subjects were measured. Frequency-domain analysis was applied to BPW and PPG signals; time- and frequency-domain analyses were applied to LDF signals. Results: Pulse waveform and LDF indices differed significantly among the three groups. These could be used to evaluate the advantageous cardiovascular effects provided by long-term endurance-running training, such as vessel relaxation (pulse waveform indices), improvement in blood supply perfusion (LDF indices), and changes in cardiovascular regulation activities (pulse and LDF variability indices). Using the relative changes in pulse-effect indices, we achieved almost perfect discrimination between Level 3 and Level 2 (AUC = 0.878). Furthermore, the present pulse waveform analysis could also be used to discriminate between the Level-1 and Level-2 groups. Conclusions: The present findings contribute to the development of a noninvasive, easy-to-use, and objective evaluation technique for the cardiovascular benefits of prolonged endurance-running training.<\/jats:p>","DOI":"10.3390\/s23083855","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:24:18Z","timestamp":1681097058000},"page":"3855","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Using Arterial Pulse and Laser Doppler Analyses to Discriminate between the Cardiovascular Effects of Different Running Levels"],"prefix":"10.3390","volume":"23","author":[{"given":"Yi-Jia","family":"Lin","sequence":"first","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Chien","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu-Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Chun","family":"Hsu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Family Medicine, Department of Family and Community Medicine, Tri-Service General Hospital, School of Medicine, National Defense Medical Center, Taipei 114, Taiwan"},{"name":"Health Management Center, Department of Family and Community Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-Chun","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"},{"name":"College of Applied Science, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6761-3774","authenticated-orcid":false,"given":"Hsin","family":"Hsiu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.jacc.2014.04.058","article-title":"Leisure-time running reduces all-cause and cardiovascular mortality risk","volume":"64","author":"Lee","year":"2014","journal-title":"J. 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