{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T02:12:37Z","timestamp":1793758357252,"version":"4.6.1"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Virbac Pty Ltd, World Horse Welfare, Goldners Horse Transport","award":["na"],"award-info":[{"award-number":["na"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The regulations for minimal space and direction of travel for land transport in horses vary worldwide and there is currently no definitive guidance to promote equine health and welfare. This study evaluated the effects of bay size and direction of travel (forwards\/backwards) in horses by comparing the behavioural, physiological, laboratory and gastroscopy parameters between transported and confined horses. A total of twenty-six mares took part in the study; 12 horses were confined for 12 h, and all mares underwent 12 hours\u2019 transportation, travelling in single (n = 18) or wide bays (n = 8), and forward (n = 10) or rear (n = 16) facing. Behaviour was recorded during confinement\/transportation and analysed using a behaviour sampling ethogram. Clinical examination, blood samples and gastroscopy were conducted before and after confinement\/transportation. The frequency of behaviours relating to stress and balance increased during transport, and horses transported in a rear-facing position and in a wider bay size showed fewer balance-related behaviours. Balance behaviours, particularly loss of balance, were positively associated with the severity of gastric ulceration after transportation and elevated muscle enzymes, while increased stress behaviours correlated with decreased gastrointestinal sounds. Heart rate and rectal temperature after transportation were positively associated with balance and stress behaviours, and with squamous gastric ulcer scores. Transportation was associated with expected increases in cortisol and muscle enzymes, but positioning and space allowance had minimal effects on these analytes. Findings suggest that transportation in a rear-facing position and in wider bays might reduce the impact of transport on horse health and welfare, and monitoring behaviour in transit and physiological measurements after transportation should be recommended. Behavioural and physiological parameters were more sensitive than haematological, biochemical or endocrine analytes to identify horses suffering from transport stress.<\/jats:p>","DOI":"10.3390\/ani10010160","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T07:39:02Z","timestamp":1579246742000},"page":"160","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Effects of Transport Conditions on Behavioural and Physiological Responses of Horses"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7630-8285","authenticated-orcid":false,"given":"Barbara","family":"Padalino","sequence":"first","affiliation":[{"name":"Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharanne L","family":"Raidal","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences, Charles Stuart University, Wagga, NSW 2650, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Padalino, B., Raidal, S.L., Hall, E., Knight, P., Celi, P., Jeffcott, L., and Muscatello, G. (2016). A survey on transport management practices associated with injuries and health problems in horses. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0162371"},{"key":"ref_2","first-page":"92","article-title":"Effects of vehicle type, driver experience and transport management during loading and in-transit on the welfare of road transported horses in New Zealand","volume":"78","author":"Riley","year":"2018","journal-title":"N. Z. J. Anim. Sci. Prod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3389\/fvets.2018.00294","article-title":"A Survey-based investigation of human factors associated with transport related injuries in horses","volume":"5","author":"Padalino","year":"2018","journal-title":"Front. Vet. Sci."},{"key":"ref_4","first-page":"523","article-title":"Injuries in horses transported to slaughter in Canada","volume":"95","author":"Roy","year":"2015","journal-title":"Can. J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Padalino, B., Raidal, S.L., Knight, P., Celi, P., Jeffcott, L., and Muscatello, G. (2018). Behaviour during transportation predicts stress response and lower airway contamination in horses. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194272"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"697","DOI":"10.2460\/javma.1996.208.05.697","article-title":"Risk factors for history of previous colic and for chronic, intermittent colic in a population of horses","volume":"208","author":"Cohen","year":"1996","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_7","unstructured":"Padalino, B., Davis, G., and Raidal, S. Effects of transportation on gastric pH and gastric ulceration in mares. J. Vet. Intern. Med., Accepted."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.prevetmed.2011.04.010","article-title":"Risk assessment in animal welfare\u2014Especially referring to animal transport","volume":"102","author":"Marahrens","year":"2011","journal-title":"Prev. Vet. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2754\/avb201685030303","article-title":"Pre-transport factors affecting the welfare of cattle during road transport for slaughter\u2014A review","volume":"85","author":"Passantino","year":"2016","journal-title":"Acta Vet. Brno"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.meatsci.2012.04.010","article-title":"Road transport of cattle, swine and poultry in North America and its impact on animal welfare, carcass and meat quality: A review","volume":"92","author":"Faucitano","year":"2012","journal-title":"Meat Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.jveb.2015.02.002","article-title":"Effects of the different transport phases on equine health status, behavior, and welfare: A review","volume":"10","author":"Padalino","year":"2015","journal-title":"J. Vet. Behav. Clin. Appl. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0168-1591(93)90018-K","article-title":"The effect of orientation during trailer transport on heart rate, cortisol and balance in horses","volume":"38","author":"Clark","year":"1993","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1136\/vr.139.1.7","article-title":"Effects of transporting horses facing either forwards or backwards on their behaviour and heart rate","volume":"139","author":"Waran","year":"1996","journal-title":"Vet. Rec."},{"key":"ref_14","unstructured":"Cregier, S.E. (1984, January 1). Reducing equine hauling stress. Proceedings of the International Congress on Applied Ethology in Farm Animals, Kiel, Gwermany."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.jveb.2011.09.003","article-title":"Effects of different positions during transport on physiological and behavioral changes of horses","volume":"7","author":"Padalino","year":"2012","journal-title":"J. Vet. Behav. Clin. Appl. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.4141\/A98-078","article-title":"Maximum loading density of loose horses","volume":"79","author":"Whiting","year":"1999","journal-title":"Can. J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.jevs.2009.10.005","article-title":"Effects of density and rest stops on movement rates of unrestrained horses during transport","volume":"29","author":"Calabrese","year":"2009","journal-title":"J. Equine Vet. Sci."},{"key":"ref_18","unstructured":"National Avian Welfare Alliance (NAWA) (2011). Animal Welfare (Transport within New Zealand) Code of Welfare 2011."},{"key":"ref_19","unstructured":"(2015, October 01). Eur-Lex. Available online: http:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/?uri=uriserv:f83007."},{"key":"ref_20","unstructured":"(2015, October 01). Land Transport. Available online: http:\/\/www.animalwelfarestandards.net.au\/land-transport\/."},{"key":"ref_21","first-page":"1","article-title":"Staying upright in a moving trailer","volume":"3","author":"Roberts","year":"1990","journal-title":"Equine Athl."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1751-0813.1997.tb14349.x","article-title":"Effect of transportation on lower respiratory tract contamination and peripheral blood neutrophil function","volume":"75","author":"Raidal","year":"1997","journal-title":"Aust. Vet. J."},{"key":"ref_23","unstructured":"(2016, February 14). Practical Guidelines to Assess Fitness for Transport of Equidae. Available online: http:\/\/www.fve.org\/news\/index.php?id=208#208."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.rvsc.2017.01.024","article-title":"Immunological, clinical, haematological and oxidative responses to long distance transportation in horses","volume":"115","author":"Padalino","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jveb.2011.04.007","article-title":"Transport stress in horses: Effects of two different distances","volume":"7","author":"Tateo","year":"2012","journal-title":"J. Vet. Behav. Clin. Appl. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.applanim.2012.05.008","article-title":"A novel scale of behavioural indicators of stress for use with domestic horses","volume":"140","author":"Young","year":"2012","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_27","unstructured":"McGreevy, P. (2012). Equine Behavior: A Guide for Veterinarians and Equine Scientists, Elsevier."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0168-1591(94)00550-X","article-title":"Agonistic ethogram of the equid bachelor band","volume":"43","author":"McDonnell","year":"1995","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jveb.2018.10.001","article-title":"Reliability of a descriptive reference ethogram for equitation science","volume":"29","author":"Pierard","year":"2019","journal-title":"J. Vet. Behav."},{"key":"ref_30","unstructured":"Waring, G.H. (2003). Horse Behavior, William Andrew Publish."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1111\/evj.12691","article-title":"Pharmacokinetic and pharmacodynamic effects of two omeprazole formulations on stomach pH and gastric ulcer scores","volume":"49","author":"Raidal","year":"2017","journal-title":"Equine Vet. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.2042-3306.1999.tb05176.x","article-title":"Efficacy of omeprazole paste in the treatment and prevention of gastric ulcers in horses","volume":"31","author":"Andrews","year":"1999","journal-title":"Equine Vet. J."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Leadon, D.P., and Hodgson, D.R. (2014). Transport of horses. The Athletic Horse: Principles and Practice of Equine Sports Medicine, Elsevier-Saunders. [2nd ed.].","DOI":"10.1016\/B978-0-7216-0075-8.00019-8"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/j.2042-3292.2011.00293.x","article-title":"Welfare issues related to transport and handling of both trained and unhandled horses and ponies","volume":"24","author":"Weeks","year":"2012","journal-title":"Equine Vet. Educ."},{"key":"ref_35","unstructured":"McDonnell, S. (2003). Practical Field Guide to Horse Behavior: The Equid Ethogram, The Blood-Horse Inc."},{"key":"ref_36","unstructured":"Cregier, S.E. (2010). Best practices: Surface transport of the horse. Proceedings of Animal Transportation Association, AATA Education Committee."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1152\/physrev.00010.2018","article-title":"The sleep-immune crosstalk in health and disease","volume":"99","author":"Besedovsky","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e106","DOI":"10.3928\/19382359-20170221-01","article-title":"The value of sleep on athletic performance, injury, and recovery in the young athlete","volume":"46","author":"Copenhaver","year":"2017","journal-title":"Pediatr. Ann."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s40279-014-0260-0","article-title":"Sleep and athletic performance: The effects of sleep loss on exercise performance, and physiological and cognitive responses to exercise","volume":"45","author":"Fullagar","year":"2015","journal-title":"Sports Med."},{"key":"ref_40","first-page":"331","article-title":"Serum enzyme changes and hemato-chemical levels in Thoroughbreds after transport and exercise","volume":"45","author":"Codazza","year":"1974","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Houpt, K.A., and Wickens, C.L. (2014). Handling and transport of horses. Livestock Handling and Transport, CABI. [4th ed.].","DOI":"10.1079\/9781780643212.0315"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1017\/S1357729800054631","article-title":"Effect of driver behaviour, driving events and road type on the stability and resting behaviour of sheep in transit","volume":"79","author":"Cockram","year":"2004","journal-title":"Anim. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1111\/j.1740-0929.2012.01065.x","article-title":"Comparative endocrinological responses to short transportation of Equidae (Equus asinus and Equus caballus)","volume":"84","author":"Fazio","year":"2013","journal-title":"Anim. Sci. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.yhbeh.2009.11.003","article-title":"Cortisol release and heart rate variability in horses during road transport","volume":"57","author":"Schmidt","year":"2010","journal-title":"Horm. Behav."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.jevs.2013.12.008","article-title":"Influence of transportation on the serum concentrations of the cardiac biomarkers troponin I and Creatine Kinase-Myocardial Band (CK-MB) and on cortisol and lactate in horses","volume":"34","author":"Tharwat","year":"2014","journal-title":"J. Equine Vet. Sci."},{"key":"ref_46","unstructured":"Riley, B.C. (2016, January 4\u20136). Mechanical & behavioral responses of horses during non-commercial trailer transport\u2014A pilot study. Proceedings of the 42nd Animal Transport Assciation Conference, Lisbon, Portugal."},{"key":"ref_47","unstructured":"Woods, J., and Messori, S. (2014, January 7). Animal transport legislation and conditions of long distance transport. Proceedings of the CP2 Final Conference, Kurhaus, Merano, Italy."},{"key":"ref_48","unstructured":"Messori, S., Ouweltjes, W., Visser, K., Dalla Villa, P., Spoolder, H., and Baltussen, W. (September, January 29). Improving horse welfare at transport: Definition of good practices through a Delphi procedure. Proceedings of the Book of abstracts of the 67th Annual Meeting of the European Federation of Animal Science, Belfast, UK."},{"key":"ref_49","unstructured":"Consortium of the Animal Transport Guides Project (2019, May 03). Guide to Good Practices for the Transport of Horses Destinated for Slaughter. Available online: http:\/\/animaltransportguides.eu\/wp-content\/uploads\/2016\/05\/Animal-Transport-Guides-Horses-2017.pdf."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0737-0806(82)80025-7","article-title":"Reducing equine hauling stress: A review","volume":"2","author":"Cregier","year":"1982","journal-title":"J. Equine Vet. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.2042-3306.1994.tb04406.x","article-title":"Body position and direction preferences in horses during road transport","volume":"26","author":"Smith","year":"1994","journal-title":"Equine Vet. J."},{"key":"ref_52","first-page":"223","article-title":"Alleviating road transit stress on horses","volume":"3","author":"Cregier","year":"1981","journal-title":"Anim. Regul. Stud."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1294\/jes.7.21","article-title":"Behavior of untethered horses during vehicle transport","volume":"7","author":"Kusunose","year":"1996","journal-title":"J. Equine Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-1591(98)00240-8","article-title":"Horse preference for orientation during transport and the effect of orientation on balancing ability","volume":"63","author":"Gibbs","year":"1999","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.2042-3306.1998.tb05140.x","article-title":"Physiological correlates associated with cribbing behaviour in horses: Changes in thermal threshold, heart rate, plasma \u03b2-endorphin and serotonin","volume":"30","author":"Lebelt","year":"1998","journal-title":"Equine Vet. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0737-0806(06)81843-5","article-title":"Equine transportation problems and some preventives: A review","volume":"15","author":"Mansmann","year":"1995","journal-title":"J. Equine Vet. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.applanim.2012.10.002","article-title":"Behaviour and stress responses in horses with gastric ulceration","volume":"142","author":"Malmkvist","year":"2012","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1111\/jvim.13578","article-title":"European college of equine internal medicine consensus statement\u2014Equine gastric ulcer syndrome in adult horses","volume":"29","author":"Sykes","year":"2015","journal-title":"J. Vet. Intern. Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/160\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:43:51Z","timestamp":1760363031000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/1\/160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,17]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ani10010160"],"URL":"https:\/\/doi.org\/10.3390\/ani10010160","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,17]]}}}