{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:26:28Z","timestamp":1760149588529,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"state assignment of Rospotrebnadzor, Russia","award":["FWNR-2022-0024"],"award-info":[{"award-number":["FWNR-2022-0024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problems can be attributed to sensor design, while others can be attributed to technological limitations. The aim of this work is to conduct a theoretical investigation of the \u201cantibody + antigen\u201d complex (AB + AG) detection processes of a SOI-FET biosensor, which may also solve some of the aforementioned problems. Our investigation concentrates on the analysis of the probability of AB + AG complex detection and evaluation. Poisson probability density distribution was used to estimate the probability of the adsorption of the target molecules on the biosensor\u2019s surface and, consequently, to obtain correct detection results. Many implicit and unexpected causes of error detection have been identified for AB + AG complexes using SOI-FET biosensors. We showed that accuracy and time of detection depend on the number of SOI-FET biosensors on a crystal.<\/jats:p>","DOI":"10.3390\/s23177490","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T08:59:05Z","timestamp":1693299545000},"page":"7490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7544-187X","authenticated-orcid":false,"given":"Vladimir","family":"Generalov","sequence":"first","affiliation":[{"name":"Federal State Research Institution State Research Center of Virology and Biotechnology \u201cVector\u201d, 630559 Koltsovo, Russia"},{"name":"Faculty of Automation and Computer Engineering, Novosibirsk State Technical University, 630073 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9441-6450","authenticated-orcid":false,"given":"Anastasia","family":"Cheremiskina","sequence":"additional","affiliation":[{"name":"Federal State Research Institution State Research Center of Virology and Biotechnology \u201cVector\u201d, 630559 Koltsovo, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Glukhov","sequence":"additional","affiliation":[{"name":"Design Center of Bio-Microelectronic Technology Vega, 630082 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Grabezhova","sequence":"additional","affiliation":[{"name":"Design Center of Bio-Microelectronic Technology Vega, 630082 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0077-3823","authenticated-orcid":false,"given":"Margarita","family":"Kruchinina","sequence":"additional","affiliation":[{"name":"Research Institute of Internal and Preventive Medicine\u2014Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630089 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9161-6438","authenticated-orcid":false,"given":"Alexander","family":"Safatov","sequence":"additional","affiliation":[{"name":"Federal State Research Institution State Research Center of Virology and Biotechnology \u201cVector\u201d, 630559 Koltsovo, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.3906\/sag-2004-133","article-title":"Understanding dynamics of pandemics","volume":"50","author":"Akin","year":"2020","journal-title":"Turk. J. Med. Sci."},{"key":"ref_2","first-page":"36","article-title":"Highly pathogenic avian influenza\u2014A new pandemic threat and possibilities to resist it","volume":"12","author":"Onischenko","year":"2006","journal-title":"Vestn. Ross. Akad. Meditsinskikh Nauk."},{"key":"ref_3","unstructured":"WHO (2023, May 24). Coronavirus (COVID-19) Dashboard. Available online: https:\/\/covid19.who.int."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Maia, R., Carvalho, V., Faria, B., Miranda, I., Catarino, S., Teixeira, S., Lima, R., Minas, G., and Ribeiro, J. (2022). Diagnosis Methods for COVID-19: A Systematic Review. Micromachines, 13.","DOI":"10.3390\/mi13081349"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1373\/clinchem.2005.051532","article-title":"Enzyme Immunoassay (EIA)\/Enzyme-Linked Immunosorbent Assay (ELISA)","volume":"51","author":"Lequin","year":"2005","journal-title":"Clin. Chem."},{"key":"ref_6","unstructured":"Dolgov, V.V. (2015). Immunochemical Analysis in Laboratory Medicine, Triad. (In Russian)."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/s11901-021-00567-9","article-title":"Diagnostic Testing for SARS-CoV-2 Infection","volume":"20","author":"Thomas","year":"2021","journal-title":"Curr. Hepatol. Rep."},{"key":"ref_8","first-page":"227","article-title":"Factors affecting the antigen-antibody reaction","volume":"5","author":"Reverberi","year":"2007","journal-title":"Blood Transfus."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1109\/TBME.1970.4502688","article-title":"Development of an ion sensitive solid-state device for neurophysiological measurement","volume":"17","author":"Bergveld","year":"1970","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1109\/TBME.1972.324137","article-title":"Development, operation, and application of ion-sensitive field-effect transistor as a tool for electrophysiology","volume":"19","author":"Bergveld","year":"1972","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nbt1388","article-title":"Making it stick: Convection, reaction and diffusion in surface-based biosensors","volume":"26","author":"Squires","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/nnano.2012.82","article-title":"Quantification of the affinities and kinetics of protein interactions using silicon nanowire sensors","volume":"7","author":"Duan","year":"2012","journal-title":"Nat. Nanotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1021\/nl9034219","article-title":"Subthreshold regime has the optimal sensitivity for nanowire FET biosensors","volume":"10","author":"Gao","year":"2010","journal-title":"Nano Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"462","DOI":"10.18097\/PBMC20156104462","article-title":"SOI-nanowire biosensor for detection of D-NFAT 1 protein","volume":"61","author":"Malsagova","year":"2015","journal-title":"Biomeditsinskaya Khimiya"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3103\/S8756699016050034","article-title":"Optimization of the response of nanowire biosensors","volume":"52","author":"Naumova","year":"2015","journal-title":"Optoelectron. Instrum. Data Process."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Naumova, O., Generalov, V., Shcherbakov, D., Zaitseva, E., Zhivodkov, Y., Kozhukhov, A., Latyshev, A., Aseev, A., Safatov, A., and Buryak, G. (2022). SOI-FET sensors with dielectrophoretic concentration of viruses and proteins. Biosensors, 12.","DOI":"10.3390\/bios12110992"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Malsagova, K.A., Pleshakova, T.O., Kozlov, A.F., Galiullin, R.A., Popov, V.P., Tikhonenko, F.V., Glukhov, A.V., Ziborov, V.S., Shumov, I.D., and Petrov, O.F. (2021). Detection of influenza virus using a SOI-nanoribbon chip, based on an N-type field-effect transistor. Biosensors, 11.","DOI":"10.3390\/bios11040119"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2144\/000112071","article-title":"High-throughput multi-antigen microfluidic fluorescence immunoassays","volume":"40","author":"Kartalov","year":"2006","journal-title":"Biotechniques"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5135","DOI":"10.1021\/acsnano.0c02823","article-title":"Rapid detection of COVID-causative virus (SARS-CoV-2) in human nasopharyngeal swab specimens using field-effect transistor-based biosensor","volume":"14","author":"Seo","year":"2020","journal-title":"ACS Nano"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"100011","DOI":"10.1016\/j.cartre.2020.100011","article-title":"Graphene-based field-effect transistor biosensors for the rapid detection and analysis of viruses: A perspective in view of COVID-19","volume":"2","author":"Sengupta","year":"2021","journal-title":"Carbon Trends"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hu, W.P., Wu, Y.M., Vu, C.A., and Chen, W.Y. (2023). Ultrasensitive detection of interleukin 6 by using silicon nanowire field-effect transistors. Sensors, 23.","DOI":"10.3390\/s23020625"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1021\/nl0709017","article-title":"Surface charge sensitivity of silicon nanowires size dependence","volume":"7","author":"Juhasz","year":"2007","journal-title":"Nano Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.bios.2016.04.033","article-title":"Electrical detection of dengue virus (DENV) DNA oligomer using silicon nanowire biosensor with novel molecular gate control","volume":"83","author":"Nuzaihan","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_24","unstructured":"Landau, L.D., and Lifshitz, E.M. (1988). Theoretical Physics, Hydrodynamics, Nauka. [4th ed.]. (In Russian)."},{"key":"ref_25","unstructured":"Feynman, R., Leighton, R., and Sands, M. (1976). Feynman Lectures on Physics. Volume 4. Kinetics. Warmth. Sound, Mir. (In Russian)."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"6052","DOI":"10.1038\/s41467-022-33487-3","article-title":"Continuous biomarker monitoring with single molecule resolution by measuring free particle motion","volume":"13","author":"Buskermolen","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_27","unstructured":"Ermakov, S.M., and Zhiglyavsky, A.A. (1987). Mathematical Theory of Optimal Experiment. Studiesone Manual, Editorial Office of Physical, Mathematical and Technical Literature. (In Russian)."},{"key":"ref_28","unstructured":"Mushik, E., and Muller, P. (1990). Methods of Technical Decision-Making: Translated from German, Mir. (In Russian)."},{"key":"ref_29","unstructured":"Sachs, L. (1974). Statistische Auswertungsmethoden [Statistical Evaluation Methods], Springer."},{"key":"ref_30","unstructured":"Feller, W. (1984). Introduction to Probability Theory and Its Application, Mir. (In Russian)."},{"key":"ref_31","first-page":"62","article-title":"Preparation of the substrate surface of silicon nanowire field-effect transistors to create a biosensor","volume":"18","author":"Cheremiskina","year":"2021","journal-title":"Technol. Living Syst."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1080\/09603120020047519","article-title":"Decontamination issues for chemical and biological warfare agents: How clean is clean enough?","volume":"11","author":"Raber","year":"2001","journal-title":"Int. J. Environ. Health Res."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/17\/7490\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:41:33Z","timestamp":1760128893000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/23\/17\/7490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,29]]},"references-count":32,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["s23177490"],"URL":"https:\/\/doi.org\/10.3390\/s23177490","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2023,8,29]]}}}