{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T18:38:53Z","timestamp":1781721533443,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agriculture"],"abstract":"<jats:p>Among the waste by-products generated by the fruit industry (peels, seeds, and skins), fruit peel constitutes the major component. It is estimated that fruit peel accounts for at least 20% of the fresh fruit weight. Fruit peels are considered as major sources of dietary fiber and anticipated to be successfully utilized as prebiotics. This study examined the chemical composition, functional properties and the prebiotic effects of three major tropical fruit peels (apple, banana and mango). The prebiotic effect was tested using three commercial probiotic strains (Lactobacillus rhamnosus, L. casei and Bifidobacterium lactis) individually and in combination. Each probiotic culture was fortified with different concentration (0%, 2% and 4%) of selected fruit peel powder (FPP). Results revealed that all tested FPP significantly (p &lt; 0.05) enhanced the probiotics viable counts, which reached &gt;10 logs after 24 h of incubation. However, the concentration of 2% and 4% FPP showed no significant differences (p &gt; 0.05) on the probiotic viable counts. Additionally, the prebiotic effects of FPP were the same when applied to individual and mixed cultures. This investigation demonstrated that small amount (2%) of apple, banana and mango peel powder could be successfully utilized as prebiotics to enhance the growth of lactic acid bacteria (LAB). Additionally, the studied physical and chemical characteristics of FPP demonstrated their potential applications in the food and pharmaceutical industries as functional ingredients.<\/jats:p>","DOI":"10.3390\/agriculture11070584","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T11:01:38Z","timestamp":1624532498000},"page":"584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Utilization of Mango, Apple and Banana Fruit Peels as Prebiotics and Functional Ingredients"],"prefix":"10.3390","volume":"11","author":[{"given":"Hafza Fasiha","family":"Zahid","sequence":"first","affiliation":[{"name":"School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1367-7999","authenticated-orcid":false,"given":"Chaminda Senaka","family":"Ranadheera","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9902-3426","authenticated-orcid":false,"given":"Zhongxiang","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6896-5509","authenticated-orcid":false,"given":"Said","family":"Ajlouni","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"ref_1","first-page":"24","article-title":"Utilization of fruit processing by-products for industrial applications: A review","volume":"2","author":"Kodagoda","year":"2017","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1111\/1541-4337.12330","article-title":"Fruit and vegetable waste: Bioactive compounds, their extraction, and possible utilization","volume":"17","author":"Sagar","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_3","first-page":"1","article-title":"Review: Food industry by-products used as a functional food ingredients","volume":"6","author":"Helkar","year":"2017","journal-title":"Int. J. Waste Resour."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1080\/15428052.2018.1489323","article-title":"Production of low-calorie ice cream utilizing apple peel and pulp","volume":"17","author":"Lazari","year":"2019","journal-title":"J. Culin. Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13460","DOI":"10.1111\/jfpp.13460","article-title":"Effect of coconut milk, skim milk powder, and banana pulp on sensory and functional properties of coconut curd and its applicability as a carrier for probiotic microorganisms","volume":"42","author":"Chauhan","year":"2018","journal-title":"J. Food Process. Preserv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1007\/s13197-013-1184-7","article-title":"Quality characteristics of no added sugar ready to drink milk supplemented with mango pulp","volume":"52","author":"Bajwa","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1007\/s11947-018-2117-2","article-title":"Dietary fiber concentrates from fruit and vegetable by-products: Processing, modification, and application as functional ingredients","volume":"11","year":"2018","journal-title":"Food Bioprocess Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1111\/ijfs.12113","article-title":"In vitro evaluation of the fermentation of added-value agroindustrial by-products: Cactus pear (Opuntia ficus-indica L.) peel and pineapple (Ananas comosus) peel as functional ingredients","volume":"48","author":"Totosaus","year":"2013","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1080\/87559129.2015.1094815","article-title":"Agro-industrial potential of the mango peel based on its nutritional and functional properties","volume":"32","year":"2016","journal-title":"Food Rev. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.lwt.2014.05.030","article-title":"Passion fruit (Passiflora edulis) peel increases colonic production of short-chain fatty acids in wistar rats","volume":"59","author":"Cazarin","year":"2014","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1021\/jf403288r","article-title":"Oral administration of Lactobacillus fermentum i5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets","volume":"62","author":"Liu","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3712","DOI":"10.1002\/jsfa.8232","article-title":"Potential prebiotic properties of cashew apple (Anacardium occidentale L.) agro-industrial byproduct on lactobacillus species","volume":"97","author":"Duarte","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","unstructured":"Horwitz, W. (2010). Official Methods of Analysis of Aoac International. Volume I, Agricultural Chemicals, Contaminants, Drugs\/Edited by William Horwitz, AOAC International 1997."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Peng, D., Zahid, H.F., Ajlouni, S., Dunshea, F.R., and Suleria, H.A. (2019). LC-ESI-QTOF\/MS profiling of australian mango peel by-product polyphenols and their potential antioxidant activities. Processes, 7.","DOI":"10.3390\/pr7100764"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ali, A., Wu, H., Ponnampalam, E.N., Cottrell, J.J., Dunshea, F.R., and Suleria, H.A.R. (2021). Comprehensive profiling of most widely used spices for their phenolic compounds through LC-ESI-QTOF-MS2 and their antioxidant potential. Antioxidants, 10.","DOI":"10.3390\/antiox10050721"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Iqbal, Y., Ponnampalam, E.N., Suleria, H.A.R., Cottrell, J.J., and Dunshea, F.R. (2021). LC-ESI\/QTOF-MS profiling of Chicory and Lucrene polyphenols and their antioxidant activities. Antioxidants, 10.","DOI":"10.3390\/antiox10060932"},{"key":"ref_17","first-page":"2348","article-title":"Physicochemical and functional properties of pulp and peel flour of dried green and ripe banana (Cavendish)","volume":"4","author":"Salih","year":"2017","journal-title":"Int. J. Res. Agric. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.21475\/ajcs.18.12.11.p1489","article-title":"Peel colour, anthocyanin, tss content and sensory evaluation of some common fruits: A comparative study","volume":"12","author":"Khandaker","year":"2018","journal-title":"Aust. J. Crop Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.foodres.2017.04.026","article-title":"The impact of fruit and soybean by-products and amaranth on the growth of probiotic and starter microorganisms","volume":"97","author":"Vieira","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1038\/ismej.2016.42","article-title":"Consortia of low-abundance bacteria drive sulfate reduction-dependent degradation of fermentation products in peat soil microcosms","volume":"10","author":"Hausmann","year":"2016","journal-title":"ISME J."},{"key":"ref_21","first-page":"1","article-title":"Determination of mineral, trace element, and pesticide levels in honey samples originating from different regions of malaysia compared to manuka honey","volume":"2014","author":"Moniruzzaman","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.foodchem.2006.09.006","article-title":"Effects of the stage of maturation and varieties on the chemical composition of banana and plantain peels","volume":"103","author":"Emaga","year":"2007","journal-title":"Food Chem."},{"key":"ref_23","first-page":"12","article-title":"Chemical composition of some selected fruit peels","volume":"4","author":"Romelle","year":"2016","journal-title":"Eur. J. Food Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"934","DOI":"10.3923\/pjn.2013.934.942","article-title":"Chemical profiling of different mango peel varieties","volume":"12","author":"Imran","year":"2013","journal-title":"Pak. J. Nutr."},{"key":"ref_25","first-page":"893","article-title":"A comprehensive analysis of the composition, health benefits, and safety of apple pomace","volume":"76","author":"Skinner","year":"2018","journal-title":"Nutr. Rev."},{"key":"ref_26","first-page":"308","article-title":"Proximate composition, mineral contents and fatty acid composition of the different parts and dried peels of tropical fruits cultivated in brazil","volume":"28","author":"Morais","year":"2017","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"506","DOI":"10.3945\/an.112.002154","article-title":"Health benefits of fruits and vegetables","volume":"3","author":"Slavin","year":"2012","journal-title":"Adv. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.jff.2012.11.017","article-title":"Mango peel dietary fibre: Composition and associated bound phenolics","volume":"5","author":"Ajila","year":"2013","journal-title":"J. Funct. Food"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Barber, T.M., Kabisch, S., Pfeiffer, A.F.H., and Weickert, M.O. (2020). The health benefits of dietary fibre. Nutrients, 12.","DOI":"10.3390\/nu12103209"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lyu, F., Luiz, S.F., Azeredo, D.R.P., Cruz, A.G., Ajlouni, S., and Ranadheera, C.S. (2020). Apple pomace as a functional and healthy ingredient in food products: A review. Processes, 8.","DOI":"10.3390\/pr8030319"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/1471-0307.12630","article-title":"Microbial, physico-chemical and sensory characteristics of mango juice enriched probiotic dairy drinks","volume":"73","author":"Ryan","year":"2019","journal-title":"Int. J. Dairy Technol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Suleria, H.A.R., Barrow, C.J., and Dunshea, F.R. (2020). Screening and characterization of phenolic compounds and their antioxidant capacity in different fruit peels. Foods, 9.","DOI":"10.3390\/foods9091206"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1016\/j.foodchem.2007.02.010","article-title":"The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. And Juniperus phoenicea L. Fruit extracts","volume":"105","author":"Hayouni","year":"2007","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.aca.2011.07.018","article-title":"Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review","volume":"703","author":"Mustafa","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/jsfa.1933","article-title":"Optimization of extraction of phenolic antioxidants from peanut skins","volume":"85","author":"Nepote","year":"2004","journal-title":"J. Sci. Food Agric."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e12438","DOI":"10.1111\/jfpe.12438","article-title":"Optimizing the Homogenizer-Assisted Extraction (HAE) of total phenolic compounds from banana peel","volume":"40","author":"Pereira","year":"2017","journal-title":"J. Food Process Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1080\/10942912.2010.535187","article-title":"Potential of selected tropical fruit peels as dietary fiber in functional foods","volume":"18","author":"Wanlapa","year":"2015","journal-title":"Int. J. Food Prop."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.foodchem.2004.04.036","article-title":"Fibre concentrates from apple pomace and citrus peel as potential fibre sources for food enrichment","volume":"91","author":"Figuerola","year":"2005","journal-title":"Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.fshw.2015.10.001","article-title":"Functional properties, phenolic constituents and antioxidant potential of industrial apple pomace for utilization as active food ingredient","volume":"4","author":"Rana","year":"2015","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.foodchem.2010.06.077","article-title":"Dietary fibre and fibre-rich by-products of food processing: Characterisation, technological functionality and commercial applications: A review","volume":"124","author":"Elleuch","year":"2011","journal-title":"Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1016\/j.foodchem.2007.10.007","article-title":"Characterization of a fibre-rich powder prepared by liquefaction of unripe banana flour","volume":"107","author":"Tovar","year":"2008","journal-title":"Food Chem."},{"key":"ref_42","first-page":"349","article-title":"Physicochemical properties of banana peel flour as influenced by variety and stage of ripeness: Multivariate statistical analysis","volume":"3","author":"Alkarkhi","year":"2010","journal-title":"Asian J. Food Agro-Ind."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nfs.2019.10.001","article-title":"Comparison of the physico-chemical and functional properties of mango kernel flour with wheat flour and development of mango kernel flour based composite cakes","volume":"17","author":"Das","year":"2019","journal-title":"NFS J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.foodchem.2005.09.013","article-title":"Factors affecting instant properties of powdered cocoa beverages","volume":"100","author":"Shittu","year":"2007","journal-title":"Food Chem."},{"key":"ref_45","first-page":"177","article-title":"Physicochemical properties and nutritional compositions of foamed banana powders (Pisang awak, musa sapientum L.) dehydrated by various drying methods","volume":"13","author":"Naknaen","year":"2015","journal-title":"Walailak J. Sci. Technol. (WJST)"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/19476337.2012.703693","article-title":"Comparison of three drying processes to obtain an apple peel food ingredient","volume":"11","author":"Almonacid","year":"2013","journal-title":"CyTA-J. Food"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Coelho, E.M., de Souza, M.E.A.O., Corr\u00eaa, L.C., Viana, A.C., de Azev\u00eado, L.C., and dos Santos Lima, M. (2019). Bioactive compounds and antioxidant activity of mango peel liqueurs (Mangifera indica L.) produced by different methods of maceration. Antioxidants, 8.","DOI":"10.3390\/antiox8040102"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"165","DOI":"10.2174\/2212711901666131203000741","article-title":"Evaluation of the browning kinetics for bananas and pears submitted to convective drying","volume":"1","author":"Guine","year":"2014","journal-title":"Curr. Biochem. Eng."},{"key":"ref_49","first-page":"19","article-title":"Evaluation of banana peel waste as growth medium for probiotic lactobacillus species","volume":"8","author":"Farees","year":"2017","journal-title":"Int. J. Appl. Biol. Pharm. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1504\/IJNBM.2016.086107","article-title":"Growth and survival of lactobacillus casei in rice bran and banana peel medium","volume":"6","author":"Esivan","year":"2016","journal-title":"Int. J. Nano Biomater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1002\/bit.260261103","article-title":"Cybernetic modeling of microbial growth on multiple substrates","volume":"26","author":"Kompala","year":"1984","journal-title":"Biotechnol. Bioeng."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.foodres.2017.12.024","article-title":"Prebiotic effect of predigested mango peel on gut microbiota assessed in a dynamic in vitro model of the human colon (Tim-2)","volume":"118","author":"Venema","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"84","DOI":"10.15446\/dyna.v85n207.70578","article-title":"Evaluation of viability of probiotic bacteria in mango (Mangifera indica L. Cv.\u201cTommy atkins\u201d) beverage","volume":"85","year":"2018","journal-title":"Dyna"},{"key":"ref_54","first-page":"547","article-title":"Lactic acid fermentation of apricot juice by mono- and mixed cultures of probiotic Lactobacillus and Bifidobacterium strains","volume":"27","author":"Bujna","year":"2018","journal-title":"Food Sci. Biotechnol."}],"container-title":["Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0472\/11\/7\/584\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:23:19Z","timestamp":1760163799000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0472\/11\/7\/584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,24]]},"references-count":54,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["agriculture11070584"],"URL":"https:\/\/doi.org\/10.3390\/agriculture11070584","relation":{},"ISSN":["2077-0472"],"issn-type":[{"value":"2077-0472","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,24]]}}}