{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T21:43:57Z","timestamp":1773351837013,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Foundation of Chongqing University of Technology","award":["2020ZDZ019"],"award-info":[{"award-number":["2020ZDZ019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Benefiting from the advantages like large surface area, flexible constitution, and diverse structure, metal-organic frameworks (MOFs) have been one of the most ideal candidates for nanozymes. In this study, a nitro-functionalized MOF, namely NO2-MIL-53(Cu), was synthesized. Multi-enzyme mimetic activities were discovered on this MOF, including peroxidase-like, oxidase-like, and laccase-like activity. Compared to the non-functional counterpart (MIL-53(Cu)), NO2-MIL-53(Cu) displayed superior enzyme mimetic activities, indicating a positive role of the nitro group in the MOF. Subsequently, the effects of reaction conditions on enzyme mimetic activities were investigated. Remarkably, NO2-MIL-53(Cu) exhibited excellent peroxidase-like activity even at neutral pH. Based on this finding, a simple colorimetric sensing platform was developed for the detection of H2O2 and glucose, respectively. The detection liner range for H2O2 is 1\u2013800 \u03bcM with a detection limit of 0.69 \u03bcM. The detection liner range for glucose is linear range 0.5\u2013300 \u03bcM with a detection limit of 2.6 \u03bcM. Therefore, this work not only provides an applicable colorimetric platform for glucose detection in a physiological environment, but also offers guidance for the rational design of efficient nanozymes with multi-enzyme mimetic activities.<\/jats:p>","DOI":"10.3390\/s23146277","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T01:58:14Z","timestamp":1689040694000},"page":"6277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Nitro Functionalized MOF with Multi-Enzyme Mimetic Activities for the Colorimetric Sensing of Glucose at Neutral pH"],"prefix":"10.3390","volume":"23","author":[{"given":"Ya","family":"Wang","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing University of Technology, Chongqing 400050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhua","family":"Wei","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing University of Technology, Chongqing 400050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing University of Technology, Chongqing 400050, China"},{"name":"Chongqing Institute of Innovation and Entrepreneurship for Precision Medicine, Chongqing 400050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3895-1744","authenticated-orcid":false,"given":"Guang","family":"Hu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing University of Technology, Chongqing 400050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113494","DOI":"10.1016\/j.bios.2021.113494","article-title":"A critical comparison of natural enzymes and nanozymes in biosensing and bioassays","volume":"192","author":"Ashrafi","year":"2021","journal-title":"Biosens. 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