{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T04:31:02Z","timestamp":1776141062914,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T00:00:00Z","timestamp":1253664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal alternative for studies that require long-term imaging of cells such as detection of low abundance cellular antigens by fluorescence microscopy. The advantage of Qdot labeled probes over conventional immunological methods is the photostability of the probe. Phycoerythrin bleaches in cyanobacterial cells under prolonged UV or blue light excitation, which means that the semiconducting nanocrystal probe, the Qdot, can yield a strong fluorescent signal without interference from cellular pigments.<\/jats:p>","DOI":"10.3390\/s90907540","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T11:03:56Z","timestamp":1253703836000},"page":"7540-7549","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates"],"prefix":"10.3390","volume":"9","author":[{"given":"Karen M.","family":"Orcutt","sequence":"first","affiliation":[{"name":"Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd., Stennis Space Center, MS 39529, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd., Stennis Space Center, MS 39529, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kjell","family":"Gundersen","sequence":"additional","affiliation":[{"name":"Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd., Stennis Space Center, MS 39529, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2009,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3354\/ame01197","article-title":"Whole-cell immunocytochemical detection of nitrogenase in cyanobacteria: improved protocol for highly fluorescent cells","volume":"51","author":"Taniuchi","year":"2008","journal-title":"Aquat. 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Microbiol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/35088063","article-title":"Unicellular cyanobacteria fix N2 in the subropical North Pacific Ocean","volume":"412","author":"Zehr","year":"2001","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/nature02824","article-title":"High rates of N2 fixation by unicellular diazotrophs in the oliggotrophic Pacific Ocean","volume":"430","author":"Montoya","year":"2004","journal-title":"Nature"},{"key":"ref_12","first-page":"71","article-title":"Biological and ecological characterization of the marine cyanobacterium Synechococcus","volume":"214","author":"Waterbury","year":"1986","journal-title":"Can. Bull. Fish. Aquat. Sci"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1111\/j.0022-3646.1996.00916.x","article-title":"Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium spp. IMS101 in defined media: Evidence of a circadium rhythm","volume":"32","author":"Chen","year":"1996","journal-title":"J. Phycol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4319\/lo.1990.35.1.0059","article-title":"Immunofluorescence detection and characterization of N2-fixing microorganisms from aquatic environments","volume":"35","author":"Currin","year":"1990","journal-title":"Limnol. Oceanogr"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3354\/ame01185","article-title":"High abundance of diazotrophic picocyanobacteria (<3\u03bcm) in a Southwest Pacific coral lagoon","volume":"51","author":"Biegala","year":"2008","journal-title":"Aquat. Microb. 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