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Dalian","award":["GML2019ZD0402"],"award-info":[{"award-number":["GML2019ZD0402"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["31902395"],"award-info":[{"award-number":["31902395"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["2018YFD0901604"],"award-info":[{"award-number":["2018YFD0901604"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["2020RQ115"],"award-info":[{"award-number":["2020RQ115"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["XLYC2002107"],"award-info":[{"award-number":["XLYC2002107"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["2020RD03"],"award-info":[{"award-number":["2020RD03"]}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)","award":["GML2019ZD0402"],"award-info":[{"award-number":["GML2019ZD0402"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Symmetry"],"abstract":"<jats:p>Animals are not only regulated by their own genes but also influenced by symbiotic bacteria, most of which are colonized in the gut. The gut bacterial community is involved in plenty of physiological processes; therefore, intestinal colonization by commensal microbiota is essential to the health of the host animal. Here, metagenome sequencing of the A. japonicus gut, surrounding water, and feed was performed to explore the structural and functional characteristics of the colonized bacteria in the gut of A. japonicus. Results showed that Bacteroidetes and Proteobacteria were the main dominant phyla of the A. japonicus gut, and Formosa, Vibrio, and Lactobacillus were the dominant genera. There was asymmetry between the A. japonicus gut and its surrounding environment in the bacterial community. In terms of the top 50 abundant genera, those colonized in the gut shared a similarity of 26% with those colonized in the surrounding water and a similarity of 30% with those colonized in the feed. According to KEGG annotation, the dominant metabolic pathways in the gut of A. japonicus were glycan biosynthesis and metabolism, nitrogen metabolism, and cysteine and methionine metabolism. This implies that the gut-colonized bacteria of A. japonicus are influenced by the surrounding water and the feed. In addition, the gut-colonized bacteria might be related to the growth and metabolism of A. japonicus.<\/jats:p>","DOI":"10.3390\/sym14061199","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T02:01:44Z","timestamp":1655085704000},"page":"1199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Asymmetry Evaluation of Sea Cucumber (Apostichopus japonicus) Gut and Its Surrounding Environment in the Bacterial Community"],"prefix":"10.3390","volume":"14","author":[{"given":"Jingjing","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Yeqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Luo","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Yanxia","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Zhiping","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Zhenlin","family":"Hao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]},{"given":"Jun","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China"}]},{"given":"Yaqing","family":"Chang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.1073\/pnas.1000099107","article-title":"Host-microbial symbiosis in the vertebrate gastrointestinal tract and the Lactobacillus reuteri paradigm","volume":"108","author":"Walter","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"734427","DOI":"10.1016\/j.aquaculture.2019.734427","article-title":"Comparative analysis of midgut bacterial community under Vibrio splendidus infection in Apostichopus japonicus with hindgut as a reference","volume":"513","author":"Zhang","year":"2019","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/S0140-6736(94)91708-6","article-title":"Feeding of Bifidobacterium bifidum and Streptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus","volume":"344","author":"Saavedra","year":"1995","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1134\/S0026261716010112","article-title":"Gut bacterial diversity of farmed sea cucumbers Apostichopus japonicus with different growth rates","volume":"85","author":"Sha","year":"2016","journal-title":"Microbiology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/1574-6941.12133","article-title":"Response of bacterioplankton to a glucose gradient in the absence of lysis and grazing","volume":"85","author":"Zhang","year":"2013","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.fsi.2014.04.001","article-title":"Effect of intestinal autochthonous probiotics isolated from the gut of sea cucumber (Apostichopus japonicus) on immune response and growth of A. japonicus","volume":"38","author":"Chi","year":"2014","journal-title":"Fish Shellfish. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"18206","DOI":"10.1038\/srep18206","article-title":"The impact of rearing environment on the development of gut microbiota in tilapia larvae","volume":"5","author":"Giatsis","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100629","DOI":"10.1016\/j.aqrep.2021.100629","article-title":"Influence of environmental factors and bacterial community diversity in pond water on health of chinese perch through gut microbiota change","volume":"20","author":"Li","year":"2021","journal-title":"Aquac. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1002\/(SICI)1097-010X(19980701)281:4<288::AID-JEZ5>3.0.CO;2-K","article-title":"Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrima Selenka (Holothuroidea: Echinodermata)","volume":"281","author":"Estrada","year":"1998","journal-title":"J. Exp. Zool."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1111\/are.13654","article-title":"Characterization of the bacterial community in dif-ferent parts of the gut of sea cucumber (Apostichopus Japonicus) and its variation during gut regeneration","volume":"49","author":"Wang","year":"1987","journal-title":"Aquac. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1128\/mr.59.1.143-169.1995","article-title":"Phylogenetic identification and in situ detection of individual microbial cells without cultivation","volume":"59","author":"Amann","year":"1995","journal-title":"Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1007\/s00343-019-8308-5","article-title":"High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics","volume":"37","author":"Zhao","year":"2019","journal-title":"J. Oceanol. Limnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S1074-5521(98)90108-9","article-title":"Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products","volume":"5","author":"Handelsman","year":"1998","journal-title":"Chem. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"735498","DOI":"10.1016\/j.aquaculture.2020.735498","article-title":"Temporal dynamics of bacterial communities in the water and sediments of sea cucumber (Apostichopus japonicus) culture ponds","volume":"528","author":"Zhao","year":"2020","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11070","DOI":"10.1073\/pnas.0504978102","article-title":"Obesity alters gut microbial ecology","volume":"102","author":"Ley","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1038\/ng.3663","article-title":"The effect of host genetics on the gut microbiome","volume":"48","author":"Bonder","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Gao, F., Li, F., Jie, T., Yan, J., Sun, H., and Badger, J.H. (2014). Bacterial community composition in the gut content and ambient sediment of sea cucumber Apostichopus japonicus revealed by 16S rRNA gene pyrosequencing. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0100092"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1111\/are.14002","article-title":"Microbial communities in sea cucumber (Apostichopus japonicus) culture pond and the effects of environmental factors","volume":"50","author":"Xu","year":"2019","journal-title":"Aquac. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e00489-20","DOI":"10.1128\/AEM.00489-20","article-title":"Sea cucumber intestinal regeneration reveals deterministic assembly of the gut microbiome","volume":"86","author":"Weigel","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial signature of dysbiosis in gut microbiota","volume":"33","author":"Shin","year":"2015","journal-title":"Trends Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1128\/AEM.66.4.1692-1697.2000","article-title":"Natural assemblages of marine Proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter","volume":"66","author":"Cottrell","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1128\/AEM.68.1.201-210.2002","article-title":"New degenerate Cytophaga-flexibacter-bacteroides-specific 16S ribosomal DNA-targeted oligonucleotide probes reveal high bacterial diversity in river Taff Epilithon","volume":"68","author":"Weightman","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1126\/science.1109051","article-title":"Glycan foraging in vivo by an intestine-adapted bacterial symbiont","volume":"307","author":"Sonnenburg","year":"2005","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1099\/ijs.0.02763-0","article-title":"Formosa algae gen. Nov. sp. Nov. a novel member of the family Flavobacteriaceae","volume":"54","author":"Ivanova","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1099\/ijs.0.63875-0","article-title":"Formosa agariphila sp. nov. a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa","volume":"56","author":"Nedashkovskaya","year":"2006","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/bit.24994","article-title":"Regulated expression of polysaccharide utilization and capsular biosynthesis loci in biofilm and planktonic Bacteroides thetaiotaomicron during growth in chemostats","volume":"111","author":"TerAvest","year":"2013","journal-title":"Biotechnol. Bioeng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.aquaculture.2008.10.015","article-title":"Isolation and pathogenicity of pathogens from skin ulceration disease and viscera ejection syndrome of the sea cucumber","volume":"287","author":"Deng","year":"2009","journal-title":"Apostichopus Japonicus. Aquaculture"},{"key":"ref_28","first-page":"953","article-title":"Use of macroalgae supplemented with probiotics in the Haliotis rufescens (Swainson, 1822) culture in Northern Chile","volume":"42","author":"Carvajal","year":"2010","journal-title":"Aquac. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.aquaculture.2016.12.028","article-title":"Vibrio sp. 33 a potential bacterial antagonist of Vibrio splendidus pathogenic to sea cucumber (Apostichopus japonicus)","volume":"470","author":"Liu","year":"2017","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1038\/ismej.2014.261","article-title":"Chemotaxis by natural populations of coral reef bacteria","volume":"9","author":"Tout","year":"2015","journal-title":"ISME J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e01748-15","DOI":"10.1128\/mBio.01748-15","article-title":"What a dinner party! Mechanisms and functions of interkingdom signaling in host-pathogen associations","volume":"7","author":"Kendall","year":"2016","journal-title":"MBio"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/s12602-018-9511-y","article-title":"Probiotic Lactobacillus johnsonii BS15 promotes growth performance, intestinal immunity, and gut microbiota in Piglets","volume":"12","author":"Xin","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1053\/j.gastro.2006.11.022","article-title":"Soluble proteins produced by probiotic bacteria regulate intestinal epithelial cell survival and growth","volume":"132","author":"Yan","year":"2007","journal-title":"Gastroenterology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2430","DOI":"10.1128\/AEM.67.6.2430-2435.2001","article-title":"Characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish","volume":"67","author":"Nikoskelainen","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00203-010-0575-7","article-title":"Bacterial energy taxis: A global strategy?","volume":"192","author":"Schweinitzer","year":"2010","journal-title":"Arch. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lamendella, R., Santo Domingo, J.W., Ghosh, S., Martinson, J., and Oerther, D.B. (2011). Comparative fecal metagenomics unveils unique functional capacity of the swine gut. BMC Microbiol., 11.","DOI":"10.1186\/1471-2180-11-103"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Erickson, A.R., Cantarel, B.L., Lamendella, R., Darzi, Y., Mongodin, E.F., Pan, C., Shah, M., Halfvarson, J., Tysk, C., and Henrissat, B. (2012). Integrated metagenomics\/metaproteomics reveals human host-microbiota signatures of Crohn\u2019s disease. PLoS ONE., 7.","DOI":"10.1371\/journal.pone.0049138"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.aquaculture.2015.05.038","article-title":"Effects of dietary carbohydrate level on growth, biochemical composition and glucose metabolism of juvenile sea cucumber Apostichopus japonicus (Selenka)","volume":"448","author":"Xia","year":"2015","journal-title":"Aquaculture"},{"key":"ref_39","first-page":"254","article-title":"Expression and biochemical characterization and substrate specificity of the fucoidanase from Formosa algae","volume":"27","author":"Silchenko","year":"2016","journal-title":"Glycobiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.hal.2008.08.015","article-title":"Coastal nitrogen pollution: A review of sources and trends globally and regionally","volume":"8","author":"Howarth","year":"2009","journal-title":"Harmful Algae"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/BF00694263","article-title":"Chloride uptake in freshwater teleosts and its relationship to nitrite uptake and toxicity","volume":"156","author":"Williams","year":"1986","journal-title":"J. Comp. Physiol. B"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jin, H., Zhang, X., Li, K., Niu, Y., Guo, M., Hu, C., Wan, X., Gong, Y., and Huang, F. (2014). Direct bio-utilization of untreated rapeseed meal for effective iturin a production by Bacillus subtilis in submerged fermentation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111171"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1097\/MCO.0b013e3280115d36","article-title":"Emerging aspects of gut sulfur amino acid metabolism","volume":"10","author":"Burrin","year":"2007","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_44","first-page":"4215","article-title":"Dietary cysteine reduces the methionine requirement by an equal proportion in both parenterally and enterally fed piglets","volume":"133","author":"Shoveller","year":"2003","journal-title":"Nutr. Requir."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.phrs.2012.11.005","article-title":"Intestinal luminal nitrogen metabolism: Role of the gut microbiota and consequences for the host","volume":"68","author":"Davila","year":"2013","journal-title":"Pharmacol. Res."}],"container-title":["Symmetry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-8994\/14\/6\/1199\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:27:28Z","timestamp":1760138848000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-8994\/14\/6\/1199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,10]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["sym14061199"],"URL":"https:\/\/doi.org\/10.3390\/sym14061199","relation":{},"ISSN":["2073-8994"],"issn-type":[{"value":"2073-8994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,10]]}}}