{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:09Z","timestamp":1787027469206,"version":"build-2736575974"},"reference-count":85,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T00:00:00Z","timestamp":1578182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Elective gonadectomy in the dog is a topic of interest for clinicians, pet-owners, and society. Although canine prostatic neoplasia (CPN) has a low incidence (0.35%), reports of an increased risk for castrated dogs attract attention and cause concern in pet-owners. Our aim is to provide professionals and non-professionals with a detailed description of this possible side effect of gonadectomy in the dog. The mean age at diagnosis of CPN ranges from 8.5 to 11.2 years. Medium to large size breeds are more frequently affected. Symptoms and findings of non-invasive examinations are not pathognomonic, therefore, cytological or histological examinations are needed for diagnosis. Overall, the incidence of metastasis reaches up to 80%, yet lung metastasis reportedly has no negative impact on median survival time (MST). It has been reported that castrated males have a significantly higher MST than intact males. Differences in inclusion criteria for studied populations make a comparison of studies difficult. Citation of odds ratios without consideration of the context of the reference may result in premature conclusions. We conclude that elective gonadectomy of adult male dogs under six years of age cannot be excluded from the veterinary practice due to concern of causing CPN until clear and strong evidence is available.<\/jats:p>","DOI":"10.3390\/ani10010085","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T03:48:48Z","timestamp":1578282528000},"page":"85","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Prostatic Neoplasia in the Intact and Castrated Dog: How Dangerous is Castration?"],"prefix":"10.3390","volume":"10","author":[{"given":"Magdalena","family":"Schrank","sequence":"first","affiliation":[{"name":"Department of Animal Medicine, Production and Health (MAPS), University of Padua, 35122 Padova PD, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Romagnoli","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Production and Health (MAPS), University of Padua, 35122 Padova PD, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1136\/vr.109.4.71","article-title":"Fifteen cases of prostatic carcinoma in the dog","volume":"109","author":"Weaver","year":"1981","journal-title":"Vet. Rec."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1038\/sj.pcan.4500825","article-title":"Impact of diet on prostate cancer: A review","volume":"8","author":"Sonn","year":"2005","journal-title":"Prostate Cancer Prostatic Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1090-0233(98)80059-2","article-title":"Host related risk factors for canine osteosarcoma","volume":"156","author":"Ru","year":"1998","journal-title":"Vet. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1939-1676.1999.tb01136.x","article-title":"Cardiac tumors in dogs: 1982\u20131995","volume":"13","author":"Ware","year":"1999","journal-title":"J. Vet. Intern. Med."},{"key":"ref_5","unstructured":"Withrow, S.J., and MacEwen, E.G. (2001). Tumors of the male reproductive system. Small Animal Clinical Oncology, Saunders. [3rd ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0303-7207(02)00261-7","article-title":"Canine prostate carcinoma: Epidemiological evidence of an increased risk in castrated dogs","volume":"197","author":"Teske","year":"2002","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"591753","DOI":"10.1155\/2009\/591753","article-title":"Hormonal and sex impact on the epidemiology of canine lymphoma","volume":"2009","author":"Villamil","year":"2009","journal-title":"J. Cancer Epidemiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"210","DOI":"10.5326\/JAAHA-MS-5621","article-title":"Cutaneous MCTs: Associations with spay\/neuter status, breed, body size, and phylogenetic cluster","volume":"47","author":"White","year":"2011","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.2460\/javma.231.11.1665","article-title":"Determining the optimal age for gonadectomy of dogs and cats","volume":"231","year":"2007","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.cvsm.2014.06.003","article-title":"The role of neutering in cancer development","volume":"44","author":"Smith","year":"2014","journal-title":"Vet. Clin. Small Anim."},{"key":"ref_11","first-page":"171","article-title":"Current perspectives on the optimal age to spay\/castrate dogs and cats","volume":"6","author":"Howe","year":"2015","journal-title":"Vet. Med. Res. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.2460\/javma.250.10.1155","article-title":"A literature review on the welfare implications of gonadectomy of dogs","volume":"250","author":"Houlihan","year":"2017","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.cvsm.2018.02.013","article-title":"Population control in small animals","volume":"48","year":"2018","journal-title":"Vet. Clin. Small Anim."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1439-0531.2012.02078.x","article-title":"Effects of surgical sterilization on canine and feline health and on society","volume":"47","year":"2012","journal-title":"Reprod. Dom. Anim."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1002\/pros.20590","article-title":"A population study of neutering status as a risk factor for canine prostate cancer","volume":"67","author":"Bryan","year":"2007","journal-title":"Prostate"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/(SICI)1097-0045(19980801)36:3<189::AID-PROS7>3.0.CO;2-C","article-title":"High grade prostatic intraepithelial neoplasia in military working dogs with and without prostate cancer","volume":"36","author":"Aquilina","year":"1998","journal-title":"Prostate"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.theriogenology.2015.07.029","article-title":"Contrast-enhanced ultrasonographic characteristics of the diseased canine prostate gland","volume":"84","author":"Troisi","year":"2015","journal-title":"Theriogenology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.rvsc.2017.05.007","article-title":"Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time","volume":"114","author":"Donato","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1699-0463.1954.tb00886.x","article-title":"A statistical investigation of carcinoma in the dog","volume":"35","author":"Krook","year":"1954","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1002\/(SICI)1097-0045(19980615)36:1<64::AID-PROS12>3.0.CO;2-G","article-title":"Workgroup 4: Spontaneous Prostate Carcinoma in Dogs and Nonhuman Primates","volume":"36","author":"Waters","year":"1998","journal-title":"Prostate"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1053\/svms.2000.7304","article-title":"Prostatic Surgery in the Dog","volume":"15","author":"White","year":"2000","journal-title":"Clin. Tech. Small Anim. Pract."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/vco.12400","article-title":"Outcome and prognostic factors in medically treated canine prostatic carcinomas: A multi-institutional study","volume":"16","author":"Ravicini","year":"2018","journal-title":"Vet. Comp. Oncol."},{"key":"ref_23","first-page":"647","article-title":"Prostatic carcinoma in dogs","volume":"5","author":"Hargis","year":"1983","journal-title":"Compend. Contin. Educ. Vet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.2460\/javma.1992.200.08.1119","article-title":"Study of prostatic disease in dogs: 177 cases (1981\u20131986)","volume":"200","author":"Krawiec","year":"1992","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_25","first-page":"239","article-title":"Carcinoma of the prostate gland in dogs: A report of three cases","volume":"99","author":"Schlotthauer","year":"1941","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0368-1742(62)80037-X","article-title":"Studies on the canine prostate gland. Factors influencing its size and weight","volume":"72","year":"1962","journal-title":"J. Comp. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1002\/1097-0142(196811)22:6<1329::AID-CNCR2820220633>3.0.CO;2-X","article-title":"Adenocarcinoma of the canine prostate","volume":"22","author":"Leav","year":"1968","journal-title":"Cancer"},{"key":"ref_28","first-page":"284","article-title":"Prostatic disease in the dog","volume":"68","author":"Hornbuckle","year":"1978","journal-title":"Cornell Vet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1939-1676.1987.tb02013.x","article-title":"The influence of castration on the development of prostatic carcinoma in the dog 43 Cases (1978\u20131985)","volume":"1","author":"Obradovich","year":"1987","journal-title":"J. Vet. Intern. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.2460\/javma.1991.199.11.1623","article-title":"Clinical and pathologic features of prostatic adenocarcinoma in sexually intact and castrated dogs: 31 cases (1970\u20131987)","volume":"199","author":"Bell","year":"1991","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1939-1676.1995.tb03288.x","article-title":"Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders","volume":"9","author":"Bell","year":"1995","journal-title":"J. Vet. Intern. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/1097-0045(20001001)45:2<173::AID-PROS12>3.0.CO;2-R","article-title":"Clinical and pathologic aspects of spontaneous canine prostate carcinoma: A retrospective analysis of 76 Cases","volume":"45","author":"Cornell","year":"2000","journal-title":"Prostate"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/pros.1100","article-title":"Role of canine basal cells in postnatal prostatic development, induction of hyperplasia, and sex hormone-stimulated growth; and the ductal origin of carcinoma","volume":"48","author":"Leav","year":"2001","journal-title":"Prostate"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1046\/j.1476-5829.2003.00007.x","article-title":"Immunohistochemical characterization of canine prostatic carcinoma and correlation with castration status and castration time","volume":"1","author":"Sorenmo","year":"2003","journal-title":"Vet. Comp. Oncol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1354\/vp.41-2-131","article-title":"Canine prostate carcinomas express markers of urothelial and prostatic differentiation","volume":"41","author":"LeRoy","year":"2004","journal-title":"Vet. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1002\/pros.10338","article-title":"Canine prostatic intarepithelial neoplasia: Is the comparative model relevant?","volume":"58","author":"Madewell","year":"2004","journal-title":"Prostate"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1939-165X.2004.tb00365.x","article-title":"Evaluation of the cytologic diagnosis of canine prostatic disorders","volume":"33","author":"Powe","year":"2004","journal-title":"Vet. Clin. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.rvsc.2014.11.006","article-title":"A retrospective analysis of 111 canine prostatic samples: Histopathological findings and classification","volume":"97","author":"Palmieri","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.theriogenology.2015.10.030","article-title":"A retrospective study of canine prostatic diseases from 2002 to 2009 at the Alfort Veterinary College in France","volume":"85","author":"Polisca","year":"2016","journal-title":"Theriogenology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/vsu.12768","article-title":"Total prostatectomy as a treatment for prostatic carcinoma in 25 dogs","volume":"47","author":"Bennett","year":"2018","journal-title":"Vet Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0378-4320(00)00101-9","article-title":"Disorders of the canine prostate","volume":"60\u201361","author":"Johnston","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.tvjl.2008.07.012","article-title":"Prostate cancer in dogs: Comparative and clinical aspects","volume":"180","author":"LeRoy","year":"2009","journal-title":"Vet. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.rvsc.2015.09.004","article-title":"Proposal of Gleason-like grading system of canine prostate carcinoma in veterinary pathology practice","volume":"103","author":"Palmieri","year":"2015","journal-title":"Res. Vet. Sci."},{"key":"ref_44","first-page":"791","article-title":"Diseases of the canine prostate gland","volume":"17","author":"Dorfman","year":"1995","journal-title":"Compend. Contin. Educ. Vet."},{"key":"ref_45","unstructured":"Thrall, D.E. (1986). The prostate. Textbook of Veterinary Diagnostic Radiology, Elsevier Saunders."},{"key":"ref_46","first-page":"1027","article-title":"Canine prostatic disease: Comparison of ultrasonographic appearance with morphologic and microbiologic findings","volume":"190","author":"Feeney","year":"1987","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/rda.12296","article-title":"Diagnosis of common prostatic conditions in dogs: An update","volume":"49","author":"Levy","year":"2014","journal-title":"Reprod. Dom. Anim."},{"key":"ref_48","first-page":"99","article-title":"Noninfectious prostatic diseases in dogs","volume":"24","author":"Gobello","year":"2002","journal-title":"Compend. Contin. Educ. Pract. Vet. N. A. Ed."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.theriogenology.2008.04.039","article-title":"Canine prostatic disease: A review of anatomy, pathology, diagnosis, and treatment","volume":"70","author":"Smith","year":"2008","journal-title":"Theriogenology"},{"key":"ref_50","unstructured":"Ling, G.V. (1995). Diagnostic methods in prostatic disease. Lower Urinary Tract Diseases of Dogs and Cats, Mosby."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.theriogenology.2006.05.003","article-title":"Collection of tissue and culture samples from the canine reproductive tract","volume":"66","year":"2006","journal-title":"Theriogenology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/j.1740-8261.2002.tb00443.x","article-title":"Needle-tract implantation following ultrasound-guided fine-needle aspiration biopsy of transitional cell carcinoma of the bladder, urethra and prostate","volume":"43","author":"Nyland","year":"2002","journal-title":"Vet. Radiol. Ultrasound"},{"key":"ref_53","first-page":"235","article-title":"Zur Aussagekraft der Zytologie bei der Diagnostik des Prostatakarzinomas beim Hund","volume":"41","author":"Teske","year":"1996","journal-title":"Kleintierpraxis"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.17221\/5511-VETMED","article-title":"Diagnostic possibilities in the management of canine prostatic disorders","volume":"51","author":"Paclikova","year":"2006","journal-title":"Vet. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1002\/pros.20720","article-title":"Histopathological and Immunohistochemical Characterization of Canine Prostate Cancer","volume":"68","author":"Lai","year":"2008","journal-title":"Prostate"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Fonseca-Alves, C.E., Kobayashi, P.E., Rivera Calder\u00f3n, L.G., Felisbino, S.L., de Carvalho Rinaldi, J., Drigo, S.A., Rogatto, S.R., and Laufer-Amorim, R. (2017). Immunohistochemical panel to characterize canine prostate carcinomas according to aberrant p63 expression. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0199173"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1053\/j.tcam.2018.09.002","article-title":"Advances in prostatic diagnostics in dogs: The role of canine prostatic specific esterase in the early diagnosis of prostatic disorders","volume":"33","author":"Alonge","year":"2018","journal-title":"Top. Companion Anim. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.theriogenology.2017.01.032","article-title":"The association between the serum concentration of canine prostate specific esterase (CPSE) and the size of the canine prostate","volume":"93","author":"Holst","year":"2017","journal-title":"Theriogenology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1111\/j.1532-950X.2006.00165.x","article-title":"Partial prostatectomy using Nd:YAG laser for management of canine prostate carcinoma","volume":"35","author":"Kirpensteijn","year":"2006","journal-title":"Vet. Surg."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/(SICI)1097-0045(20000101)42:1<73::AID-PROS9>3.0.CO;2-G","article-title":"Overexpression of cyclooxygenase-2 in human prostate adenocarcinoma","volume":"42","author":"Gupta","year":"2000","journal-title":"Prostate"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1093\/jnci\/91.16.1398","article-title":"Induction of prostaglandin G\/H synthase-2 in a canine model of spontaneous prostatic adenocarcinoma","volume":"91","author":"Tremblay","year":"1999","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1354\/vp.42-1-100","article-title":"Estrogen-dependent induction of cyclooxygenase-2 in the canine prostate in vivo","volume":"42","author":"Chevalier","year":"2005","journal-title":"Vet. Pathol."},{"key":"ref_63","first-page":"125","article-title":"Classification of prostatic carcinomas","volume":"50","author":"Gleason","year":"1966","journal-title":"Cancer Chemother. Pharm."},{"key":"ref_64","first-page":"293","article-title":"Studies of prostatic cancer: I. Effect of castration, estrogen and androgen injections on serum phosphatases in metastatic carcinoma of the prostate","volume":"1","author":"Huggins","year":"1941","journal-title":"Cancer Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3322\/canjclin.45.1.8","article-title":"Cancer statistics","volume":"45","author":"Wingo","year":"1995","journal-title":"CA Cancer J. Clin."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3322\/caac.20107","article-title":"Global cancer statitics","volume":"61","author":"Jemal","year":"2011","journal-title":"CA Cancer J. Clin."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1093\/jnci\/88.22.1686-b","article-title":"Comparing the age at prostate cancer diagnosis in humans and dogs","volume":"88","author":"Waters","year":"1996","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/pros.22642","article-title":"A novel canine model for prostate cancer","volume":"73","author":"Keller","year":"2013","journal-title":"Prostate"},{"key":"ref_69","unstructured":"DeVita, V.T., Hellman, S., and Rosenberg, S.A. (1985). Complete androgen blockade for the treatment of prostate cancer. Important Advanced in Oncology, J. B. Lippincott."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1507\/endocrj.K07-009","article-title":"Effect of castration on extracellular matrix remodeling and angiogenesis of the prostate gland","volume":"54","author":"Shidaifat","year":"2007","journal-title":"Endocr. J."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Meuten, D.J. (2002). Tumors of the genital system. Tumors in Domestic Animals, Iowa State University Press. [4th ed.].","DOI":"10.1002\/9780470376928"},{"key":"ref_72","first-page":"948","article-title":"Maximum-distention retrograde urethrocystography in healthy male dogs: Occurrence and radiographic appearance of urethroprostatic reflux","volume":"45","author":"Feeney","year":"1984","journal-title":"Am. J. Vet. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1097\/01.pas.0000173646.99337.b1","article-title":"The 2005 international society of urological pathology (ISUP) consensus conference on gleason grading of prostatic carcinoma","volume":"29","author":"Epstein","year":"2005","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/j.1464-410X.2012.11611.x","article-title":"Prognostic Gleason grade grouping: Data based on the modified Gleason scoring system","volume":"111","author":"Pierorazio","year":"2013","journal-title":"BJU Int."},{"key":"ref_75","unstructured":"Romagnoli, S. Surgical gonadectomy in the bitch and queen: Should it be done and at what age? In Proceedings of the Southern European Veterinary Conference and Congreso Nacional AVEPA, Barcelona, Spain, 17\u201319 October 2008."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1071\/RD07088","article-title":"Pituitary and testicular endocrine responses to exogenous gonadotrophin-releasing hormone (GnRH) and luteinising hormone in male dogs treated with GnRH agonist implants","volume":"19","author":"Junaidi","year":"2007","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_77","unstructured":"Ettinger, S.J., and Feldman, E.C. (2005). Prostatic diseases. Textbook of Veterinary Internal Medicine, Saunders Elsevier. [6th ed.]."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1002\/pros.2990050505","article-title":"Single case report of prostate adenocarcinoma in a dog castrated three months previously. Morphological, biochemical, and endocrine determinations","volume":"5","author":"Frenette","year":"1984","journal-title":"Prostate"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/1097-0045(20000615)44:1<40::AID-PROS6>3.0.CO;2-J","article-title":"Cell kinetics and differentiation after hormonal-induced prostatic hyperplasia in the dog","volume":"44","author":"Mahapokai","year":"2000","journal-title":"Prostate"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Morgan, R.V. (2008). Diseases of the prostate. Handbook of Small Animal Practice, Saunders Elsevier. [5th ed.].","DOI":"10.1016\/B978-1-4160-3949-5.50042-X"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/j.1748-5827.2011.01176.x","article-title":"The effect of neutering on the risk of urinary incontinence in bitches\u2014A systematic review","volume":"53","author":"Beauvais","year":"2012","journal-title":"J. Small Anim. Pract."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"380","DOI":"10.2460\/javma.2004.224.380","article-title":"Long-term risks and benefits of early-age gonadectomy in dogs","volume":"244","author":"Spain","year":"2004","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Torres de la Riva, G., Hart, B.L., Farver, T.B., Oberbauer, A.M., Messam, L.L., Willits, N., and Hart, L.A. (2013). Neutering dogs: Effects on joint disorders and cancers in golden retrievers. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055937"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"309","DOI":"10.2460\/javma.244.3.309","article-title":"Evaluation of the risk and age of onset of cancer and behavioral disorders in gonadectomized Viszlas","volume":"244","author":"Zink","year":"2014","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_85","first-page":"81","article-title":"A retrospective study of 87 cases of canine soft tissue sarcomas, 1986\u20132001","volume":"3","author":"Heller","year":"2005","journal-title":"Intern. J. Appl. Res. Vet. Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/85\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:42:19Z","timestamp":1760362939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/85"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,5]]},"references-count":85,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ani10010085"],"URL":"https:\/\/doi.org\/10.3390\/ani10010085","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,5]]}}}