{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:20:26Z","timestamp":1791663626532,"version":"4.3.4"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003193","name":"Ministerstvo \u0161kolstva, vedy, v\u00fdskumu a \u0161portu Slovenskej republiky","doi-asserted-by":"publisher","award":["VEGA 2\/0006\/17"],"award-info":[{"award-number":["VEGA 2\/0006\/17"]}],"id":[{"id":"10.13039\/501100003193","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The present study investigated the effects of enterocin (Ent) M and sage extract alone and also in combination on the gut microflora, phagocytic activity, blood biochemistry, and morphometry of rabbits. Sixty-four rabbits (aged five weeks, M91 meatline, both sexes) were divided into three experimental groups: E (EntM; 50 \u00b5L\/animal\/day), S (sage; 10 \u00b5L\/animal\/day), and E + S (EntM + sage) groups and control group (C). The additives were administered in drinking water for a period of 21 days. Dietary supplementation of EntM and sage significantly reduced the coliforms (E: p &lt; 0.001; S: p &lt; 0.001; E + S: p &lt; 0.001) in feces, while simultaneous addition of EntM and sage decreased enterococci (E + S: p &lt; 0.0001), lactic acid bacteria (E + S: p &lt; 0.01), and coagulase-positive staphylococci (E + S: p &lt; 0.0001) in the appendix. Sage addition reduced HDL (S: p &lt; 0.001) and LDL cholesterol (S: p &lt; 0.001; E + S: p &lt; 0.001), LDL\/HDL ratio (S: p &lt; 0.001; E + S: p &lt; 0.01), and increased urea (S: p &lt; 0.01; E + S: p &lt; 0.001) and creatinine (S: p &lt; 0.001; E + S: p &lt; 0.001) in serum. EntM and sage application, alone or in combination, improve the jejunal morphometry (p &lt; 0.0001) in rabbits.<\/jats:p>","DOI":"10.3390\/ani10010115","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T10:20:29Z","timestamp":1578651629000},"page":"115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Can Enterocin M in Combination with Sage Extract Have Beneficial Effect on Microbiota, Blood Biochemistry, Phagocytic Activity and Jejunal Morphometry in Broiler Rabbits?"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-4672","authenticated-orcid":false,"given":"Monika","family":"Pog\u00e1ny Simonov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u013dubica","family":"Chrastinov\u00e1","sequence":"additional","affiliation":[{"name":"National Agricultural and Food Centre, Hlohoveck\u00e1 2, 951 41 Nitra-Lu\u017eianky, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kandri\u010d\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So\u0148a","family":"Gancar\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Gnotobiology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Bino","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8724-0159","authenticated-orcid":false,"given":"Iveta","family":"Plach\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"\u0160\u010derbov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viola","family":"Strompfov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"\u017dit\u0148an","sequence":"additional","affiliation":[{"name":"National Agricultural and Food Centre, Hlohoveck\u00e1 2, 951 41 Nitra-Lu\u017eianky, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7413-3816","authenticated-orcid":false,"given":"Andrea","family":"Laukov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, \u0160olt\u00e9sovej 4-6, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.livsci.2016.04.024","article-title":"Herbs and spices inclusion as feedstuff or additive in growing rabbit diet and as additive in rabbit meat: A review","volume":"189","author":"Celia","year":"2016","journal-title":"Livest. Sci."},{"key":"ref_2","first-page":"127","article-title":"Alternatives to antibiotic growth promoters in rabbit feeding: A review","volume":"15","author":"Maertens","year":"2007","journal-title":"World Rabbit Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.5713\/ajas.2008.70186","article-title":"The effect of probiotics on microbiology, health and performance of fattening rabbits","volume":"21","author":"Kritas","year":"2008","journal-title":"Asian Australas. J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"376","DOI":"10.17221\/1659-CJAS","article-title":"Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in thedigestive tract of rabbits","volume":"54","author":"Faix","year":"2009","journal-title":"Czech J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1080\/09712119.2016.1174126","article-title":"Effect of probiotic supplementation on growth performance, nutrientutilization and carcass characteristics of growing Chinchilla rabbits","volume":"45","author":"Bhatt","year":"2017","journal-title":"J. Appl. Anim. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"123","DOI":"10.4995\/wrs.2017.4533","article-title":"Effect of competitive exclusion in rabbits using an autochtonous probiotic","volume":"25","author":"Cunha","year":"2017","journal-title":"World Rabbit Sci."},{"key":"ref_7","first-page":"2811","article-title":"Probiotic supplementation in rabbit: A review","volume":"8","author":"Kalma","year":"2016","journal-title":"Int. J. Agric. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/j.1365-2672.1989.tb05105.x","article-title":"Probiotics in man and animals","volume":"66","author":"Fuller","year":"1989","journal-title":"J. Appl. Bacteriol."},{"key":"ref_9","first-page":"20","article-title":"Production of anti-microbial substances by probiotics","volume":"5","author":"Mishra","year":"1996","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/1097-0282(2000)55:1<50::AID-BIP50>3.0.CO;2-3","article-title":"Class II antimicrobial peptides from lactic acid bacteria","volume":"55","author":"Nes","year":"2000","journal-title":"Biopolymers"},{"key":"ref_11","first-page":"924","article-title":"Applications of bacteriocins in food, livestock health and medicine","volume":"3","author":"Bemena","year":"2014","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.copbio.2017.07.011","article-title":"Functions and emerging applications of bacteriocins","volume":"49","author":"Chikindas","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12602-014-9156-4","article-title":"Beneficial effect of lantibiotic nisin in rabbit husbandry","volume":"6","year":"2014","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/j.1574-6976.2007.00064.x","article-title":"Diversity of enterococcal bacteriocins and their grouping in a new classification scheme","volume":"31","author":"Franz","year":"2007","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_15","first-page":"15","article-title":"Applications of bacteriocins in livestock","volume":"8","year":"2007","journal-title":"Curr. Issues Intest. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12602-012-9118-7","article-title":"Enterococcus faecium AL 41: Its enterocin M and their beneficial use in rabbits husbandry","volume":"4","year":"2012","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_17","first-page":"359","article-title":"Effect of combined administration of enterocin 4231 and sage in rabbits","volume":"14","year":"2011","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s12223-015-0423-x","article-title":"Benefits of combinative application of probiotic, enterocin M producing strain Enterococcus faecium AL41 and Eleutherococcus senticosus in rabbits","volume":"61","year":"2016","journal-title":"Folia Microbiol."},{"key":"ref_19","first-page":"581","article-title":"Experimental application of sage in rabbit husbandry","volume":"7","author":"Faix","year":"2008","journal-title":"Acta Vet. Brno"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0377-8401(92)90029-6","article-title":"Prediction of the digestible energy and digestibility of gross energy of feed for rabbits. 1. Individual classes of feeds","volume":"39","author":"Wiseman","year":"1992","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_21","unstructured":"Cunniff, P.A. (1995). Official Methods of Analysis. Association of Official Analytical Chemists, AOAC. [16th ed.]."},{"key":"ref_22","first-page":"57","article-title":"Technical note: Attempts to harmonize chemical analyses of feeds and faeces for rabbit feed evaluation","volume":"9","author":"Gidenne","year":"2001","journal-title":"World Rabbit Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","article-title":"Methods for dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition","volume":"74","author":"Robertson","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s10295-007-0226-4","article-title":"Isolation and characterization of a new bacteriocins produced by enviromental isolate Enterococcus faecium AL41","volume":"34","author":"Skaugen","year":"2007","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_25","first-page":"9","article-title":"Characterization of the antagonistic activity of Lactobacillus amylovorus DCE471 and large scale isolation of its bacteriocin amylovorin L471","volume":"9","author":"Callewaert","year":"1996","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/S0021-9258(20)78300-6","article-title":"The colorimetric determination of the serum proteins","volume":"82","author":"Greenberg","year":"1929","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0009-8981(73)90466-X","article-title":"Determination of serum creatinine by a direct colorimetric method","volume":"43","year":"1973","journal-title":"Clin. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1136\/jcp.14.4.418","article-title":"A sensitive method for the colorimetric determination of urea","volume":"14","author":"Beale","year":"1961","journal-title":"J. Clin. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/0009-8981(77)90357-6","article-title":"A new colorimetric method for determination of serum glucose","volume":"75","author":"Sugiura","year":"1977","journal-title":"Clin. Chim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0009-8981(77)90003-1","article-title":"A simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase","volume":"81","author":"Sugiura","year":"1977","journal-title":"Clin. Chim. Acta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0021-9258(18)72150-9","article-title":"The colorimetric determination of cholesterol","volume":"150","author":"Sperry","year":"1943","journal-title":"J. Biol. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1093\/ajcp\/28.1.56","article-title":"A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases","volume":"28","author":"Reitman","year":"1957","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0021-9258(18)56039-7","article-title":"The colorimetric determination of phosphatases in human serum","volume":"190","author":"Seligman","year":"1951","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1097\/00000658-194810000-00003","article-title":"Colorimetric determination of amylase","volume":"128","author":"Huggins","year":"1948","journal-title":"Ann. Surg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/0009-8981(57)90063-3","article-title":"The colorimetric estimation of calcium in serum with o-cresolphthalein complexone","volume":"2","author":"Stern","year":"1957","journal-title":"Clin. Chim. Acta"},{"key":"ref_36","first-page":"399","article-title":"Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine","volume":"26","author":"Berti","year":"1988","journal-title":"J. Clin. Chem. Clin. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1042\/bj0600522","article-title":"A colorimetric method for the estimation of serum magnesium","volume":"60","author":"Smith","year":"1955","journal-title":"Biochem. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1093\/clinchem\/10.6.533","article-title":"A colorimetric method for determination of serum chloride","volume":"10","author":"Schoenfeld","year":"1964","journal-title":"Clin. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0165-2478(82)90040-2","article-title":"Phagocytosis of human blood leukocytes, a simple micro-method","volume":"5","author":"Fornousek","year":"1982","journal-title":"Immunol. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"525","DOI":"10.17221\/1968-VETMED","article-title":"Morphology of small intestinal mucosa and intestinal weight change with metabolic type of cattle","volume":"53","author":"Voigt","year":"2008","journal-title":"Vet. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1590\/S0101-20612009000400010","article-title":"Chemical characterization and antimicrobial activity of essential oils of salvia L. species","volume":"29","author":"Pierozan","year":"2009","journal-title":"Ci\u00eanc. Tecnol. Aliment."},{"key":"ref_42","first-page":"1028","article-title":"Assessment of Lantibiotic type bacteriocin\u2014Gallidermin application in model experiment with broiler rabbits","volume":"2","year":"2018","journal-title":"Int. J. Anim. Sci."},{"key":"ref_43","unstructured":"Meredith, A., and Flecknell, P. (2006). Clinical Pathology. Manual of Rabbits Medicine and Surgery, BSAVA. [2nd ed.]."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2754\/avb201281040371","article-title":"Selected haematological and biochemical indicators in different breeds of rabbits","volume":"81","author":"Martinec","year":"2012","journal-title":"Acta Vet. Brno"},{"key":"ref_45","first-page":"253","article-title":"Normal values of haematological and some biochemical parameters in serum and urine of New Zealand rabbits","volume":"20","author":"Kaya","year":"2012","journal-title":"World Rabbit Sci."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Pog\u00e1ny Simonov\u00e1, M., Chrastinov\u00e1, \u013d., Kandri\u010d\u00e1kov\u00e1, A., Kuba\u0161ov\u00e1, I., Formelov\u00e1, Z., Chrenkov\u00e1, M., Miltko, R., Belzecki, G., Strompfov\u00e1, V., and Laukov\u00e1, A. (2019). Enterocin M and sage supplementation in post-weaning rabbits: Effects on growth performance, caecal microbiota, fermentation and enzymatic activity. Probiotics Antimicrob. Proteins.","DOI":"10.1007\/s12602-019-09584-z"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jep.2010.07.054","article-title":"Activation of the nuclear receptor PPAR\u03b3 by metabolites isolated from sage (Salvia officinalis L.)","volume":"132","author":"Christensen","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1042\/bj1640185","article-title":"The mechanism of inhibition by acidosis of gluconeogenesis from lactate in rat liver","volume":"164","author":"Illes","year":"1977","journal-title":"Biochem. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1053\/saep.2002.125100","article-title":"Calcium metabolism in rabbits","volume":"Volume 11","author":"Redrobe","year":"2002","journal-title":"Seminars in Avian and Exotic Pet Medicine"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"145","DOI":"10.4081\/ijas.2012.e27","article-title":"Effect of spray application of Lactobacillus plantarum on in vivo performance, caecal fermentations and haematological traits of suckling rabbits","volume":"11","author":"Bovera","year":"2012","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.jtcme.2016.12.014","article-title":"Pharmacological properties of Salvia officinalis and its components","volume":"7","author":"Ghorbani","year":"2017","journal-title":"J. Tradit. Complement. Med."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Pog\u00e1ny Simonov\u00e1, M., Laukov\u00e1, A., Plach\u00e1, I., \u010cobanov\u00e1, K., Strompfov\u00e1, V., Szab\u00f3ov\u00e1, R., and Chrastinov\u00e1, \u013d. Can enterocins affect phagocytosis and glutathione peroxidase in rabbits?. Cent. Eur. J. Biol., 8, 730\u2013734.","DOI":"10.2478\/s11535-013-0198-x"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1006\/fsim.2002.0425","article-title":"Imunomodulatory effects of nisin in turbot (Scophthalmus maximus L.)","volume":"14","author":"Villamil","year":"2003","journal-title":"Fish Shelfish Immunol."},{"key":"ref_54","unstructured":"Brzozowski, T. (2013). The impact of probiotics on the gastrointestinal physiology. New Advances in the Basic and Clinical Gastroenterology, InTech."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.livsci.2013.06.017","article-title":"Growth performance, apparent nutrient digestibility, caecal fermentation, ileal morphology and caecal microflora of growing rabbits fed diets containing probiotics and prebiotics","volume":"157","author":"Oso","year":"2013","journal-title":"Livest. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"509","DOI":"10.17221\/8559-CJAS","article-title":"Effect of rabbit origin enterocin-producing strain Enterococcus faecium CCM7420 application on growth performance and gut morphometry in rabbits","volume":"60","year":"2015","journal-title":"Czech J. Anim. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/115\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:42:40Z","timestamp":1760362960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,10]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ani10010115"],"URL":"https:\/\/doi.org\/10.3390\/ani10010115","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,10]]}}}