{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:13:30Z","timestamp":1775063610674,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Padova"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Magnetic beads (or particles) having a size between 1 and 5 \u00b5m are largely used in many biochemical assays devoted to both purification and quantification of cells, nucleic acids, or proteins. Unfortunately, the use of these beads within microfluidic devices suffers from natural precipitation because of their size and density. The strategies applied thus far to cells or polymeric particles cannot be extended to magnetic beads, mainly due to their magnetization and their higher densities. We report an effective shaking device capable of preventing the sedimentation of beads that are stored in a custom PCR tube. After the characterization of the operating principle, the device is validated for magnetic beads in droplets, leading to an equal distribution between the droplets, barely affecting their generation.<\/jats:p>","DOI":"10.3390\/s23125399","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T02:02:28Z","timestamp":1686189748000},"page":"5399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Shaking Device for Homogeneous Dispersion of Magnetic Beads in Droplet Microfluidics"],"prefix":"10.3390","volume":"23","author":[{"given":"Maria","family":"Poles","sequence":"first","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-0767","authenticated-orcid":false,"given":"Alessio","family":"Meggiolaro","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6416-5782","authenticated-orcid":false,"given":"Sebastian","family":"Cremaschini","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"given":"Filippo","family":"Marinello","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9140-1528","authenticated-orcid":false,"given":"Daniele","family":"Filippi","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8973-0338","authenticated-orcid":false,"given":"Matteo","family":"Pierno","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3426-5475","authenticated-orcid":false,"given":"Giampaolo","family":"Mistura","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6760-7988","authenticated-orcid":false,"given":"Davide","family":"Ferraro","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1039\/c2lc21273k","article-title":"Microfluidic Approaches for Cancer Cell Detection, Characterization, and Separation","volume":"12","author":"Chen","year":"2012","journal-title":"Lab Chip"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.copbio.2019.11.022","article-title":"Microfluidic Systems for Cancer Diagnostics","volume":"65","author":"Maerkl","year":"2020","journal-title":"Curr. 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