{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T23:05:35Z","timestamp":1771369535528,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Princess Nourah bint Abdulrahman University Researchers Supporting Project","award":["PNURSP2022R25"],"award-info":[{"award-number":["PNURSP2022R25"]}]},{"name":"Princess Nourah bint Abdulrahman University Researchers Supporting Project","award":["RGP. 2\/113\/43"],"award-info":[{"award-number":["RGP. 2\/113\/43"]}]},{"name":"Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia &amp; Deanship of Scientific Research, King Khalid University, KSA","award":["PNURSP2022R25"],"award-info":[{"award-number":["PNURSP2022R25"]}]},{"name":"Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia &amp; Deanship of Scientific Research, King Khalid University, KSA","award":["RGP. 2\/113\/43"],"award-info":[{"award-number":["RGP. 2\/113\/43"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Symmetry"],"abstract":"<jats:p>Hydrazones are regarded as a distinctive category of organic compounds because of their tremendous characteristics and potential uses in analytical, chemical, and medicinal chemistry. In the present study, a new series of Hydrazone Derivatives bearing cis-(4-chlorostyryl) amide moiety were designed and synthesized. In vitro cytotoxicity screening showed that compounds 3i, 3l, 3m, and 3n revealed potent anticancer activity against MCF-7 cancer cell line with IC50 values between 2.19\u20134.37 \u03bcM compared with Staurosporin as a reference compound. The antiproliferative activity of these compounds appears to be correlated well with their ability to inhibit the VEGFR-2 kinase enzyme. Activation of the damage response pathway leads to cellular cycle arrest at the G1 phase. Fluorochrome Annexin V\/PI staining indicated that cell death proceeds through the apoptotic pathway mechanism. The mechanistic pathway was confirmed by a significant increase in the level of active caspase 9 compared with control untreated MCF-7 cells.<\/jats:p>","DOI":"10.3390\/sym14112457","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T03:09:30Z","timestamp":1669000170000},"page":"2457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Design, Synthesis, and In Vitro Antiproliferative Screening of New Hydrazone Derivatives Containing cis-(4-Chlorostyryl) Amide Moiety"],"prefix":"10.3390","volume":"14","author":[{"given":"Tarfah","family":"Al-Warhi","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia"}]},{"given":"Leena S.","family":"Alqahtani","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Science, University of Jeddah, Jeddah 23445, Saudi Arabia"}]},{"given":"Matokah","family":"Abualnaja","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Applied Science, Umm Al-Qura University, Makkah Al Mukarramah 24381, Saudi Arabia"}]},{"given":"Saba","family":"Beigh","sequence":"additional","affiliation":[{"name":"Department of Public Health, Faculty of Applied Medical Sciences, Albaha University, Albaha 65431, Saudi Arabia"}]},{"given":"Ola A.","family":"Abu Ali","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}]},{"given":"Fahmy G.","family":"Elsaid","sequence":"additional","affiliation":[{"name":"Biology Department, Science College, King Khalid University, Abha 61421, Saudi Arabia"},{"name":"Zoology Department, Faculty of Science, Mansoura University, P.O. Box 70, Mansoura 35516, Egypt"}]},{"given":"Ali A.","family":"Shati","sequence":"additional","affiliation":[{"name":"Biology Department, Science College, King Khalid University, Abha 61421, Saudi Arabia"}]},{"given":"Rasha Mohammed","family":"Saleem","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha 65431, Saudi Arabia"}]},{"given":"Ali Hassan Ahmed","family":"Maghrabi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 24381, Saudi Arabia"}]},{"given":"Amani Abdulrahman","family":"Alharthi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}]},{"given":"Amal","family":"Alyamani","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2916-0254","authenticated-orcid":false,"given":"Eman","family":"Fayad","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9056-0228","authenticated-orcid":false,"given":"Ali H.","family":"Abu Almaaty","sequence":"additional","affiliation":[{"name":"Zoology Department, Faculty of Science, Port Said University, Port Said 42526, Egypt"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2026-7373","authenticated-orcid":false,"given":"Islam","family":"Zaki","sequence":"additional","affiliation":[{"name":"Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt"}]},{"given":"Shaimaa","family":"Hamouda","sequence":"additional","affiliation":[{"name":"Zoology Department, Faculty of Science, Mansoura University, P.O. 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