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During the synthetic process, gold nanoparticles were loaded onto the active sites of nitrogen-doped graphene fibers. Then, aptamers were modified on the microelectrode depending on Au-S bonds to prepare Apt-Au-N-RGOF. The prepared microelectrode can specifically identify DA, avoiding interference with other molecules and improving its selectivity. Compared with the N-RGOF microelectrode, the Apt-Au-N-RGOF microelectrode exhibited higher sensitivity, a lower detection limit (0.5 \u03bcM), and a wider linear range (1~100 \u03bcM) and could be applied in electrochemical analysis fields.<\/jats:p>","DOI":"10.3390\/s24092934","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T14:26:11Z","timestamp":1715005571000},"page":"2934","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Dopamine Based on Aptamer-Modified Graphene Microelectrode"],"prefix":"10.3390","volume":"24","author":[{"given":"Cuicui","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Science, China University of Geosciences (Beijing), Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyou","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Science, China University of Geosciences (Beijing), Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiran","family":"Ying","sequence":"additional","affiliation":[{"name":"School of Science, China University of Geosciences (Beijing), Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5954-2793","authenticated-orcid":false,"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Science, China University of Geosciences (Beijing), Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/acsnanoscienceau.2c00039","article-title":"Significance of an Electrochemical Sensor and Nanocomposites: Toward the Electrocatalytic Detection of Neurotransmitters and Their Importance within the Physiological System","volume":"3","author":"Kaur","year":"2022","journal-title":"ACS Nanosci. 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B-Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.talanta.2019.03.096","article-title":"A Novel Electrochemical Sensor Based on Carbon Nanotubes Array for Selective Detection of Dopamine or Uric Acid","volume":"201","author":"Yang","year":"2019","journal-title":"Talanta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107038","DOI":"10.1016\/j.solidstatesciences.2022.107038","article-title":"Polypyrrole-Derived Carbon Nanotubes for Potential Application in Electrochemical Detection of Dopamine","volume":"134","author":"Wang","year":"2022","journal-title":"Solid State Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e202200330","DOI":"10.1002\/celc.202200330","article-title":"Comparison of Carbon Nanomaterials for Simultaneous Detection of Neurotransmitters in the Presence of Interfering Species","volume":"9","author":"Mazurkiewicz","year":"2022","journal-title":"ChemElectroChem"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"110360","DOI":"10.1016\/j.dyepig.2022.110360","article-title":"Synthesis of a Sulfonated Methylene Blue-Backboned Polymer for Biodetections","volume":"203","author":"Cai","year":"2022","journal-title":"Dyes Pigm."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Gu\u0163oiu, S., Pog\u0103cean, F., M\u0103geru\u015fan, L., Micl\u0103u\u015f, M.O., Grad, O., Pan\u0103, I.-O., and Pruneanu, S. (2023). Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide. 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Electroanal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"130926","DOI":"10.1016\/j.matlet.2021.130926","article-title":"Palladium Nanoparticles Decorated Ni-MOF Nanocomposite as an Electrochemical Platform for the Selective Detection of Dopamine","volume":"306","author":"Kavya","year":"2022","journal-title":"Mater. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1007\/s11581-020-03857-2","article-title":"Electrochemical Sensing of Dopamine Using a Ni-Based Metal-Organic Framework Modified Electrode","volume":"27","author":"Huang","year":"2021","journal-title":"Ionics (Kiel)"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"107012","DOI":"10.1016\/j.elecom.2021.107012","article-title":"NO2-Functionalized Metal-Organic Framework Incorporating Bimetallic Alloy Nanoparticles as a Sensor for Efficient Electrochemical Detection of Dopamine","volume":"125","author":"Hira","year":"2021","journal-title":"Electrochem. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"140586","DOI":"10.1016\/j.chemosphere.2023.140586","article-title":"An Inexpensive Paracetamol Sensor Based on an Acid-Activated Carbon Fiber Microelectrode","volume":"346","year":"2024","journal-title":"Chemosphere"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1805867","DOI":"10.1002\/adma.201805867","article-title":"High-Performance Graphene-Fiber-Based Neural Recording Microelectrodes","volume":"31","author":"Wang","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"18996","DOI":"10.1002\/anie.202008284","article-title":"A Generalizable and Noncovalent Strategy for Interfacing Aptamers with a Microelectrode for the Selective Sensing of Neurotransmitters in\u2005Vivo","volume":"59","author":"Hou","year":"2020","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"100957","DOI":"10.1016\/j.coelec.2022.100957","article-title":"Recent Progress in Improving the Performance of In Vivo Electrochemical Microsensor Based on Materials","volume":"33","author":"Zeng","year":"2022","journal-title":"Curr. Opin. Electrochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"33844","DOI":"10.1039\/D3RA06218J","article-title":"Simultaneous Detection of Neurotransmitters and Cu2+ Using Double-Bore Carbon Fiber Microelectrodes Via Fast-Scan Cyclic Voltammetry","volume":"13","author":"Manring","year":"2023","journal-title":"RSC Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3266","DOI":"10.1016\/j.arabjc.2018.11.002","article-title":"Carbon Nanotube\/Carbon Fiber Electrodes Via Chemical Vapor Deposition for Simultaneous Determination of Ascorbic Acid, Dopamine and Uric Acid","volume":"13","author":"Zhao","year":"2020","journal-title":"Arabian J. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4803","DOI":"10.1021\/acs.analchem.1c05637","article-title":"Graphene-Fiber Microelectrodes for Ultrasensitive Neurochemical Detection","volume":"94","author":"Li","year":"2022","journal-title":"Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1021\/acssensors.9b01583","article-title":"Facile Development of a Fiber-Based Electrode for Highly Selective and Sensitive Detection of Dopamine","volume":"4","author":"Farajikhah","year":"2019","journal-title":"ACS Sens."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4632","DOI":"10.1039\/D2TB00906D","article-title":"Graphene-Based Implantable Neural Electrodes for Insect Flight Control","volume":"10","author":"Xiong","year":"2022","journal-title":"J. Mater. Chem. B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.snb.2018.06.039","article-title":"Multi-Element Doping Design of High-Efficient Carbocatalyst for Electrochemical Sensing of Cancer Cells","volume":"273","author":"Xi","year":"2018","journal-title":"Sens. Actuat. B-Chem."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hsine, Z., Mlika, R., Jaffrezic-Renault, N., and Korri-Youssoufi, H. (2022). Review-Recent Progress in Graphene Based Modified Electrodes for Electrochemical Detection of Dopamine. Chemosensors, 10.","DOI":"10.3390\/chemosensors10070249"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.elecom.2016.09.021","article-title":"A Novel Nitrogen-Doped Graphene Fiber Microelectrode with Ultrahigh Sensitivity for the Detection of Dopamine","volume":"72","author":"Ding","year":"2016","journal-title":"Electrochem. Commun."},{"key":"ref_30","first-page":"e00221","article-title":"Electrospun Nitrogen-Doped Carbon Nanofibers for Electrocatalysis","volume":"26","author":"Lu","year":"2020","journal-title":"SM&T"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"129683","DOI":"10.1016\/j.snb.2021.129683","article-title":"Green and Controllable Synthesis of Multi-Heteroatoms Co-Doped Graphene Fiber as Flexible and Biocompatible Microelectrode for in Situ Electrochemical Detection of Biological Samples","volume":"335","author":"Zhao","year":"2021","journal-title":"Sens. Actuat. B-Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"155655","DOI":"10.1016\/j.apsusc.2022.155655","article-title":"3D Heteroatom-Doped Graphene-Wrapped Flexible Carbon Fiber Microsensor for Real-Time Hydrogen Peroxide Detection in Live Cancer Cells","volume":"611","author":"Zhang","year":"2023","journal-title":"Appl. Surf. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.talanta.2019.03.007","article-title":"A Chemiluminescence Biosensor for Lysozyme Detection Based on Aptamers and Hemin\/G-Quadruplex Dnazyme Modified Sandwich-Rod Carbon Fiber Composite","volume":"200","author":"Sun","year":"2019","journal-title":"Talanta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"109927","DOI":"10.1016\/j.microc.2024.109927","article-title":"Electrochemical Biosensor for the Detection of Low Density Lipoprotein Based on Gold Nanoparticles Mediated Bi-Enzymes Catalytic Silver Deposition Reaction","volume":"199","author":"Luo","year":"2024","journal-title":"Microchem. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1109\/TIM.2023.3284027","article-title":"Determination of Phenylalanine by a Novel Enzymatic PHD\/SPE Biosensor","volume":"72","author":"Ferlazzo","year":"2023","journal-title":"IEEE Trans. Instrum. Meas."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"065502","DOI":"10.1088\/1361-6528\/abc388","article-title":"Flexible and Disposable Gold Nanoparticles-N-Doped Carbon-Modified Electrochemical Sensor for Simultaneous Detection of Dopamine and Uric Acid","volume":"32","author":"Patil","year":"2020","journal-title":"Nanotechnology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3772","DOI":"10.1038\/s41598-020-60715-x","article-title":"High-Performance Conducting Polymer Nanotube-Based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis","volume":"10","author":"Park","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_38","first-page":"973","article-title":"Measurement of Neuropeptide Y Using Aptamer-Modified Microelectrodes by Electrochemical Impedance Spectroscopy","volume":"93","author":"Cruz","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"133105","DOI":"10.1016\/j.foodchem.2022.133105","article-title":"Ultrasensitive Aptasensor Using Electrospun Mxene\/Polyvinylidene Fluoride Nanofiber Composite for Ochratoxin a Detection","volume":"390","author":"Jose","year":"2022","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Nguyen, N.V., and Jen, C. (2019). Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles. Micromachines, 10.","DOI":"10.3390\/mi10030195"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6828","DOI":"10.1021\/acs.analchem.2c05335","article-title":"Selective Aptamer Modification of Au Surfaces in a Microelectrode Sensor Array for Simultaneous Detection of Multiple Analytes","volume":"95","author":"Sen","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5856","DOI":"10.1021\/ja800745y","article-title":"Flexible Graphene Films Via the Filtration of Water-Soluble Noncovalent Functionalized Graphene Sheets","volume":"130","author":"Xu","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.apsusc.2018.10.220","article-title":"Electrospun Nitrogen-Doped Carbon Nanofiber as Negative Electrode for Vanadium Redox Flow Battery","volume":"469","author":"He","year":"2019","journal-title":"Appl. Surf. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1016\/j.carbon.2008.06.027","article-title":"Surface Functional Groups of Carbons and the Effects of Their Chemical Character, Density and Accessibility to Ions on Electrochemical Performance","volume":"46","author":"Seredych","year":"2008","journal-title":"Carbon"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1039\/C3NR04714H","article-title":"In Situ Growth of Au Nanocrystals on Graphene Oxide Sheets","volume":"6","author":"Qin","year":"2014","journal-title":"Nanoscale"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1016\/j.arabjc.2017.12.021","article-title":"One-Step Synthesis of Reduced Graphene Oxide\/Gold Nanoparticles under Ambient Conditions","volume":"13","year":"2020","journal-title":"Arab. J. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"157816","DOI":"10.1016\/j.jallcom.2020.157816","article-title":"Study on Preparation and Performance of Flexible All-Solid-State Supercapacitor Based on Nitrogen-Doped RGO\/CNT\/MnO2 Composite Fibers","volume":"859","author":"Zhang","year":"2021","journal-title":"J. Alloys Compd."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.carbon.2018.01.033","article-title":"High Rate Integrated Quasi-Solid State Supercapacitors Based on Nitrogen-Enriched Active Carbon Fiber\/Reduced Graphene Oxide Nanocomposite","volume":"130","author":"Qiu","year":"2018","journal-title":"Carbon"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/9\/2934\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:39:55Z","timestamp":1760107195000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/9\/2934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,5]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["s24092934"],"URL":"https:\/\/doi.org\/10.3390\/s24092934","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,5]]}}}