{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T07:52:05Z","timestamp":1773388325233,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["23KJ1570"],"award-info":[{"award-number":["23KJ1570"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["20H05886"],"award-info":[{"award-number":["20H05886"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["21H04663"],"award-info":[{"award-number":["21H04663"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JPMJCR22P6"],"award-info":[{"award-number":["JPMJCR22P6"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JPMJTR204C"],"award-info":[{"award-number":["JPMJTR204C"]}]},{"name":"CREST","award":["23KJ1570"],"award-info":[{"award-number":["23KJ1570"]}]},{"name":"CREST","award":["20H05886"],"award-info":[{"award-number":["20H05886"]}]},{"name":"CREST","award":["21H04663"],"award-info":[{"award-number":["21H04663"]}]},{"name":"CREST","award":["JPMJCR22P6"],"award-info":[{"award-number":["JPMJCR22P6"]}]},{"name":"CREST","award":["JPMJTR204C"],"award-info":[{"award-number":["JPMJTR204C"]}]},{"name":"JST A-STEP","award":["23KJ1570"],"award-info":[{"award-number":["23KJ1570"]}]},{"name":"JST A-STEP","award":["20H05886"],"award-info":[{"award-number":["20H05886"]}]},{"name":"JST A-STEP","award":["21H04663"],"award-info":[{"award-number":["21H04663"]}]},{"name":"JST A-STEP","award":["JPMJCR22P6"],"award-info":[{"award-number":["JPMJCR22P6"]}]},{"name":"JST A-STEP","award":["JPMJTR204C"],"award-info":[{"award-number":["JPMJTR204C"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Quantitative phase imaging by digital holographic microscopy (DHM) is a nondestructive and label-free technique that has been playing an indispensable role in the fields of science, technology, and biomedical imaging. The technique is competent in imaging and analyzing label-free living cells and investigating reflective surfaces. Herein, we introduce a new configuration of a wide field-of-view single-shot common-path off-axis reflective DHM for the quantitative phase imaging of biological cells that leverages several advantages, including being less-vibration sensitive to external perturbations due to its common-path configuration, also being compact in size, simple in optical design, highly stable, and cost-effective. A detailed description of the proposed DHM system, including its optical design, working principle, and capability for phase imaging, is presented. The applications of the proposed system are demonstrated through quantitative phase imaging results obtained from the reflective surface (USAF resolution test target) as well as transparent samples (living plant cells). The proposed system could find its applications in the investigation of several biological specimens and the optical metrology of micro-surfaces.<\/jats:p>","DOI":"10.3390\/s24030720","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T07:22:32Z","timestamp":1705994552000},"page":"720","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Live Cell Imaging by Single-Shot Common-Path Wide Field-of-View Reflective Digital Holographic Microscope"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-0677","authenticated-orcid":false,"given":"Manoj","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan"},{"name":"Center of Optical Scattering Image Science, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan"}]},{"given":"Takashi","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Applied Bioscience, Kanagawa Institute of Technology, Atsugi 243-0292, Japan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9767-0059","authenticated-orcid":false,"given":"Osamu","family":"Matoba","sequence":"additional","affiliation":[{"name":"Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan"},{"name":"Center of Optical Scattering Image Science, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"ref_1","first-page":"018005","article-title":"Principles and techniques of digital holographic microscopy","volume":"1","author":"Kim","year":"2010","journal-title":"SPIE Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1038\/s41566-018-0253-x","article-title":"Quantitative phase imaging in biomedicine","volume":"12","author":"Park","year":"2018","journal-title":"Nat. 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