{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T06:40:10Z","timestamp":1781851210439,"version":"3.54.5"},"reference-count":23,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T00:00:00Z","timestamp":1726617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council","award":["112-2314-B-038-063"],"award-info":[{"award-number":["112-2314-B-038-063"]}]},{"name":"National Science and Technology Council","award":["113-2314-B-038-121"],"award-info":[{"award-number":["113-2314-B-038-121"]}]},{"name":"Ministry of Education","award":["112-2314-B-038-063"],"award-info":[{"award-number":["112-2314-B-038-063"]}]},{"name":"Ministry of Education","award":["113-2314-B-038-121"],"award-info":[{"award-number":["113-2314-B-038-121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Long-term observation of single-cell oscillations within tissue networks is now possible by combining bioluminescence reporters with stable tissue explant culture techniques. This method is particularly effective in revealing the network dynamics in systems with slow oscillations, such as circadian clocks. However, the low intensity of luciferase-based bioluminescence requires signal amplification using specialized cameras (e.g., I-CCDs and EM-CCDs) and prolonged exposure times, increasing baseline noise and reducing temporal resolution. To address this limitation, we implemented a cost-effective optical enhancement technique called telecompression, first used in astrophotography and now commonly used in digital photography. By combining a high numerical aperture objective lens with a magnification-reducing relay lens, we significantly increased the collection efficiency of the bioluminescence signal without raising the baseline CCD noise. This method allows for shorter exposure times in time-lapse imaging, enhancing temporal resolution and enabling more precise period estimations. Our implementation demonstrates the feasibility of telecompression for enhancing bioluminescence imaging for the tissue-level network observation of circadian clocks.<\/jats:p>","DOI":"10.3390\/s24186041","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T04:59:54Z","timestamp":1726721994000},"page":"6041","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhancing Bioluminescence Imaging of Cultured Tissue Explants Using Optical Telecompression"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2529-8013","authenticated-orcid":false,"given":"Jihwan","family":"Myung","sequence":"first","affiliation":[{"name":"Braintime Laboratory, Graduate Institute of Mind, Brain and Consciousness (GIMBC), Taipei Medical University, New Taipei City 235, Taiwan"},{"name":"Graduate Institute of Medical Sciences, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E3911","DOI":"10.1073\/pnas.1420753112","article-title":"Distinct roles for GABA across multiple timescales in mammalian circadian timekeeping","volume":"112","author":"DeWoskin","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0165-0270(91)90128-M","article-title":"A simple method for organotypic cultures of nervous tissue","volume":"37","author":"Stoppini","year":"1991","journal-title":"J. Neurosci. Methods"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"E3920","DOI":"10.1073\/pnas.1421200112","article-title":"GABA-mediated repulsive coupling between circadian clock neurons in the SCN encodes seasonal time","volume":"112","author":"Myung","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1021\/cr078213v","article-title":"Genetically encoded calcium indicators","volume":"108","author":"Mank","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0003-2697(90)90601-5","article-title":"Reporter genes: Application to the study of mammalian gene transcription","volume":"188","author":"Alam","year":"1990","journal-title":"Anal. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev.bb.18.060189.001415","article-title":"Luminescence digital imaging microscopy","volume":"18","author":"Jovin","year":"1989","journal-title":"Annu. Rev. Biophys. Biophys. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1021\/acsmeasuresciau.3c00036","article-title":"Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power","volume":"3","author":"Klein","year":"2023","journal-title":"ACS Meas. Sci. Au"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/j.1751-1097.1997.tb03184.x","article-title":"Visualizing gene expression in living mammals using a bioluminescent reporter","volume":"66","author":"Contag","year":"1997","journal-title":"Photochem. Photobiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1126\/science.1089287","article-title":"Synchronization of cellular clocks in the suprachiasmatic nucleus","volume":"302","author":"Yamaguchi","year":"2003","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7664","DOI":"10.1073\/pnas.0607713104","article-title":"Separate oscillating cell groups in mouse suprachiasmatic nucleus couple photoperiodically to the onset and end of daily activity","volume":"104","author":"Inagaki","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Nakajima, Y., Yamazaki, T., Nishii, S., Noguchi, T., Hoshino, H., Niwa, K., Viviani, V.R., and Ohmiya, Y. (2010). Enhanced beetle luciferase for high-resolution bioluminescence imaging. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010011"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8900","DOI":"10.1523\/JNEUROSCI.5586-11.2012","article-title":"Period coding of Bmal1 oscillators in the suprachiasmatic nucleus","volume":"32","author":"Myung","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0076-6879(05)93011-5","article-title":"Real-time reporting of circadian-regulated gene expression by luciferase imaging in plants and mammalian cells","volume":"393","author":"Welsh","year":"2005","journal-title":"Methods Enzymol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Garfinkle, R.A., and Garfinkle, R.A. (2020). Photographing the Moon. Luna Cognita: A Comprehensive Observer\u2019s Handbook of the Known Moon, Springer.","DOI":"10.1007\/978-1-4939-1664-1"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Brady, D.J. (2009). Optical Imaging and Spectroscopy, John Wiley & Sons.","DOI":"10.1002\/9780470443736"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Christenson, M.A. (2002). Detection systems optimized for low-light chemiluminescence imaging. Luminescence Biotechnology: Instruments and Applications, CRC Press.","DOI":"10.1201\/9781420041804.ch36"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.chembiol.2020.07.022","article-title":"Seeing (and using) the light: Recent developments in bioluminescence technology","volume":"27","author":"Love","year":"2020","journal-title":"Cell Chem. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.micron.2017.11.006","article-title":"Oblique illumination in microscopy: A quantitative evaluation","volume":"105","author":"Sanchez","year":"2018","journal-title":"Micron"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5339","DOI":"10.1073\/pnas.0308709101","article-title":"PERIOD2:: LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues","volume":"101","author":"Yoo","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Morales-Curiel, L.F., Gonzalez, A.C., Castro-Olvera, G., Lin, L.C., El-Quessny, M., Porta-de-la-Riva, M., Severino, J., Morera, L.B., Venturini, V., and Ruprecht, V. (2022). Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy. Commun. Biol., 5.","DOI":"10.1038\/s42003-022-04292-x"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Saito-Moriya, R., Nakayama, J., Kamiya, G., Kitada, N., Obata, R., Maki, S.A., and Aoyama, H. (2021). How to select firefly luciferin analogues for in vivo imaging. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22041848"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"eadm6869","DOI":"10.1126\/science.adm6869","article-title":"Achieving optical transparency in live animals with absorbing molecules","volume":"385","author":"Ou","year":"2024","journal-title":"Science"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1051\/0004-6361:20053695","article-title":"Lucky imaging: High angular resolution imaging in the visible from the ground","volume":"446","author":"Law","year":"2006","journal-title":"Astron. Astrophys."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/18\/6041\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:58:53Z","timestamp":1760111933000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/24\/18\/6041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,18]]},"references-count":23,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["s24186041"],"URL":"https:\/\/doi.org\/10.3390\/s24186041","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,18]]}}}