{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:05:03Z","timestamp":1771297503655,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Anhui Province\u2019s Major Science and Technology Special Projects","award":["202103a07020003"],"award-info":[{"award-number":["202103a07020003"]}]},{"name":"Anhui Province\u2019s Major Science and Technology Special Projects","award":["202003a06020018"],"award-info":[{"award-number":["202003a06020018"]}]},{"name":"Anhui Province\u2019s Major Science and Technology Special Projects","award":["202024a07020038"],"award-info":[{"award-number":["202024a07020038"]}]},{"name":"Anhui Province\u2019s Major Science and Technology Special Projects","award":["202104a06020005"],"award-info":[{"award-number":["202104a06020005"]}]},{"name":"Anhui Province\u2019s Major Science and Technology Special Projects","award":["X202210359867"],"award-info":[{"award-number":["X202210359867"]}]},{"name":"Anhui Provincial Key R&amp;D Programmes","award":["202103a07020003"],"award-info":[{"award-number":["202103a07020003"]}]},{"name":"Anhui Provincial Key R&amp;D Programmes","award":["202003a06020018"],"award-info":[{"award-number":["202003a06020018"]}]},{"name":"Anhui Provincial Key R&amp;D Programmes","award":["202024a07020038"],"award-info":[{"award-number":["202024a07020038"]}]},{"name":"Anhui Provincial Key R&amp;D Programmes","award":["202104a06020005"],"award-info":[{"award-number":["202104a06020005"]}]},{"name":"Anhui Provincial Key R&amp;D Programmes","award":["X202210359867"],"award-info":[{"award-number":["X202210359867"]}]},{"name":"Innovation and Entrepreneurship Training Program for Undergraduate of Hefei University of Technology","award":["202103a07020003"],"award-info":[{"award-number":["202103a07020003"]}]},{"name":"Innovation and Entrepreneurship Training Program for Undergraduate of Hefei University of Technology","award":["202003a06020018"],"award-info":[{"award-number":["202003a06020018"]}]},{"name":"Innovation and Entrepreneurship Training Program for Undergraduate of Hefei University of Technology","award":["202024a07020038"],"award-info":[{"award-number":["202024a07020038"]}]},{"name":"Innovation and Entrepreneurship Training Program for Undergraduate of Hefei University of Technology","award":["202104a06020005"],"award-info":[{"award-number":["202104a06020005"]}]},{"name":"Innovation and Entrepreneurship Training Program for Undergraduate of Hefei University of Technology","award":["X202210359867"],"award-info":[{"award-number":["X202210359867"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The present work developed an electrochemical genosensor for the detection of virulence outer membrane protein A (ompA, tDNA) gene of Cronobacter sakazakii (C. sakazakii) by exploiting the excellent glucose-oxidase-mimicking activity of copper Metal-organic frameworks (Cu-MOF) doped with gold nanoparticle (AuNPs). The signal nanotags of signal probes (sDNA) that biofunctionalized AuNPs@Cu-MOF (sDNA-AuNPs@Cu-MOF) were designed using an Au-S bond. The biosensor was prepared by immobilization capture probes (cDNA) onto an electrodeposited AuNPs-modified glassy carbon electrode (GCE). AuNPs@Cu-MOF was introduced onto the surface of the GCE via a hybridization reaction between cDNA and tDNA, as well as tDNA and sDNA. Due to the enhanced oxidase-mimicking activity of AuNPs@Cu-MOF to glucose, the biosensor gave a linear range of 1.0 \u00d7 10\u221215 to 1.0 \u00d7 10\u22129 mol L\u22121 to tDNA with a detection limit (LOD) of 0.42 fmol L\u22121 under optimized conditions using differential pulse voltammetry measurement (DPV). It can be applied in the direct detection of ompA gene segments in total DNA extracts from C. sakazakii with a broad linear range of 5.4\u22125.4 \u00d7 105 CFU mL\u22121 and a LOD of 0.35 CFU mL\u22121. The biosensor showed good selectivity, fabricating reproducibility and storage stability, and can be used for the detection of ompA gene segments in real samples with recovery between 87.5% and 107.3%.<\/jats:p>","DOI":"10.3390\/s23094396","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T12:12:11Z","timestamp":1682943131000},"page":"4396","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Electrochemical Detection of ompA Gene of C. sakazakii Based on Glucose-Oxidase-Mimicking Nanotags of Gold-Nanoparticles-Doped Copper Metal-organic Frameworks"],"prefix":"10.3390","volume":"23","author":[{"given":"Hongyan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"given":"Guiqing","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"given":"Yuming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Food Science, Xuancheng Campus, Hefei University of Technology, Xuancheng 242000, China"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"given":"Shaopeng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"given":"Feihao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Food Science, Xuancheng Campus, Hefei University of Technology, Xuancheng 242000, China"}]},{"given":"Pengyang","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Food Science, Xuancheng Campus, Hefei University of Technology, Xuancheng 242000, China"}]},{"given":"Chuanfu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Food Science, Xuancheng Campus, Hefei University of Technology, Xuancheng 242000, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1931-9049","authenticated-orcid":false,"given":"Xiaodong","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7703-8697","authenticated-orcid":false,"given":"Yongkang","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}]},{"given":"Yunlai","family":"Tao","sequence":"additional","affiliation":[{"name":"Anhui Institute of Food and Drug Inspection, Hefei 230051, China"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"534","DOI":"10.4315\/0362-028X.JFP-19-399","article-title":"Microbiological Quality of Powdered Infant Formula in Latin America","volume":"83","author":"Cerda","year":"2020","journal-title":"J. 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