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Taking the layered sediments (Stromatolite) formed by the growth of early life on the Earth as the research object, Raman spectroscopy is an essential means to detect deep-space extraterrestrial life. Fluorescence is the main factor that interferes with Raman spectroscopy detection, which will cause the enhancement of the Raman baseline and annihilate Raman information. The paper aims to evaluate fluorescence contained in the Raman baseline and characterize organic sedimentary structure using the Raman baseline. This study achieves spectral image fusion combined with mapping technology to obtain high spatial and spectral resolution fusion images. To clarify that the fluorescence of organic matter deposition is the main factor causing Raman baseline enhancement, 5041 Raman spectra were obtained in the scanning area of 710 \u03bcm \u00d7 710 \u03bcm, and the correlation mechanism between the gray level of the light-dark layer of the detection point and the Raman baseline was compared. The spatial distribution of carbonate minerals and organic precipitations was detected by combining mapping technology. In addition, based on the BI-IHS algorithm, the spectral image fusion of Raman fluorescence mapping and reflection micrograph, polarization micrograph, and orthogonal polarization micrograph are realized, respectively. A fusion image with high spectral resolution and high spatial resolution is obtained. The results show that the Raman baseline can be used as helpful information to characterize stromatolite organic sedimentary structure.<\/jats:p>","DOI":"10.3390\/s23136128","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T01:42:47Z","timestamp":1688434967000},"page":"6128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of Stromatolite Organic Sedimentary Structure Based on Spectral Image Fusion"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6801-2989","authenticated-orcid":false,"given":"Hongpeng","family":"Wang","sequence":"first","affiliation":[{"name":"College of Surveying and Geo-Informatics, Tongji University, Shanghai 200092, China"},{"name":"Key Laboratory of Space Active Opto-Electronics Technology of the Chinese Academy of Sciences, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinru","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjian","family":"Xin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Space Active Opto-Electronics Technology of the Chinese Academy of Sciences, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8753-992X","authenticated-orcid":false,"given":"Peipei","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yian","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1612-4844","authenticated-orcid":false,"given":"Sicong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Surveying and Geo-Informatics, Tongji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Space Active Opto-Electronics Technology of the Chinese Academy of Sciences, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Space Active Opto-Electronics Technology of the Chinese Academy of Sciences, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Wan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Space Active Opto-Electronics Technology of the Chinese Academy of Sciences, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China"},{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8739","DOI":"10.1002\/2017GL074480","article-title":"In situ detection of boron by ChemCam on Mars","volume":"44","author":"Gasda","year":"2017","journal-title":"Geophys. 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