{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:00:28Z","timestamp":1777417228019,"version":"3.51.4"},"reference-count":71,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202617"],"award-info":[{"award-number":["32202617"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["323RC417"],"award-info":[{"award-number":["323RC417"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["32202617"],"award-info":[{"award-number":["32202617"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["323RC417"],"award-info":[{"award-number":["323RC417"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>As a crucial staple crop in agricultural production, maize is extensively cultivated worldwide and plays a pivotal role in ensuring global food security. However, the significant deficiency of essential amino acids (EAA) and conditionally essential amino acids (CEAA), such as lysine (Lys), tryptophan (Trp), methionine (Met), and cysteine (Cys), leads to an imbalance of amino acids in the grain. This study investigates the regulatory mechanism of sulfur (S) application for regulating the amino acid balance of maize grains. The results demonstrate that S application has substantial effects on both the maize yield and nutritional quality. The S application resulted in an increase in maize yield by simultaneously enhancing the grain number per ear (GN) and 100-grain weight (GW), while S application elevated protein concentration through the augmentation of Cys concentration in maize grains. Furthermore, the Cys in grains optimizes the amino acid balance by regulating the ratio of other amino acids, thereby enhancing the nutritional quality of maize while ensuring a steady increase in protein concentration, simultaneously. Overall, the S application at 60\u201390 kg ha\u22121 synergistically improved both the yield and nutritional quality of maize, meeting the requirements for sustainable development in maize production. The findings offer a novel theoretical foundation and nutrient management approach for achieving high-yield and superior-quality maize production.<\/jats:p>","DOI":"10.3390\/agronomy13122912","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T07:56:10Z","timestamp":1701071770000},"page":"2912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sulfur Application Improves the Nutritional Quality of Maize by Regulating the Amino Acid Balance of Grains"],"prefix":"10.3390","volume":"13","author":[{"given":"Honglin","family":"Wang","sequence":"first","affiliation":[{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya 572025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Cui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sustainable Utilization of Soil Resources in The Commodity Grain Bases of Jilin Province, College of Resource and Environmental Sciences, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianan","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya 572025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huadong","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya 572025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8163-805X","authenticated-orcid":false,"given":"Shuoran","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya 572025, China"},{"name":"Key Laboratory of Sustainable Utilization of Soil Resources in The Commodity Grain Bases of Jilin Province, College of Resource and Environmental Sciences, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"ref_1","unstructured":"SDG (Sustainable Development Goal) (2020, February 07). Sustainable Development Goals. Available online: https:\/\/sustainabledevelopment.un.org\/sdg2."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"128723","DOI":"10.1016\/j.foodchem.2020.128723","article-title":"Missing association between nutrient concentrations in leaves and edible parts of food crops\u2014A neglected food security issue","volume":"345","author":"Fischer","year":"2021","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Reeds, P.J. (1996). Amino Acid Metabolism and Therapy in Health and Nutritional Disease, CRC Press.","DOI":"10.1093\/ajcn\/64.4.665"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1093\/jn\/128.5.789","article-title":"Old and new substrates in clinical nutrition","volume":"128","year":"1998","journal-title":"J. Nutr."},{"key":"ref_5","first-page":"3747","article-title":"Molecular mechanism of methionine differentiation in high and low methionine maize lines","volume":"10","author":"Ali","year":"2011","journal-title":"Afr. J. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/s41467-019-14023-2","article-title":"Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize","volume":"11","author":"Li","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_7","unstructured":"EPA (Environmental Protection Agency) (2020, April 06). Predicting the Impact of Coexistence-Guided, Genetically Modified Cropping on Irish Biodiversity. STRIVE Environmental Protection Agency Program 2007\u20132013. Available online: http:\/\/www.epa.ie\/pubs\/reports\/research\/biodiversity\/STRIVE_39_Mullins_Genetics_322web.pdf."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Amegbor, I., van Biljon, A., Shargie, N., Tarekegne, A., and Labuschagne, M. (2022). Identifying quality protein maize inbred lines for improved nutritional value of maize in Southern Africa. Foods, 11.","DOI":"10.3390\/foods11070898"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100327","DOI":"10.1016\/j.gfs.2019.100327","article-title":"Maize agro-food systems to ensure food and nutrition security in reference to the Sustainable Development Goals","volume":"25","author":"Tanumihardjo","year":"2019","journal-title":"Glob. Food Secur."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1007\/s00122-009-1115-1","article-title":"Wide variability in kernel composition, seed characteristics, and zein profiles among diverse maize inbreds, landraces, and teosinte","volume":"119","author":"Bodnar","year":"2009","journal-title":"Theor. Appl. Genet."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1203S","DOI":"10.1093\/ajcn\/59.5.1203S","article-title":"Plant proteins in relation to human protein and amino acid nutrition","volume":"59","author":"Young","year":"1994","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","first-page":"576","article-title":"Seed storage proteins and approaches for improvement of their nutritional quality by genetic engineering","volume":"79","author":"Mandal","year":"2000","journal-title":"Curr. Sci. India"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1136\/bmj.326.7381.146","article-title":"Management of the severely malnourished child: Perspective from developing countries","volume":"326","author":"Bhan","year":"2003","journal-title":"Brit. Med. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s12298-019-00724-x","article-title":"Temporal profiling of essential amino acids in developing maize kernel of normal, opaque-2 and QPM germplasm","volume":"26","author":"Sethi","year":"2020","journal-title":"Physiol. Mol. Biol. Plants"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"240","DOI":"10.3389\/fpls.2014.00240","article-title":"Proteome balancing of the maize seed for higher nutritional value","volume":"5","author":"Wu","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/pbr.12923","article-title":"Quality protein maize (QPM): Importance, genetics, timeline of different events, breeding strategies and varietal adoption","volume":"140","author":"Maqbool","year":"2021","journal-title":"Plant Breed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1093\/jxb\/erz319","article-title":"Sulfur nutrition: Impacts on plant development, metabolism, and stress responses","volume":"70","author":"Kopriva","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3389\/fpls.2014.00442","article-title":"Transporters in plant sulfur metabolism","volume":"5","author":"Gigolashvili","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zenda, T., Liu, S.T., Dong, A.Y., and Duan, H.J. (2021). Revisiting Sulphur-the once neglected nutrient: It\u2019s roles in plant growth, metabolism, stress tolerance and crop production. Agriculture, 11.","DOI":"10.3390\/agriculture11070626"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hossain, M.A., Mostofa, M.G., Diaz-Vivancos, P., Burritt, D.J., Fujita, M., and Tran, L.P. (2017). Glutathione in Plant Growth, Development, and Stress Tolerance, Springer.","DOI":"10.1007\/978-3-319-66682-2"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.jgg.2016.07.001","article-title":"Sulfur metabolism and its manipulation in crops","volume":"43","author":"Koprivova","year":"2016","journal-title":"J. Genet. Genom."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/sum.12250","article-title":"Do UK crops and grassland require greater inputs of sulphur fertilizer in response to recent and forecast reductions in sulphur emissions and deposition?","volume":"32","author":"Webb","year":"2016","journal-title":"Soil Use Manag."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2485","DOI":"10.2134\/agronj2019.02.0095","article-title":"World sulfur use efficiency for cereal crops","volume":"111","author":"Aula","year":"2019","journal-title":"Agron. J."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Cai, J., Zang, F., Xin, L., Zhou, Q., Wang, X., Zhong, Y., Huang, M., Dai, T., and Jiang, D. (2022). Effects of cysteine and inorganic sulfur applications at different growth stages on grain protein and end-use quality in wheat. Foods, 11.","DOI":"10.3390\/foods11203252"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/jpln.200900037","article-title":"Sulfur in soils","volume":"172","author":"Scherer","year":"2009","journal-title":"J. Plant Nutr. Soil Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/s41561-020-0620-3","article-title":"A shift in sulfur-cycle manipulation from atmospheric emissions to agricultural additions","volume":"13","author":"Hinckley","year":"2020","journal-title":"Nat. Geosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1097\/SS.0b013e31821f0816","article-title":"Comparison of ammonium sulfate with other nitrogen and sulfur fertilizers in increasing crop production and minimizing environmental impact: A review","volume":"176","author":"Chien","year":"2011","journal-title":"Soil Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1080\/00103624.2020.1869773","article-title":"Maize response to sulfur fertilizer in three Iowa Soils","volume":"52","author":"Kovar","year":"2021","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0065-2113(09)01002-5","article-title":"Soil sulfur cycling in temperate agricultural systems","volume":"102","author":"Eriksen","year":"2009","journal-title":"Adv. Agron."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"576718","DOI":"10.3389\/fpls.2020.576718","article-title":"Synergistic regulation of nitrogen and sulfur on redox balance of maize leaves and amino acids balance of grains","volume":"11","author":"Liu","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/j.1399-3054.1986.tb03404.x","article-title":"Chaises in accumulation of seed nitrogen compounds in maize under conditions of sulphur deficiency","volume":"68","author":"Baudet","year":"1986","journal-title":"Physiol. Plant."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/pbi.12025","article-title":"Fortifying plants with the essential amino acids lysine and methionine to improve nutritional quality","volume":"11","author":"Galili","year":"2013","journal-title":"Plant Biotechnol. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1002\/csc2.20983","article-title":"Development of maize inbred lines with elevated grain methionine concentration from a high methionine population","volume":"63","author":"Hintch","year":"2023","journal-title":"Crop Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/1471-2229-12-77","article-title":"Balancing of sulfur storage in maize seed","volume":"12","author":"Wu","year":"2012","journal-title":"BMC Plant Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11386","DOI":"10.1073\/pnas.1714805114","article-title":"Engineering sulfur storage in maize seed proteins without apparent yield loss","volume":"114","author":"Planta","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"103189","DOI":"10.1016\/j.jcs.2021.103189","article-title":"Simultaneous improvement of protein concentration and amino acid balance in maize grains by coordination application of nitrogen and sulfur","volume":"99","author":"Liu","year":"2021","journal-title":"J. Cereal Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107593","DOI":"10.1016\/j.fcr.2019.107593","article-title":"Combined effects of nitrogen and sulfur fertilization on maize growth, physiological traits, N and S uptake, and their diagnosis","volume":"242","author":"Li","year":"2019","journal-title":"Field Crop Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"807","DOI":"10.2136\/sssaj1999.634807x","article-title":"A computer program for soil textural classification","volume":"63","author":"Gerakis","year":"1999","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/nature13609","article-title":"Producing more grain with lower environmental costs","volume":"514","author":"Chen","year":"2014","journal-title":"Nature"},{"key":"ref_40","unstructured":"(2005). Animal Feeding Stuffs-Determination of Nitrogen Content and Calculation of Crude Protein Content-Part 1: Kjeldahl Method. Standard No. ISO 5983-1."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.tig.2005.02.009","article-title":"Molecular genetic approaches to developing quality protein maize","volume":"21","author":"Gibbon","year":"2005","journal-title":"Trends Genet."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3389\/fpls.2014.00276","article-title":"Recent advances in the study of prolamin storage protein organization and function","volume":"5","author":"Holding","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_43","unstructured":"WHO (World Health Organization) (2007). Joint WHO\/FAO\/UNU Expert Consultation: Protein and Amino Acid Requirements in Human Nutrition, World Health Organization. Technical Report 935."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"104775","DOI":"10.1016\/j.jfca.2022.104775","article-title":"Nutritional composition of maize grain associated with phosphorus and zinc fertilization","volume":"114","author":"Yu","year":"2022","journal-title":"J. Food Compos. Anal."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.2135\/cropsci1999.3961622x","article-title":"Post-green revolution trends in yield potential of temperate maize in the North-Central United States","volume":"39","author":"Duvick","year":"1999","journal-title":"Crop Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.fcr.2012.03.008","article-title":"Physiological perspectives of changes over time in maize yield dependency on nitrogen uptake and associated nitrogen efficiencies: A review","volume":"133","author":"Ciampitti","year":"2012","journal-title":"Field Crop Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0065-2113(05)86002-X","article-title":"The contribution of breeding to yield advances in maize (Zea mays L.)","volume":"86","author":"Duvick","year":"2005","journal-title":"Adv. Agron."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.eja.2014.09.008","article-title":"Effects of nitrogen application rate on grain yield and grain nitrogen concentration in two maize hybrids with contrasting nitrogen remobilization efficiency","volume":"62","author":"Chen","year":"2015","journal-title":"Eur. J. Agron."},{"key":"ref_49","first-page":"417","article-title":"Grain composition and amino acid content in maize cultivars representing 80 years of commercial maize varieties","volume":"51","author":"Scott","year":"2006","journal-title":"Maydica"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"107754","DOI":"10.1016\/j.fcr.2020.107754","article-title":"Improving grain yield and protein concentration of maize (Zea mays L.) simultaneously by appropriate hybrid selection and nitrogen management","volume":"249","author":"Zhang","year":"2020","journal-title":"Field Crop Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"106828","DOI":"10.1016\/j.agwat.2021.106828","article-title":"Effect of sulfur fertilizer on summer maize grain yield and soil water utilization under different irrigation patterns from anthesis to maturity","volume":"250","author":"Ma","year":"2021","journal-title":"Agric. Water Manag."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.anifeedsci.2010.05.009","article-title":"The distribution of crude protein and amino acid content in maize grain and soybean meal","volume":"159","author":"Sriperm","year":"2010","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1089\/ars.2010.3575","article-title":"How proteins form disulfide bonds","volume":"15","author":"Depuydt","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0968-0004(03)00057-4","article-title":"Disulfide bonds as switches for protein function","volume":"28","author":"Hogg","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5910","DOI":"10.1073\/pnas.1605363113","article-title":"Enhancing protein stability with extended disulfide bonds","volume":"113","author":"Liu","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.celrep.2014.01.006","article-title":"Hypothalamic eIF2\u03b1 Signaling Regulates Food Intake","volume":"6","author":"Maurin","year":"2014","journal-title":"Cell Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"132","DOI":"10.2174\/1389203719666180517100746","article-title":"Regulatory role of amino acids in pigs fed on protein-restricted diets","volume":"20","author":"Kim","year":"2019","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Wu, Y.R., and Messing, J. (2012). RNA interference can rebalance the nitrogen sink of maize seeds without losing hard endosperm. PLoS ONE, 7.","DOI":"10.1371\/annotation\/06346ecc-81e4-4cec-8923-0551781a416a"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"211","DOI":"10.2134\/agronj1961.00021962005300040001x","article-title":"Effect of nitrogen fertilizer and soil type on the amino acid content of corn grain","volume":"53","author":"MacGregor","year":"1961","journal-title":"Agron. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1002\/jsfa.2740340309","article-title":"Interactions between the kernel N sink, grain yield and protein nutritional quality of maize","volume":"34","author":"Tsai","year":"1983","journal-title":"J. Sci. Food Agric."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jsfa.2740580102","article-title":"Interrelationship of nitrogen nutrition with maize (Zea mays) grain yield, nitrogen use efficiency and grain quality","volume":"58","author":"Tsai","year":"1992","journal-title":"J. Sci. Food Agric."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"574","DOI":"10.17221\/288\/2010-PSE","article-title":"Effect of nitrogen fertilization on metabolisms of essential and non-essential amino acids in field-grown grain maize (Zea mays L.)","volume":"56","author":"Bunka","year":"2010","journal-title":"Plant Soil Environ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1080\/01904160903152616","article-title":"Response of wheat cultivars to nitrogen and sulfur for crop yield, nitrogen use efficiency, and protein quality in the semiarid region","volume":"32","author":"Habtegebrial","year":"2009","journal-title":"J. Plant Nutr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jcs.2015.02.002","article-title":"Influence of sulphur application on hordein composition and malting quality of barley (hordeum vulgare L.) in northern European growing conditions","volume":"62","author":"Holopainen","year":"2015","journal-title":"J. Cereal Sci."},{"key":"ref_65","unstructured":"Sheridan, W.F. (1982). Maize for Biological Research, University of North Dakota Press."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Shewry, P.R., and Casey, R. (1999). Seed Proteins, Kluwer Academic Publishers.","DOI":"10.1007\/978-94-011-4431-5"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0733-5210(87)80015-2","article-title":"Proposed nomenclature for the alcohol-soluble proteins (zeins) of maize (Zea mays L.)","volume":"5","author":"Esen","year":"1987","journal-title":"J. Cereal Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/0378-1119(88)90053-4","article-title":"Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize","volume":"71","author":"Kirihara","year":"1988","journal-title":"Gene"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1046\/j.1365-313X.1995.08030359.x","article-title":"Determinants of the high-methionine trait in wild and exotic germplasm may have escaped selection during early cultivation of maize","volume":"8","author":"Swarup","year":"1995","journal-title":"Plant J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.plantsci.2004.04.005","article-title":"Co-ordinate expression of \u03b2- and \u03b4-zeins in transgenic tobacco","volume":"167","author":"Randall","year":"2004","journal-title":"Plant Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0168-9452(02)00067-5","article-title":"A linear model for quantitating the accumulation of zeins and their fractions (\u03b1+\u03b4, \u03b2&\u03b3) in developing endosperm of wild-type and mutant maizes","volume":"163","author":"Landry","year":"2002","journal-title":"Plant Sci."}],"container-title":["Agronomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4395\/13\/12\/2912\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:31:25Z","timestamp":1760131885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4395\/13\/12\/2912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,27]]},"references-count":71,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["agronomy13122912"],"URL":"https:\/\/doi.org\/10.3390\/agronomy13122912","relation":{},"ISSN":["2073-4395"],"issn-type":[{"value":"2073-4395","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,27]]}}}