{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:43:57Z","timestamp":1774496637667,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T00:00:00Z","timestamp":1689292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22064008"],"award-info":[{"award-number":["22064008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020010323"],"award-info":[{"award-number":["2020010323"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20210217-1"],"award-info":[{"award-number":["20210217-1"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Research and Development Program of Guilin","award":["22064008"],"award-info":[{"award-number":["22064008"]}]},{"name":"Key Research and Development Program of Guilin","award":["2020010323"],"award-info":[{"award-number":["2020010323"]}]},{"name":"Key Research and Development Program of Guilin","award":["20210217-1"],"award-info":[{"award-number":["20210217-1"]}]},{"name":"Scientific Research and Technology Development Plan of Guilin","award":["22064008"],"award-info":[{"award-number":["22064008"]}]},{"name":"Scientific Research and Technology Development Plan of Guilin","award":["2020010323"],"award-info":[{"award-number":["2020010323"]}]},{"name":"Scientific Research and Technology Development Plan of Guilin","award":["20210217-1"],"award-info":[{"award-number":["20210217-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The traditional lateral flow immunoassay (LFIA) detection method suffers from issues such as unstable detection results and low quantitative accuracy. In this study, we propose a novel multi-test line lateral flow immunoassay quantitative detection method using smartphone-based SAA immunoassay strips. Following the utilization of image processing techniques to extract and analyze the pigments on the immunoassay strips, quantitative analysis of the detection results was conducted. Experimental setups with controlled lighting conditions in a dark box were designed to capture samples using smartphones with different specifications for analysis. The algorithm\u2019s sensitivity and robustness were validated by introducing noise to the samples, and the detection performance on immunoassay strips using different algorithms was determined. The experimental results demonstrate that the proposed lateral flow immunoassay quantitative detection method based on image processing techniques achieves an accuracy rate of 94.23% on 260 samples, which is comparable to the traditional methods but with higher stability and lower algorithm complexity.<\/jats:p>","DOI":"10.3390\/s23146401","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T08:40:06Z","timestamp":1689324006000},"page":"6401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Quantitative Detection Algorithm for Multi-Test Line Lateral Flow Immunoassay Applied in Smartphones"],"prefix":"10.3390","volume":"23","author":[{"given":"Shenglan","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Advanced Manufacturing and Automation Technology (Guilin University of Technology), Education Department of Guangxi Zhuang Autonomous Region, Guilin 541006, China"}]},{"given":"Xincheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Advanced Manufacturing and Automation Technology (Guilin University of Technology), Education Department of Guangxi Zhuang Autonomous Region, Guilin 541006, China"}]},{"given":"Siqi","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Information Science and Engineering, Guilin University of Technology, Guilin 541006, China"}]},{"given":"Guangtian","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China"}]},{"given":"Shaojie","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Advanced Manufacturing and Automation Technology (Guilin University of Technology), Education Department of Guangxi Zhuang Autonomous Region, Guilin 541006, China"}]},{"given":"Liqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Advanced Manufacturing and Automation Technology (Guilin University of Technology), Education Department of Guangxi Zhuang Autonomous Region, Guilin 541006, China"}]},{"given":"Hongcheng","family":"Pan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105764","DOI":"10.1016\/j.pep.2020.105764","article-title":"Simultaneously targeting nitrocellulose and antibody by a dual-headed protein","volume":"177","author":"Tran","year":"2021","journal-title":"Protein Expr. 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