{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T01:07:50Z","timestamp":1786237670668,"version":"3.56.0"},"reference-count":16,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020","award":["874864 HEDIMED"],"award-info":[{"award-number":["874864 HEDIMED"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>With the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergen-specific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 \u03bcL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for large-scale research in allergies.<\/jats:p>","DOI":"10.3390\/s24113280","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T07:56:11Z","timestamp":1716364571000},"page":"3280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Point-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2676-9785","authenticated-orcid":false,"given":"Silvia","family":"Demuru","sequence":"first","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Chai-Gao","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1587-1752","authenticated-orcid":false,"given":"Yevhen","family":"Shynkarenko","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Hermann","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia-Daiana","family":"Boia","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-1824","authenticated-orcid":false,"given":"Peter","family":"Cristofolini","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley","family":"Petkus","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Generelli","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Paoletti","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6140-0364","authenticated-orcid":false,"given":"Stefano","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6068-709X","authenticated-orcid":false,"given":"Lo\u00efc","family":"Burr","sequence":"additional","affiliation":[{"name":"Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"0300060520903220","DOI":"10.1177\/0300060520903220","article-title":"Exosomes: An Important Messenger in the Asthma Inflammatory Microenvironment","volume":"48","author":"Huang","year":"2020","journal-title":"J. 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