{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T19:24:52Z","timestamp":1776281092278,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T00:00:00Z","timestamp":1704240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LH2023F046"],"award-info":[{"award-number":["LH2023F046"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["61905069"],"award-info":[{"award-number":["61905069"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LH2023F046"],"award-info":[{"award-number":["LH2023F046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61905069"],"award-info":[{"award-number":["61905069"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A novel label-free optical fiber biosensor, based on a microcavity fiber Mach\u2013Zehnder interferometer, was developed and practically demonstrated for DNA detection. The biosensor was fabricated using offset splicing standard communication single-mode fibers (SMFs). The light path of the sensor was influenced by the liquid sample in the offset open cavity. In the experiment, a high sensitivity of \u221217,905 nm\/RIU was achieved in the refractive index (RI) measurement. On this basis, the probe DNA (pDNA) was immobilized onto the sensor\u2019s surface using APTES, enabling real-time monitoring of captured complementary DNA (cDNA) samples. The experimental results demonstrate that the biosensor exhibited a high sensitivity of 0.32 nm\/fM and a limit of detection of 48.9 aM. Meanwhile, the sensor has highly repeatable and specific performance. This work reports an easy-to-manufacture, ultrasensitive, and label-free DNA biosensor, which has significant potential applications in medical diagnostics, bioengineering, gene identification, environmental science, and other biological fields.<\/jats:p>","DOI":"10.3390\/s24010278","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T03:18:07Z","timestamp":1704251887000},"page":"278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Label-Free DNA Hybridization Detection Using a Highly Sensitive Fiber Microcavity Biosensor"],"prefix":"10.3390","volume":"24","author":[{"given":"Yao","family":"Wu","sequence":"first","affiliation":[{"name":"College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-3922","authenticated-orcid":false,"given":"Xiujuan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China"},{"name":"Heilongjiang Provincial Key Laboratory of Metamaterials Physics and Device, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanji","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Metamaterials Physics and Device, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4824-8235","authenticated-orcid":false,"given":"Shengchun","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Metamaterials Physics and Device, Heilongjiang University, Harbin 150080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1093\/clinchem\/hvab274","article-title":"Error Characterization and Statistical Modeling Improves Circulating Tumor DNA Detection by Droplet Digital PCR","volume":"68","author":"Henriksen","year":"2022","journal-title":"Clin. 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