{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:03:06Z","timestamp":1776747786187,"version":"3.51.2"},"reference-count":111,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Projects of Chongqing Natural Science Foundation","award":["CSTB2024NSCQ-MSX0247"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0247"]}]},{"name":"General Projects of Chongqing Natural Science Foundation","award":["2022YFB3205600"],"award-info":[{"award-number":["2022YFB3205600"]}]},{"name":"General Projects of Chongqing Natural Science Foundation","award":["12241203"],"award-info":[{"award-number":["12241203"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["CSTB2024NSCQ-MSX0247"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0247"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFB3205600"],"award-info":[{"award-number":["2022YFB3205600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["12241203"],"award-info":[{"award-number":["12241203"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CSTB2024NSCQ-MSX0247"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0247"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022YFB3205600"],"award-info":[{"award-number":["2022YFB3205600"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12241203"],"award-info":[{"award-number":["12241203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Saccharides, being one of the fundamental molecules of life, play essential roles in the physiological and pathological functions of cells. However, their intricate structures pose challenges for detection. Nanopore technology, with its high sensitivity and capability for single-molecule-level analysis, has revolutionized the identification and structural analysis of saccharide molecules. This review focuses on recent advancements in nanopore technology for carbohydrate detection, presenting an array of methods that leverage the molecular complexity of saccharides. Biological nanopore techniques utilize specific protein binding or pore modifications to trigger typical resistive pulses, enabling the high-sensitivity detection of monosaccharides and oligosaccharides. In solid-state nanopore sensing, boronic acid modification and pH gating mechanisms are employed for the specific recognition and quantitative analysis of polysaccharides. The integration of artificial intelligence algorithms can further enhance the accuracy and reliability of analyses. Serving as a crucial tool in carbohydrate detection, we foresee significant potential in the application of nanopore technology for the detection of carbohydrate molecules in disease diagnosis, drug screening, and biosensing, fostering innovative progress in related research domains.<\/jats:p>","DOI":"10.3390\/s24165442","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T12:58:07Z","timestamp":1724417887000},"page":"5442","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Research Progress on Saccharide Molecule Detection Based on Nanopores"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2499-9969","authenticated-orcid":false,"given":"Bohua","family":"Yin","sequence":"first","affiliation":[{"name":"International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China"},{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3305-043X","authenticated-orcid":false,"given":"Wanyi","family":"Xie","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5515-6393","authenticated-orcid":false,"given":"Shaoxi","family":"Fang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2192-7177","authenticated-orcid":false,"given":"Shixuan","family":"He","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Ma","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"},{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3990-064X","authenticated-orcid":false,"given":"Liyuan","family":"Liang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajie","family":"Yin","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7056-597X","authenticated-orcid":false,"given":"Daming","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuobin","family":"Wang","sequence":"additional","affiliation":[{"name":"International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China"},{"name":"Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Guo, Z. (2024). Introduction to Carbohydrates and Glycoconjugates. Glycosphingolipids in the Central Nervous System, Elsevier.","DOI":"10.1016\/B978-0-443-16156-8.00001-8"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/j.clinbiochem.2013.04.027","article-title":"A Quick Look at Biochemistry: Carbohydrate Metabolism","volume":"46","author":"Dashty","year":"2013","journal-title":"Clin. Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zeng, Y., Himmel, M.E., and Ding, S.-Y. (2017). Visualizing Chemical Functionality in Plant Cell Walls. Biotechnol. Biofuels, 10.","DOI":"10.1186\/s13068-017-0953-3"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1007\/s00216-015-9290-1","article-title":"Analysis of Saccharides in Beverages by HPLC with Direct UV Detection","volume":"408","author":"Schmid","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yang, J., Zhang, T., Tian, C., Zhu, Y., Zeng, Y., Men, Y., Chen, P., Sun, Y., and Ma, Y. (2019). Multi-Enzyme Systems and Recombinant Cells for Synthesis of Valuable Saccharides: Advances and Perspectives. Biotechnol. Adv., 37.","DOI":"10.1016\/j.biotechadv.2019.06.005"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"339226","DOI":"10.1016\/j.aca.2021.339226","article-title":"Glucose detection through surface-enhanced Raman spectroscopy: A review","volume":"1206","author":"Sun","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1108\/SR-01-2019-0017","article-title":"Glucose oxidase-based biosensor for glucose detection from biological fluids","volume":"40","author":"Mandpe","year":"2020","journal-title":"Sens. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Benalaya, I., Alves, G., Lopes, J., and Silva, L.R. (2024). A Review of Natural Polysaccharides: Sources, Characteristics, Properties, Food, and Pharmaceutical Applications. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25021322"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"13868","DOI":"10.1038\/ncomms13868","article-title":"Electronic Single-Molecule Identification of Carbohydrate Isomers by Recognition Tunnelling","volume":"7","author":"Im","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1080\/01635581.2021.1895233","article-title":"An Investigation into the Usage of Monosaccharides with GLUT1 and GLUT3 as Prognostic Indicators for Cancer","volume":"74","year":"2022","journal-title":"Nutr. Cancer"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Yahia, E.M., Carrillo-L\u00f3pez, A., and Bello-Perez, L.A. (2019). Carbohydrates. Postharvest Physiology and Biochemistry of Fruits and Vegetables, Elsevier.","DOI":"10.1016\/B978-0-12-813278-4.00009-9"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7867","DOI":"10.1021\/acs.chemrev.7b00669","article-title":"Capillary Electrophoresis Separations of Glycans","volume":"118","author":"Lu","year":"2018","journal-title":"Chem. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"118218","DOI":"10.1016\/j.carbpol.2021.118218","article-title":"A General Strategy for the Structural Determination of Carbohydrates by Multi-Dimensional NMR Spectroscopies","volume":"267","author":"Shi","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"118885","DOI":"10.1016\/j.carbpol.2021.118885","article-title":"Liquid-State NMR Spectroscopy for Complex Carbohydrate Structural Analysis: A Hitchhiker\u2019s Guide","volume":"277","author":"Speciale","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.sbi.2018.04.003","article-title":"Using Structurally Defined Oligosaccharides to Understand the Interactions between Proteins and Heparan Sulfate","volume":"50","author":"Xu","year":"2018","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bhuniya, S., Demina, T.S., and Akopova, T.A. (2024). Advances in Applications of Polysaccharides and Polysaccharide-Based Materials. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25126482"},{"key":"ref_17","first-page":"20160053","article-title":"Novel Nanoparticle Materials for Drug\/Food Delivery-Polysaccharides","volume":"1","author":"Chen","year":"2016","journal-title":"Phys. Sci. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1021\/acs.jafc.3c06433","article-title":"Structurally Modified Polysaccharides: Physicochemical Properties, Biological Activities, Structure\u2013Activity Relationship, and Applications","volume":"72","author":"Chen","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"139408","DOI":"10.1016\/j.foodchem.2024.139408","article-title":"Polysaccharides in Fruits: Biological Activities, Structures, and Structure-Activity Relationships and Influencing Factors-A Review","volume":"451","author":"Shi","year":"2024","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.ijbiomac.2010.03.026","article-title":"Study on Molecular Structural Characteristics of Tea Polysaccharide","volume":"47","author":"Guo","year":"2010","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8260","DOI":"10.1021\/acs.energyfuels.6b01390","article-title":"Improvement of Acid Hydrolysis Procedures for the Composition Analysis of Herbaceous Biomass","volume":"30","author":"Whitfield","year":"2016","journal-title":"Energy Fuels"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.lwt.2016.01.066","article-title":"Analysis of Molecular Structure of Starch Citrate Obtained by a Well-Stablished Method","volume":"69","author":"Buksa","year":"2016","journal-title":"LWT Food Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41477-018-0330-7","article-title":"The Molecular Structure of Plant Sporopollenin","volume":"5","author":"Li","year":"2018","journal-title":"Nat. Plants"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"110290","DOI":"10.1016\/j.foodres.2021.110290","article-title":"A Review of NMR Analysis in Polysaccharide Structure and Conformation: Progress, Challenge and Perspective","volume":"143","author":"Yao","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"113201","DOI":"10.1016\/j.lwt.2022.113201","article-title":"Structure-Activity Relationship of Antioxidant Polysaccharides from Huangshui Based on the HPLC Fingerprint Combined with Chemometrics Methods","volume":"159","author":"Huo","year":"2022","journal-title":"LWT"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"14463","DOI":"10.1021\/jacs.9b06406","article-title":"Advancing Solutions to the Carbohydrate Sequencing Challenge","volume":"141","author":"Gray","year":"2019","journal-title":"J. Am. Chem. Soc."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"BeMiller, J.N. (2019). Polysaccharides. Carbohydrate Chemistry for Food Scientists, Elsevier.","DOI":"10.1016\/B978-0-12-812069-9.05001-9"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1126\/science.adh3856","article-title":"Direct Observation of Glycans Bonded to Proteins and Lipids at the Single-Molecule Level","volume":"382","author":"Anggara","year":"2023","journal-title":"Science"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/s41586-020-2362-1","article-title":"Imaging Single Glycans","volume":"582","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"143678","DOI":"10.1016\/j.electacta.2023.143678","article-title":"Nanopore Electrochemical Sensors for Emerging Hazardous Pollutants Detection","volume":"475","author":"Ma","year":"2024","journal-title":"Electrochim. Acta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13770","DOI":"10.1073\/pnas.93.24.13770","article-title":"Characterization of Individual Polynucleotide Molecules Using a Membrane Channel","volume":"93","author":"Kasianowicz","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/35093038","article-title":"Stochastic Sensors Inspired by Biology","volume":"413","author":"Bayley","year":"2001","journal-title":"Nature"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1093\/nsr\/nwy029","article-title":"A Single Biomolecule Interface for Advancing the Sensitivity, Selectivity and Accuracy of Sensors","volume":"5","author":"Ying","year":"2018","journal-title":"Natl. Sci. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/S0958-1669(99)80017-2","article-title":"Designed Membrane Channels and Pores","volume":"10","author":"Bayley","year":"1999","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/35084037","article-title":"Ion-Beam Sculpting at Nanometre Length Scales","volume":"412","author":"Li","year":"2001","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/nmat941","article-title":"Fabrication of Solid-State Nanopores with Single-Nanometre Precision","volume":"2","author":"Storm","year":"2003","journal-title":"Nat. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6368","DOI":"10.1021\/acsabm.0c00812","article-title":"Nanopore Fabrication via Transient High Electric Field Controlled Breakdown and Detection of Single RNA Molecules","volume":"3","author":"Yin","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12197","DOI":"10.1021\/acs.jpcc.2c02674","article-title":"Sensing the Performance of Artificially Intelligent Nanopores Developed by Integrating Solid-State Nanopores with Machine Learning Methods","volume":"126","author":"Taniguchi","year":"2022","journal-title":"J. Phys. Chem. C"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2205680","DOI":"10.1002\/smll.202205680","article-title":"Solid-State Nanopore Array: Manufacturing and Applications","volume":"19","author":"Liu","year":"2023","journal-title":"Small"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1039\/D3AY02040A","article-title":"Recent Advances in Nanopore-Based Analysis for Carbohydrates and Glycoconjugates","volume":"16","author":"Zhao","year":"2024","journal-title":"Anal. Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6229","DOI":"10.1039\/D4SC01466A","article-title":"Nanopore-Based Glycan Sequencing: State of the Art and Future Prospects","volume":"15","author":"Yao","year":"2024","journal-title":"Chem. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1021\/acs.biochem.6b00349","article-title":"Kinetic Basis of Carbohydrate-Mediated Inhibition of Human Glucokinase by the Glucokinase Regulatory Protein","volume":"55","author":"Casey","year":"2016","journal-title":"Biochemistry"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s11684-022-0955-9","article-title":"Decreasing Complexity of Glucose Time Series Derived from Continuous Glucose Monitoring Is Correlated with Deteriorating Glucose Regulation","volume":"17","author":"Li","year":"2023","journal-title":"Front. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1002\/bit.10830","article-title":"Genetically Engineered Binding Proteins as Biosensors for Fermentation and Cell Culture","volume":"84","author":"Ge","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1016\/j.febslet.2010.04.043","article-title":"A Structural Classification of Substrate-binding Proteins","volume":"584","author":"Berntsson","year":"2010","journal-title":"FEBS Lett."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1038\/s41467-018-06534-1","article-title":"Direct Electrical Quantification of Glucose and Asparagine from Bodily Fluids Using Nanopores","volume":"9","author":"Galenkamp","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"14303","DOI":"10.1021\/acs.analchem.0c03399","article-title":"Probing the Neuraminidase Activity of Influenza Virus Using a Cytolysin A Protein Nanopore","volume":"92","author":"Kwak","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8032","DOI":"10.1039\/c3cs60148j","article-title":"Selective Sensing of Saccharides Using Simple Boronic Acids and Their Aggregates","volume":"42","author":"Wu","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.1002\/ange.201712740","article-title":"Single-Molecule Determination of the Isomers of d -Glucose and d -Fructose That Bind to Boronic Acids","volume":"130","author":"Ramsay","year":"2018","journal-title":"Angew. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8001","DOI":"10.1021\/cr500562m","article-title":"Glucose Sensing in Supramolecular Chemistry","volume":"115","author":"Sun","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e202203769","DOI":"10.1002\/anie.202203769","article-title":"A Nanopore-Based Saccharide Sensor","volume":"61","author":"Zhang","year":"2022","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"215816","DOI":"10.1016\/j.ccr.2024.215816","article-title":"Solid-State Nanopore DNA Sequencing: Advances, Challenges and Prospects","volume":"510","author":"He","year":"2024","journal-title":"Coord. Chem. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Liu, L., Ma, X., Chang, Y., Guo, H., and Wang, W. (2023). Biosensors with Boronic Acid-Based Materials as the Recognition Elements and Signal Labels. Biosensors, 13.","DOI":"10.3390\/bios13080785"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/161096b0","article-title":"Reaction of boric acid with polysaccharides","volume":"161","author":"Deuel","year":"1948","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1021\/acssensors.1c00462","article-title":"Molecular Boronic Acid-Based Saccharide Sensors","volume":"6","author":"Williams","year":"2021","journal-title":"ACS Sens."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.1039\/c2cc17277a","article-title":"pH Gated Glucose Responsive Biomimetic Single Nanochannels","volume":"48","author":"Sun","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.elecom.2013.09.009","article-title":"Sugar-Stimulated Robust Nanodevice: 4-Carboxyphenylboronic Acid Modified Single Glass Conical Nanopores","volume":"36","author":"Zhao","year":"2013","journal-title":"Electrochem. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"11205","DOI":"10.1016\/j.tet.2004.08.051","article-title":"The Relationship among pKa, pH, and Binding Constants in the Interactions between Boronic Acids and Diols\u2014It Is Not as Simple as It Appears","volume":"60","author":"Yan","year":"2004","journal-title":"Tetrahedron"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1039\/C6NR07339E","article-title":"A Temperature, pH and Sugar Triple-Stimuli-Responsive Nanofluidic Diode","volume":"9","author":"Zheng","year":"2017","journal-title":"Nanoscale"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"9214","DOI":"10.1039\/c3nr02105j","article-title":"Carbohydrate-Actuated Nanofluidic Diode: Switchable Current Rectification in a Nanopipette","volume":"5","author":"Vilozny","year":"2013","journal-title":"Nanoscale"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"14029","DOI":"10.1021\/acs.analchem.9b03646","article-title":"Rational Design of Stimuli-Responsive Polymers Modified Nanopores for Selective and Sensitive Determination of Salivary Glucose","volume":"91","author":"Yang","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/S0006-3495(02)75442-8","article-title":"Transport of Maltodextrins through Maltoporin: A Single-Channel Study","volume":"82","author":"Kullman","year":"2002","journal-title":"Biophys. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1021\/acsnano.9b07385","article-title":"Different Anomeric Sugar Bound States of Maltose Binding Protein Resolved by a Cytolysin A Nanopore Tweezer","volume":"14","author":"Li","year":"2020","journal-title":"ACS Nano"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.bbrc.2011.07.121","article-title":"Discrimination of Neutral Oligosaccharides through a Nanopore","volume":"412","author":"Bacri","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1038\/s41467-023-37348-5","article-title":"Identification of Tagged Glycans with a Protein Nanopore","volume":"14","author":"Li","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1021\/acssensors.2c00943","article-title":"Selective Translocation of Cyclic Sugars through Dynamic Bacterial Transporter","volume":"7","author":"Vikraman","year":"2022","journal-title":"ACS Sens."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"18812","DOI":"10.1021\/jacs.3c03563","article-title":"Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered \u03b1-Hemolysin Nanopores","volume":"145","author":"Xia","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"13356","DOI":"10.1021\/jacs.4c02081","article-title":"Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore","volume":"146","author":"Yao","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"9672","DOI":"10.1021\/nn3031047","article-title":"Single Molecule Detection of Glycosaminoglycan Hyaluronic Acid Oligosaccharides and Depolymerization Enzyme Activity Using a Protein Nanopore","volume":"6","author":"Fennouri","year":"2012","journal-title":"ACS Nano"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1140\/epje\/i2018-11733-5","article-title":"Comparative biosensing of glycosaminoglycan hyaluronic acid oligo-and polysaccharides using aerolysin and \u03b1-hemolysin nanopores","volume":"41","author":"Fennouri","year":"2018","journal-title":"Eur. Phys. J. E"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"48003","DOI":"10.1209\/0295-5075\/82\/48003","article-title":"Effect of Screening on the Transport of Polyelectrolytes through Nanopores","volume":"82","author":"Oukhaled","year":"2008","journal-title":"Europhys. Lett."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"5639","DOI":"10.1021\/acs.nanolett.4c01223","article-title":"Single-Molecule Discrimination of Saccharides Using Carbon Nitride Nanopores","volume":"24","author":"Guo","year":"2024","journal-title":"Nano Lett."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/nmat2317","article-title":"A Metal-Free Polymeric Photocatalyst for Hydrogen Production from Water under Visible Light","volume":"8","author":"Wang","year":"2009","journal-title":"Nat. Mater."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Qiu, M., Huang, S., Luo, C., Wu, Z., Liang, B., Huang, H., Ci, Z., Zhang, D., Han, L., and Lin, J. (2021). Pharmacological and Clinical Application of Heparin Progress: An Essential Drug for Modern Medicine. Biomed. Pharmacother., 139.","DOI":"10.1016\/j.biopha.2021.111561"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"3278","DOI":"10.1038\/s41467-018-05751-y","article-title":"Surveying Silicon Nitride Nanopores for Glycomics and Heparin Quality Assurance","volume":"9","author":"Karawdeniya","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"6308","DOI":"10.1021\/acsnano.9b00618","article-title":"Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores","volume":"13","author":"Im","year":"2019","journal-title":"ACS Nano"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1089\/dna.2004.23.675","article-title":"Nanopore Cheminformatics","volume":"23","author":"Akeson","year":"2004","journal-title":"DNA Cell Biol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e2022806118","DOI":"10.1073\/pnas.2022806118","article-title":"Synthetic Heparan Sulfate Standards and Machine Learning Facilitate the Development of Solid-State Nanopore Analysis","volume":"118","author":"Xia","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.bios.2019.05.005","article-title":"Single Conical Track-Etched Nanopore for a Free-Label Detection of OSCS Contaminants in Heparin","volume":"137","author":"Ma","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1046\/j.1365-2796.1997.00170.x","article-title":"Hyaluronan: Its Nature, Distribution, Functions and Turnover","volume":"242","author":"Fraser","year":"1997","journal-title":"J. Intern. Med."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Cowman, M.K., Lee, H.-G., Schwertfeger, K.L., McCarthy, J.B., and Turley, E.A. (2015). The Content and Size of Hyaluronan in Biological Fluids and Tissues. Front. Immunol., 6.","DOI":"10.3389\/fimmu.2015.00261"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"8488","DOI":"10.1021\/ac4020929","article-title":"Kinetics of enzymatic degradation of high molecular weight polysaccharides through a nanopore: Experiments and data-modeling","volume":"85","author":"Fennouri","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"5113","DOI":"10.1038\/s41467-022-32800-4","article-title":"Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore","volume":"13","author":"Bayat","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1038\/s41467-018-03439-x","article-title":"Label-Free Analysis of Physiological Hyaluronan Size Distribution with a Solid-State Nanopore Sensor","volume":"9","author":"Rivas","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"7231","DOI":"10.1039\/D0NR00645A","article-title":"Dynamics of Long Hyaluronic Acid Chains through Conical Nanochannels for Characterizing Enzyme Reactions in Confined Spaces","volume":"12","author":"Ma","year":"2020","journal-title":"Nanoscale"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.tplants.2004.02.005","article-title":"The Role of Plant Cell Wall Polysaccharide Composition in Disease Resistance","volume":"9","author":"Vorwerk","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"R865","DOI":"10.1016\/j.cub.2017.05.025","article-title":"Plant Cell Walls","volume":"27","author":"Voxeur","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/jipb.13055","article-title":"The Plant Cell Wall: Biosynthesis, Construction, and Functions","volume":"63","author":"Zhang","year":"2021","journal-title":"J. Integr. Plant Biol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"100106","DOI":"10.1016\/j.xplc.2020.100106","article-title":"A Solid-State Nanopore-Based Single-Molecule Approach for Label-Free Characterization of Plant Polysaccharides","volume":"2","author":"Cai","year":"2021","journal-title":"Plant Commun."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1002\/cjoc.202300031","article-title":"Application of Nanopore Single Molecule Detection Technology in Analysis of Xylan Dissolved in Ionic Liquid","volume":"41","author":"Xie","year":"2023","journal-title":"Chin. J. Chem."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Xie, W., He, S., Fang, S., Yin, B., Tian, R., Wang, Y., and Wang, D. (2023). Analysis of Starch Dissolved in Ionic Liquid by Glass Nanopore at Single Molecular Level. Int. J. Biol. Macromol., 239.","DOI":"10.1016\/j.ijbiomac.2023.124271"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"7147","DOI":"10.1039\/D2NR07280G","article-title":"Phenylboronic Acid-Modified Polyethyleneimine Assisted Neutral Polysaccharide Detection and Weight-Resolution Analysis with a Nanopipette","volume":"15","author":"Xie","year":"2023","journal-title":"Nanoscale"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1001\/archsurg.1967.01330110080011","article-title":"Dextran 40 and Dextran 70: A Review","volume":"94","author":"Atik","year":"1967","journal-title":"Arch. Surg."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1177\/0310057X1704500506","article-title":"In Vitro Evaluation of the Effect of Haemodilution with Dextran 40 on Coagulation Profile as Measured by Thromboelastometry and Multiple Electrode Aggregometry","volume":"45","author":"Kam","year":"2017","journal-title":"Anaesth. Intensive Care"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/nature05816","article-title":"Glycan-Based Interactions Involving Vertebrate Sialic-Acid-Recognizing Proteins","volume":"446","author":"Varki","year":"2007","journal-title":"Nature"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1002\/ange.201710984","article-title":"Quantitative Screening of Cell-Surface Gangliosides by Nondestructive Extraction and Hydrophobic Collection","volume":"130","author":"Chen","year":"2018","journal-title":"Angew. Chem."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1039\/C9SC05319K","article-title":"Biomimetic Nanochannels for the Discrimination of Sialylated Glycans via a Tug-of-War between Glycan Binding and Polymer Shrinkage","volume":"11","author":"Li","year":"2020","journal-title":"Chem. Sci."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"(2005). Session 27: Post-Translational Modifications-Glycosylation and Glycoproteomics. Mol. Cell. Proteom., 4, S262\u2013S267.","DOI":"10.1016\/S1535-9476(20)34762-9"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Illiano, A., Pinto, G., Melchiorre, C., Carpentieri, A., Faraco, V., and Amoresano, A. (2020). Protein Glycosylation Investigated by Mass Spectrometry: An Overview. Cells, 9.","DOI":"10.3390\/cells9091986"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Khoury, G.A., Baliban, R.C., and Floudas, C.A. (2011). Proteome-Wide Post-Translational Modification Statistics: Frequency Analysis and Curation of the Swiss-Prot Database. Sci. Rep., 1.","DOI":"10.1038\/srep00090"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"10530","DOI":"10.1021\/acs.chemrev.5b00321","article-title":"Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence","volume":"115","author":"Chinen","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"7957","DOI":"10.1021\/acs.nanolett.9b03134","article-title":"Label-Free Detection of Post-Translational Modifications with a Nanopore","volume":"19","author":"Wong","year":"2019","journal-title":"Nano Lett."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"5357","DOI":"10.1021\/acs.nanolett.2c01338","article-title":"Quantification of Protein Glycosylation Using Nanopores","volume":"22","author":"Versloot","year":"2022","journal-title":"Nano Lett."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"5715","DOI":"10.1021\/acs.analchem.2c00860","article-title":"Nanopore-Based Single-Entity Electrochemistry for the Label-Free Monitoring of Single-Molecule Glycoprotein\u2013Boronate Affinity Interaction and Its Sensing Application","volume":"94","author":"Tang","year":"2022","journal-title":"Anal. Chem."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.snb.2011.12.070","article-title":"Saccharide\/Glycoprotein Recognition inside Synthetic Ion Channels Modified with Boronic Acid","volume":"162","author":"Nguyen","year":"2012","journal-title":"Sens. Actuators B Chem."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nrmicro.2016.25","article-title":"Lipopolysaccharide Transport and Assembly at the Outer Membrane: The PEZ Model","volume":"14","author":"Okuda","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Di Guida, R., Casillo, A., Tom\u00e1s, J.M., Merino, S., and Corsaro, M.M. (2022). Complete Characterization of the O-Antigen from the LPS of Aeromonas bivalvium. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031204"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"15975","DOI":"10.1021\/jacs.1c04910","article-title":"Synthetic Macrocycle Nanopore for Potassium-Selective Transmembrane Transport","volume":"143","author":"Qiao","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1007\/s12274-016-1041-x","article-title":"Peptide Recognition by Functional Supramolecular Nanopores with Complementary Size and Binding Sites","volume":"9","author":"Chen","year":"2016","journal-title":"Nano Res."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1021\/acs.nanolett.1c04633","article-title":"Single Molecule DNA Analysis Based on Atomic-Controllable Nanopores in Covalent Organic Frameworks","volume":"22","author":"Xing","year":"2022","journal-title":"Nano Lett."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"9851","DOI":"10.1021\/acs.analchem.2c01708","article-title":"High Spatial Resolution of Ultrathin Covalent Organic Framework Nanopores for Single-Molecule DNA Sensing","volume":"94","author":"Xing","year":"2022","journal-title":"Anal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/16\/5442\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:41:37Z","timestamp":1760110897000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/16\/5442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,22]]},"references-count":111,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["s24165442"],"URL":"https:\/\/doi.org\/10.3390\/s24165442","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,22]]}}}