{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T21:01:07Z","timestamp":1775250067538,"version":"3.50.1"},"reference-count":16,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T00:00:00Z","timestamp":1238371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat treatment. Streptavidin (STA) and biotinylated bovine serum albumin (Bio-BSA) were chosen as the model receptor and the model analyte, respectively, to demonstrate the effectiveness of this detection method. Using this model system, we were able to enhance the sensitivity in monitoring the binding of Bio-BSA to gold nano-island surfaces functionalized with STA through the addition of gold nanoparticle-STA conjugates. In addition, SU-8 well chips with gold nano-island surfaces were fabricated through a conventional UV patterning method and were then utilized for image detection using the attenuated total reflection mode. These results suggest that the gold nano-island well chip may have the potential to be used for multiple and simultaneous detection of various bio-substances.<\/jats:p>","DOI":"10.3390\/s90402334","type":"journal-article","created":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T05:00:22Z","timestamp":1238475622000},"page":"2334-2344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles"],"prefix":"10.3390","volume":"9","author":[{"given":"Hyung Min","family":"Kim","sequence":"first","affiliation":[{"name":"Fusion Biotechnology Research Center, KRICT, Daejeon 305-600, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Min","family":"Jin","sequence":"additional","affiliation":[{"name":"Fusion Biotechnology Research Center, KRICT, Daejeon 305-600, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok Kee","family":"Lee","sequence":"additional","affiliation":[{"name":"BioMonitoring Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong-gu, Daejeon, 305\u2013333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Gon","family":"Kim","sequence":"additional","affiliation":[{"name":"BioMonitoring Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong-gu, Daejeon, 305\u2013333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Beom","family":"Shin","sequence":"additional","affiliation":[{"name":"BioMonitoring Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong-gu, Daejeon, 305\u2013333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2009,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/BF01395428","article-title":"Die bestimmung optischer konstanten von metallen durch anregung von oberfl\u00e4chenplasmaschwingungen","volume":"241","author":"Kretschmann","year":"1971","journal-title":"Z. Phys. A"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4554","DOI":"10.1021\/ac990639p","article-title":"In situ monitoring of the change in extinction of stabilized nanoscopic gold particles in contact with aqueous phenol solutions","volume":"71","author":"Gluodenis","year":"1999","journal-title":"Anal. Chem"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1021\/ja003312a","article-title":"Chain length dependence and sensing capabilities of the localized surface plasmon resonance of silver nanoparticles chemically modified with alkanethiol self-assembled monolayers","volume":"123","author":"Malinsky","year":"2001","journal-title":"J. Am. Chem. Soc"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1021\/ac015657x","article-title":"A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on surface","volume":"74","author":"Nath","year":"2002","journal-title":"Anal. Chem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6894","DOI":"10.1021\/ac0346609","article-title":"Biosensing based on light absorption of nanoscaled gold and silver particles","volume":"75","author":"Frederix","year":"2003","journal-title":"Anal. Chem"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7365","DOI":"10.1021\/la0489054","article-title":"Biological sensing using transmission surface plasmon resonance spectroscopy","volume":"20","author":"Lahav","year":"2004","journal-title":"Langmuir"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1016\/j.bios.2006.12.028","article-title":"Analysis of recombinant protein expression using localized surface plasmon resonance (LSPR)","volume":"22","author":"Shin","year":"2007","journal-title":"Biosens. Bioelectron"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1021\/ac800087u","article-title":"Label-free detection of melittin binding to a membrane using electrochemical localized surface plasmon resonance","volume":"80","author":"Hiep","year":"2008","journal-title":"Anal. Chem"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3476","DOI":"10.1021\/cm049605a","article-title":"Ultrathin gold island films on silanized glass. Morphology and optical properties","volume":"16","author":"Barkay","year":"2004","journal-title":"Chem. Mater"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3849","DOI":"10.1002\/1521-3765(20020902)8:17<3849::AID-CHEM3849>3.0.CO;2-1","article-title":"Transmission surface plasmon resonance (T-SPR) measurements for monitoring adsorption on ultrathin gold island films","volume":"8","author":"Kalyuzhny","year":"2002","journal-title":"Chem. Eur. J"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.1021\/ac049741z","article-title":"Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size","volume":"76","author":"Nath","year":"2004","journal-title":"Anal. Chem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7337","DOI":"10.1021\/jp034234r","article-title":"Nanoparticle Optics: The importance of radiative dipole coupling in two-dimensional nanoparticle arrays","volume":"107","author":"Haynes","year":"2003","journal-title":"J. Phys. Chem. B"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Raether, H. (1988). Surface plasmons on smooth and rough surfaces and on gratings, Springer-Verlag.","DOI":"10.1007\/BFb0048317"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0925-4005(98)00321-9","article-title":"Surface plasmon resonance sensors: review","volume":"54","author":"Homola","year":"1999","journal-title":"Sens. Actuat. B"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1021\/jp027562k","article-title":"Optical properties of short range ordered arrays of nanometer gold disks prepared by colloidal lithography","volume":"107","author":"Hanarp","year":"2003","journal-title":"J. Phys. Chem. B"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.talanta.2005.06.039","article-title":"Toward advanced chemical and biological nanosensors-An overview","volume":"67","author":"Yonzon","year":"2005","journal-title":"Talanta"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/9\/4\/2334\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:10:08Z","timestamp":1760220608000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/9\/4\/2334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,30]]},"references-count":16,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,4]]}},"alternative-id":["s90402334"],"URL":"https:\/\/doi.org\/10.3390\/s90402334","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,30]]}}}