{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:05:32Z","timestamp":1769072732249,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"the Ministry of Science","doi-asserted-by":"publisher","award":["2021M3A9I5021439"],"award-info":[{"award-number":["2021M3A9I5021439"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"the Ministry of Science","doi-asserted-by":"publisher","award":["CRC22021-300"],"award-info":[{"award-number":["CRC22021-300"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"the Ministry of Science","doi-asserted-by":"publisher","award":["KGM9952314"],"award-info":[{"award-number":["KGM9952314"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008783","name":"the National Research Council of Science and Technology (NST)","doi-asserted-by":"publisher","award":["2021M3A9I5021439"],"award-info":[{"award-number":["2021M3A9I5021439"]}],"id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008783","name":"the National Research Council of Science and Technology (NST)","doi-asserted-by":"publisher","award":["CRC22021-300"],"award-info":[{"award-number":["CRC22021-300"]}],"id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008783","name":"the National Research Council of Science and Technology (NST)","doi-asserted-by":"publisher","award":["KGM9952314"],"award-info":[{"award-number":["KGM9952314"]}],"id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003715","name":"the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Initiative Research Program","doi-asserted-by":"publisher","award":["2021M3A9I5021439"],"award-info":[{"award-number":["2021M3A9I5021439"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003715","name":"the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Initiative Research Program","doi-asserted-by":"publisher","award":["CRC22021-300"],"award-info":[{"award-number":["CRC22021-300"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003715","name":"the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Initiative Research Program","doi-asserted-by":"publisher","award":["KGM9952314"],"award-info":[{"award-number":["KGM9952314"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Herein, we developed a bio-functionalized solution-immersed silicon (SIS) sensor at the single-cell level to identify Erwinia amylovora (E. amylovora), a highly infectious bacterial pathogen responsible for fire blight, which is notorious for its rapid spread and destructive impact on apple and pear orchards. This method allows for ultra-sensitive measurements without pre-amplification or labeling compared to conventional methods. To detect a single cell of E. amylovora, we used Lipopolysaccharide Transporter E (LptE), which is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane of E. amylovora, as a capture agent. We confirmed that LptE interacts with E. amylovora via LPS through in-house ELISA analysis, then used it to construct the sensor chip by immobilizing the capture molecule on the sensor surface modified with 3\u2032-Aminopropyl triethoxysilane (APTES) and glutaraldehyde (GA). The LptE-based SIS sensor exhibited the sensitive and specific detection of the target bacterial cell in real time. The dose\u2013response curve shows a linearity (R2 &gt; 0.992) with wide dynamic ranges from 1 to 107 cells\/mL for the target bacterial pathogen. The sensor showed the value change (d\u03a8) of approximately 0.008\u00b0 for growing overlayer thickness induced from a single-cell E. amylovora, while no change in the control bacterial cell (Bacillus subtilis) was observed, or negligible change, if any. Furthermore, the bacterial sensor demonstrated a potential for the continuous detection of E. amylovora through simple surface regeneration, enabling its reusability. Taken together, our system has the potential to be applied in fields where early symptoms are not observed and where single-cell or ultra-sensitive detection is required, such as plant bacterial pathogen detection, foodborne pathogen monitoring and analysis, and pathogenic microbial diagnosis.<\/jats:p>","DOI":"10.3390\/s23177400","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T08:42:20Z","timestamp":1692952940000},"page":"7400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor"],"prefix":"10.3390","volume":"23","author":[{"given":"Ui Jin","family":"Lee","sequence":"first","affiliation":[{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahang-ro, Yuseong-gu, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunkwang","family":"Oh","sequence":"additional","affiliation":[{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahang-ro, Yuseong-gu, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oh Seok","family":"Kwon","sequence":"additional","affiliation":[{"name":"SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Republic of Korea"},{"name":"Department of Nano Science and Technology, Sungkyunkwan University, Suwon 16419, Republic of Korea"},{"name":"Department of Nano Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3344-7875","authenticated-orcid":false,"given":"Jeong Mee","family":"Park","sequence":"additional","affiliation":[{"name":"Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahang-ro, Yuseong-gu, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Mo","family":"Cho","sequence":"additional","affiliation":[{"name":"Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moonil","family":"Kim","sequence":"additional","affiliation":[{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahang-ro, Yuseong-gu, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.femsle.2005.09.051","article-title":"Molecular genetics of Erwinia amylovora involved in the development of fire blight","volume":"253","author":"Oh","year":"2005","journal-title":"FEMS Microbiol. 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