{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:53:45Z","timestamp":1760144025948,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Sciences and Engineering Research Council of Canada (NSERC)","award":["RGPIN 2016-04943"],"award-info":[{"award-number":["RGPIN 2016-04943"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A transparent and adhesive film-based enclosing and sealing method is here presented for out-of-cleanroom-based open-form microfluidic devices. The commercially available polyester flexible film known as Microseal \u2018B\u2019 is presented in this paper as a cover seal for open-form microfluidic devices. This film is adaptable to high working temperatures and is biocompatible. The quality of the sealing film was investigated by leak tests, fluorescence tests, and contact angle measurements. The investigations revealed its sealing strength, fluorescence detection compatibility, and surface wettability. It was found that the proposed sealing polyester film on the 3D-printed device could sustain a gauge pressure of 2.7 atm at a flow rate of 4 mL\/min without any leaks. It also provided fluorescence detection compatibility and an intensity-to-background ratio in the range of 2.3 to 4.5 for particle sizes of 5 \u03bcm and 15 \u03bcm, respectively, which is comparable with the performances of other sealing materials. The film\u2019s hydrophobicity is comparable to other polymers used in microfluidics. This paper concludes by showcasing some applications of such transparent tops in classical microfluidic devices used for droplet generation and fluid mixing, in order to demonstrate the prospects of this fabrication technique in lab-on-a-chip devices.<\/jats:p>","DOI":"10.3390\/s24061797","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T04:51:12Z","timestamp":1710132672000},"page":"1797","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Demonstration of a Transparent and Adhesive Sealing Top for Microfluidic Lab-Chip Applications"],"prefix":"10.3390","volume":"24","author":[{"given":"Anurag","family":"Agarwal","sequence":"first","affiliation":[{"name":"MicroNano Mechatronics Laboratory, Department of Mechanical Automotive and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4, Canada"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2118-8842","authenticated-orcid":false,"given":"Asif","family":"Salahuddin","sequence":"additional","affiliation":[{"name":"MicroNano Mechatronics Laboratory, Department of Mechanical Automotive and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4, Canada"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3346-6187","authenticated-orcid":false,"given":"Mohammed Jalal","family":"Ahamed","sequence":"additional","affiliation":[{"name":"MicroNano Mechatronics Laboratory, Department of Mechanical Automotive and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4, Canada"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1021\/ac980656z","article-title":"Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)","volume":"70","author":"Duffy","year":"1998","journal-title":"Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1146\/annurev.matsci.28.1.153","article-title":"Soft Lithography","volume":"28","author":"Xia","year":"1998","journal-title":"Annu. Rev. Mater. Sci."},{"key":"ref_3","first-page":"4129","article-title":"Nanoimprint lithography","volume":"14","author":"Chou","year":"1996","journal-title":"J. Vac. Sci. Technol. B Microelectron. Nanometer Struct. Process. Meas. Phenom."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1063\/1.93501","article-title":"Natural lithography","volume":"41","author":"Deckman","year":"1982","journal-title":"Appl. Phys. Lett."},{"key":"ref_5","first-page":"2353","article-title":"Immersion lithography at 157 nm","volume":"19","author":"Switkes","year":"2001","journal-title":"J. Vac. Sci. Technol. B Microelectron. Nanometer Struct. Process. Meas. Phenom."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1073\/pnas.0435755100","article-title":"Gray-scale photolithography using microfluidic photomasks","volume":"100","author":"Chen","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1039\/c3lc41171k","article-title":"Direct laser writing of sub-50 nm nanofluidic channels buried in glass for three-dimensional micro-nanofluidic integration","volume":"13","author":"Liao","year":"2013","journal-title":"Lab Chip"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/s41378-022-00378-y","article-title":"Systematic characterization of cleanroom-free fabricated macrovalves, demonstrating pumps and mixers for automated fluid handling tuned for organ-on-chip applications","volume":"8","author":"Bossink","year":"2022","journal-title":"Microsyst. Nanoeng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1021\/ac9701997","article-title":"Microchannel Electrophoretic Separations of DNA in Injection-Molded Plastic Substrates | Analytical Chemistry","volume":"69","author":"McCormick","year":"1997","journal-title":"Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Gale, B.K., Jafek, A.R., Lambert, C.J., Goenner, B.L., Moghimifam, H., Nze, U.C., and Kamarapu, S.K. (2018). A Review of Current Methods in Microfluidic Device Fabrication and Future Commercialization Prospects. Inventions, 3.","DOI":"10.3390\/inventions3030060"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1039\/C6LC01430E","article-title":"3D printed metal molds for hot embossing plastic microfluidic devices","volume":"17","author":"Lin","year":"2017","journal-title":"Lab Chip"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"015036","DOI":"10.1088\/0960-1317\/20\/1\/015036","article-title":"Thermal assisted ultrasonic bonding of multilayer polymer microfluidic devices","volume":"20","author":"Zhang","year":"2009","journal-title":"J. Micromech. Microeng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1016\/j.jmatprotec.2012.06.022","article-title":"Fabrication of a thermoplastic multilayer microfluidic chip","volume":"212","author":"Li","year":"2012","journal-title":"J. Mater. Process. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.3390\/mi5041416","article-title":"Miscible Organic Solvents Soak Bonding Method Use in a PMMA Multilayer Microfluidic Device","volume":"5","author":"Zhang","year":"2014","journal-title":"Micromachines"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1039\/C6LC00163G","article-title":"The upcoming 3D-printing revolution in microfluidics","volume":"16","author":"Bhattacharjee","year":"2016","journal-title":"Lab Chip"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1039\/c1ay05253e","article-title":"Let there be chip\u2014Towards rapid prototyping of microfluidic devices: One-step manufacturing processes","volume":"3","author":"Waldbaur","year":"2011","journal-title":"Anal. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1126\/science.261.5123.895","article-title":"Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip","volume":"261","author":"Harrison","year":"1993","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1021\/ac00041a030","article-title":"Capillary electrophoresis and sample injection systems integrated on a planar glass chip","volume":"64","author":"Harrison","year":"1992","journal-title":"Anal. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0039-9140(01)00594-X","article-title":"Polymer microfluidic devices","volume":"56","author":"Becker","year":"2002","journal-title":"Talanta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3762","DOI":"10.1039\/C4LC00732H","article-title":"A polydimethylsiloxane\u2013polycarbonate hybrid microfluidic device capable of generating perpendicular chemical and oxygen gradients for cell culture studies","volume":"14","author":"Chang","year":"2014","journal-title":"Lab Chip"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"085008","DOI":"10.1088\/0960-1317\/25\/8\/085008","article-title":"Bonding PMMA microfluidics using commercial microwave ovens","volume":"25","author":"Toossi","year":"2015","journal-title":"J. Micromech. Microeng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3563","DOI":"10.1002\/elps.200305584","article-title":"Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies","volume":"24","author":"Sia","year":"2003","journal-title":"Electrophoresis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1021\/ar010110q","article-title":"Poly(dimethylsiloxane) as a Material for Fabricating Microfluidic Devices","volume":"35","author":"McDonald","year":"2002","journal-title":"Acc. Chem. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s10544-005-6070-2","article-title":"Characterization of Polydimethylsiloxane (PDMS) Properties for Biomedical Micro\/Nanosystems","volume":"7","author":"Mata","year":"2005","journal-title":"Biomed. Microdevices"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1146\/annurev-anchem-091619-102649","article-title":"3D Printed Microfluidics","volume":"13","author":"Nielsen","year":"2020","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1002\/elan.201600043","article-title":"Developments of 3D Printing Microfluidics and Applications in Chemistry and Biology: A Review","volume":"28","author":"He","year":"2016","journal-title":"Electroanalysis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3240","DOI":"10.1021\/ac403397r","article-title":"Spence Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences","volume":"86","author":"Gross","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"116892","DOI":"10.1016\/j.trac.2022.116892","article-title":"3D printed microfluidics for bioanalysis: A review of recent advancements and applications","volume":"158","author":"Kitiara","year":"2023","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1039\/c4lc00320a","article-title":"Print your own membrane: Direct rapid prototyping of polydimethylsiloxane","volume":"14","author":"Femmer","year":"2014","journal-title":"Lab Chip"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7620","DOI":"10.1002\/anie.201900530","article-title":"3D-Printing of Functionally Graded Porous Materials Using On-Demand Reconfigurable Microfluidics","volume":"58","author":"Costantini","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Goh, W.H., and Hashimoto, M. (2018). Dual Sacrificial Molding: Fabricating 3D Microchannels with Overhang and Helical Features. Micromachines, 9.","DOI":"10.3390\/mi9100523"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1002\/adma.201500222","article-title":"Microfluidic Printheads for Multimaterial 3D Printing of Viscoelastic Inks","volume":"27","author":"Hardin","year":"2015","journal-title":"Adv. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1126\/science.aaa2397","article-title":"Continuous liquid interface production of 3D objects","volume":"347","author":"Tumbleston","year":"2015","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1039\/C5LC00126A","article-title":"3D-printed microfluidic automation","volume":"15","author":"Au","year":"2015","journal-title":"Lab Chip"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rogers, C.I., Qaderi, K., Woolley, A.T., and Nordin, G.P. (2015). 3D printed microfluidic devices with integrated valves. Biomicrofluidics, 9.","DOI":"10.1063\/1.4905840"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Aghaseyedi, M., Salehi, A., Valijam, S., and Shooshtari, M. (2022). Gas Selectivity Enhancement Using Serpentine Microchannel Shaped with Optimum Dimensions in Microfluidic-Based Gas Sensor. Micromachines, 13.","DOI":"10.3390\/mi13091504"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.1002\/adma.201305506","article-title":"3D Bioprinting of Vascularized, Heterogeneous Cell-Laden Tissue Constructs","volume":"26","author":"Kolesky","year":"2014","journal-title":"Adv. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1208\/s12249-022-02242-8","article-title":"3D Printing: Applications in Tissue Engineering, Medical Devices, and Drug Delivery","volume":"23","author":"Kumar","year":"2022","journal-title":"AAPS Pharm. Sci. Tech."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zhang, Q., Bei, H.P., Zhao, M., Dong, Z., and Zhao, X. (2022). Shedding light on 3D printing: Printing photo-crosslinkable constructs for tissue engineering. Biomaterials, 286.","DOI":"10.1016\/j.biomaterials.2022.121566"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nchem.1313","article-title":"Integrated 3D-printed reactionware for chemical synthesis and analysis","volume":"4","author":"Symes","year":"2012","journal-title":"Nat. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3267","DOI":"10.1039\/c2lc40761b","article-title":"Configurable 3D-Printed millifluidic and microfluidic \u2018lab on a chip\u2019 reactionware devices","volume":"12","author":"Kitson","year":"2012","journal-title":"Lab Chip"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14701","DOI":"10.1021\/acs.iecr.1c03098","article-title":"Enhanced Fluid Dynamics in 3D Monolithic Reactors to Improve the Chemical Performance: Experimental and Numerical Investigation","volume":"60","author":"Quintanilla","year":"2021","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1039\/C4LC00171K","article-title":"3D printed microfluidic devices with integrated versatile and reusable electrodes","volume":"14","author":"Erkal","year":"2014","journal-title":"Lab Chip"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1604827","DOI":"10.1002\/adma.201604827","article-title":"3D Printing of Transparent and Conductive Heterogeneous Hydrogel\u2013Elastomer Systems","volume":"29","author":"Tian","year":"2017","journal-title":"Adv. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1039\/C6LC00153J","article-title":"3D-printing of transparent bio-microfluidic devices in PEG-DA","volume":"16","author":"Urrios","year":"2016","journal-title":"Lab Chip"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.proeng.2010.09.105","article-title":"3D Rapid Prototyping Technology (RPT) as a powerful tool in microfluidic development","volume":"5","author":"Ring","year":"2010","journal-title":"Procedia Eng."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1039\/C4LC00394B","article-title":"Low cost lab-on-a-chip prototyping with a consumer grade 3D printer","volume":"14","author":"Comina","year":"2014","journal-title":"Lab Chip"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1007\/s00542-017-3528-0","article-title":"Fully enclosed paper-based microfluidic devices using bio-compatible adhesive seals","volume":"24","author":"Fan","year":"2018","journal-title":"Microsyst. Technol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3699","DOI":"10.1021\/ac800112r","article-title":"Simple Telemedicine for Developing Regions: Camera Phones and Paper-Based Microfluidic Devices for Real-Time, Off-Site Diagnosis","volume":"80","author":"Martinez","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3284","DOI":"10.1016\/j.bios.2010.12.044","article-title":"A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for uric acid determination","volume":"26","author":"Yu","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Yu, P., Deng, M., and Yang, Y. (2019). New Single-Layered Paper-Based Microfluidic Devices for the Analysis of Nitrite and Glucose Built via Deposition of Adhesive Tape. Sensors, 19.","DOI":"10.3390\/s19194082"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4824","DOI":"10.1038\/s41598-019-41208-y","article-title":"Reconfigurable Acrylic-tape Hybrid Microfluidics","volume":"9","author":"Ren","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s10404-018-2162-1","article-title":"Rapid prototyping of shrinkable BOPS-based microfluidic devices","volume":"22","author":"Fan","year":"2018","journal-title":"Microfluid. Nanofluid."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1007\/s00542-017-3630-3","article-title":"Rapid prototyping of flexible multilayer microfluidic devices using polyester sealing film","volume":"24","author":"Fan","year":"2018","journal-title":"Microsyst. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Boos, J.A., and Beuvink, I. (2016). Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents, Springer.","DOI":"10.1007\/978-1-4939-3148-4_8"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chen, X., Hou, S., Chu, J., Xiong, Y., Xiong, P., Liu, G., and Tian, Y. (2017). Observation Interface of PDMS Membrane in a Microfluidic Chip Based on One-Step Molding. Micromachines, 8.","DOI":"10.3390\/mi8030064"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"122106","DOI":"10.1063\/1.5054898","article-title":"Wettability-mediated dynamics of two-phase flow in microfluidic T-junction","volume":"30","author":"Boruah","year":"2018","journal-title":"Phys. Fluids"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1039\/C3LC51406D","article-title":"Wettability patterning for high-rate, pumpless fluid transport on open, non-planar microfluidic platforms\u2014Lab on a Chip (RSC Publishing)","volume":"14","author":"Ghosh","year":"2014","journal-title":"Lab Chip"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1063\/1.1499125","article-title":"Displacement of a two-dimensional immiscible droplet in a channel","volume":"14","author":"Kang","year":"2002","journal-title":"Phys. Fluids"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1017\/S0022112005006956","article-title":"Displacement of a three-dimensional immiscible droplet in a duct","volume":"545","author":"Kang","year":"2005","journal-title":"J. Fluid Mech."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1080\/01694243.2016.1142799","article-title":"Uncertainty in contact angle measurements from the tangent method","volume":"30","author":"Extrand","year":"2016","journal-title":"J. Adhes. Sci. Technol."},{"key":"ref_62","first-page":"650","article-title":"Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices","volume":"13","author":"Camilo","year":"2022","journal-title":"Micromechanics"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"016601","DOI":"10.1088\/0034-4885\/75\/1\/016601","article-title":"Droplet based microfluidics","volume":"75","author":"Seemann","year":"2011","journal-title":"Rep. Prog. Phys."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Werther, M., and Seitz, H. (2008). Lab-on-a-Chip In Vitro Compartmentalization Technologies for Protein Studies Protein\u2014Protein Interaction Advances in Biochemical Engineering\/Biotechnology, Springer.","DOI":"10.1007\/978-3-540-68820-4"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1002\/adma.200803386","article-title":"The Synthesis and Assembly of Polymeric Microparticles Using Microfluidics","volume":"21","author":"Dendukuri","year":"2009","journal-title":"Adv. Mater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5846","DOI":"10.1002\/anie.200906653","article-title":"Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology","volume":"49","author":"Theberge","year":"2010","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"035013","DOI":"10.1088\/0960-1317\/25\/3\/035013","article-title":"Experimental validation of a simple, low-cost, T-junction droplet generator fabricated through 3D printing","volume":"25","author":"Donvito","year":"2015","journal-title":"J. Micromech. Microeng."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1007\/s00542-020-04787-9","article-title":"Design and development of an efficient fluid mixing for 3D printed lab-on-a-chip","volume":"26","author":"Agarwal","year":"2020","journal-title":"Microsyst. Technol."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/6\/1797\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:11:51Z","timestamp":1760105511000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/6\/1797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,11]]},"references-count":68,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["s24061797"],"URL":"https:\/\/doi.org\/10.3390\/s24061797","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2024,3,11]]}}}